Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,156,615	T→C	intergenic (‑5/+299)	SAUSA300_RS10985 ← / ← SAUSA300_RS10990	membrane protein/transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,156,615	0	T	C	100.0%	12.4 / NA	7	intergenic (‑5/+299)	SAUSA300_RS10985/SAUSA300_RS10990	membrane protein/transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (2/5);  total (2/5)

GAAAGCCACCTGTTAAACAAAATCTTGTTCTTTGCATGACAAAACCTAAGAGTCCCCCGACAATTAAGCCACTAAAAATCATCCACATAATCTTAACCTCTCATTTCCGATAAGTTTAGTATGTATTATAGGTGATGATTTTCTTGTTTTCAACCTTATACACAATATT  >  NC_007793/2156523‑2156691                                                                                             |                                                                             atctgCCACCTGTTAAACAAAATCTTGTTCTTTGCATGACAAAACCTAAGAGTCCCCCGACAATTAAGCCCCTAAAAATCATCCACATAATCCTAACctg                                                                       <  1:4997710/96‑2 (MQ=255)      cgatcTGGTAAACAAAATCTTGTTCTTTGCATGACAAAACCTAAGAGTCCCCCGACAATTAAGCCACTAAAAATCATCCACATAATCCTAACCTCTCaat                                                                  <  1:1175516/96‑3 (MQ=255)                  cAAAATCTTGTTCTTTGCATGACAAAACCTAAGAGTCCCCCGACAATTAAGCCACTAAAAATCATCCACATAATCCTAACCTCTCATTTCCGATAAGttt                                                      <  1:2864577/100‑1 (MQ=255)                   aaaaTCTTGTTCTTTGCATGACAAAACCTAAGAGTCCCCCGACAATTAAGCCACTAAAAATCATCCACATAATCCTAACCTCTCATTTCCGATAAGtttg                                                     <  1:6141017/100‑2 (MQ=255)                                ttGCATGACAAAACCTAAGAGTCCCCCGACAATTAAGCCACTAAAAATCATCCACATAATCCTAACCTCTCATTTCCGATAAGTTTAGTATGTATTATAg                                        >  1:3450769/1‑100 (MQ=255)                                   cATGACAAAACCTAAGAGTCCCCCGACAATTAAGCCACTAAAAATCATCCACATAATCCTAACCTCTCATTTCCGATAAGTTTAGTATGTATTATAGGtg                                     >  1:3769754/1‑100 (MQ=255)                                                                      cACTAAAAATCATCCACATAATCCTAACCTCTCATTTCCGATAAGTTTAGTATGTATTATAGGTGATGATTTTCTTGTTTTCAACCTTATAcacatacta  <  1:6576311/100‑6 (MQ=255)                                                                                             |                                                                             GAAAGCCACCTGTTAAACAAAATCTTGTTCTTTGCATGACAAAACCTAAGAGTCCCCCGACAATTAAGCCACTAAAAATCATCCACATAATCTTAACCTCTCATTTCCGATAAGTTTAGTATGTATTATAGGTGATGATTTTCTTGTTTTCAACCTTATACACAATATT  >  NC_007793/2156523‑2156691

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑