Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,143,497	A→G	D170D (GAT→GAC)	SAUSA300_RS10915 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,143,497	0	A	G	100.0%	20.4 / NA	11	D170D (GAT→GAC)	SAUSA300_RS10915	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (2/9);  total (2/9)

TTGGAATCCCCAGGTTGATCGGGATCTGGTGACGGGTTAGGATTTGGCTTATTAGGGTCTGGTTTTGGATTCGGATTTGGCTTTGGTTTATCTGGATCTGGTTTTGGATCCGGATTTGGCTTATTAGGGTCT  >  NC_007793/2143402‑2143533                                                                                                |                                     ttGGAATCCCCAGGTTGATCGGGATCTGGTGACGGGTTAGGATTTGGCTTATTAGGGTCTGGTTTTGGATTCGGATTTGGCTTTGGTTTatctgggtctg                                  <  1:503376/100‑1 (MQ=255)      aTCCCCAGGTTGATCGGGATCTGGTGACGGGTTAGGATTTGGCTTATTAGGGTCTGGTTTTGTATTCGGATTTGGCTTTGGTTTATCTGGGTCTGGtttt                             <  1:3757524/100‑1 (MQ=255)      aTCCCCAGGTTGATCGGGATCTGGTGACGGGTTAGGATTTGGCTTATTAGGGTCTGGTTTTGGATTCGGATTTGGCTTTGGTTTATCTGGGTCTGGtttt                             <  1:1260045/100‑1 (MQ=255)      aTCCCCAGGTTGATCGGGATCTGGTGACGGGTTAGGATTTGGCTTATTAGGGTCTGGTTTTGGATTCGGATTTGGCTTTGGTTTATCTGGGTCTGGtttt                             <  1:3029102/100‑1 (MQ=255)      aTCCCCAGGTTGATCGGGATCTGGTGACGGGTTAGGATTTGGCTTATTAGGGTCTGGTTTTGGATTCGGATTTGGCTTTGGTTTATCTGGGTCTGGtttt                             <  1:5749827/100‑1 (MQ=255)        ccccAGGTTGATCGGGATCTGGTGACGGGTTAGGATTTGGCTTATTAGGGTCTGGTTTTGGATTCGGATTTGGCTTTGGTTTATCTGGGTCTGGTTTTgg                           <  1:1165658/100‑1 (MQ=255)        ccccAGGTTGATCGGGATCTGGTGACGGGTTAGGATTTGGCTTATTAGGGTCTGGTTTTGGATTCGGATTTGGCTTTGGTTTATCTGGGTCTGGTTTTgg                           <  1:5021693/100‑1 (MQ=255)          ccAGGTTGATCGGGATCTGGTGACGGGTTAGGATTTGGCTTATTAGGGTCTGGTTTTGGATTCGGATTTGGCTTTGGTTTATCTGGGTCTGGTTTTGGAt                         >  1:2796852/1‑100 (MQ=255)                          gatctGACGGGTTAGGATTTGGCTTATTAGGGTCTGGTTTTGGATTCGGATTTGGCTTTGGTTTATCTGGGTCTGGTTTTGGATCCGGTTTTGGtttcag         <  1:1110582/96‑4 (MQ=17)                            ggTGACGGGTTAGGATTTGGCTTATTAGGGTCTGGTTTTGGATTCGGATTTGGCTTTGGTTTATCTGGGTCTGGTTTTGGATCCGGTTTTGGtttcccag       >  1:725212/1‑95 (MQ=18)                              tGACGGGTTAGGATTTGGCTTATTAGGGTCTGGTTTTGGATTCGGATTTGGCTTTGGTTTATCTGGGTCTGGTTTTGGATCCGGTTTTGGtttcgggtta     <  1:1827249/100‑8 (MQ=17)                                 cGGGTTAGGATTTGGCTTATTAGGGTCTGGTTTTGGATTCGGATTTGGCTTTGGTTTATCTGGGTCTGGTTTTGGATCCGGTTTTGGtttcgggttatct  <  1:3186868/100‑11 (MQ=14)                                                                                                |                                     TTGGAATCCCCAGGTTGATCGGGATCTGGTGACGGGTTAGGATTTGGCTTATTAGGGTCTGGTTTTGGATTCGGATTTGGCTTTGGTTTATCTGGATCTGGTTTTGGATCCGGATTTGGCTTATTAGGGTCT  >  NC_007793/2143402‑2143533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑