Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,127,837	G→A	V44V (GTG→GTA)	SAUSA300_RS10840 →	phosphodiesterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,127,837	0	G	A	100.0%	36.3 / NA	15	V44V (GTG→GTA)	SAUSA300_RS10840	phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/4);  total (11/4)

CAATCTTCTTATATTAAAAATAATGATGTCGTAATATTCAATGAAGCATTTGATAATGGTGCATCAGACAAATTATTAAGTAATGTGAAAAAAGAATATCCTTATCAAACACCTGTACTCGGCCGTTCTCAATCAGGGTGGGACAAAACTGAAGGTAGCTACTCATCAACTGTTGC  >  NC_007793/2127751‑2127926                                                                                       |                                                                                          cAATCTTCTTATATTAAAAATAATGATGTCGTAATATTCAATGAAGCATTTGATAATGGTGCATCAGACAAATTATTAAGTAATGTAAAAAAAGAATATc                                                                              >  1:5830908/1‑100 (MQ=255)       tcttATATTAAAAATAATGATGTCGTAATATTCAATGAAGCATTTGATAATGGTGCATCAGACAAATTATTAAGTAATGTAAAAAGAGAATATCCTTATc                                                                        >  1:4823532/1‑100 (MQ=255)       tcttATATTAAAAATAATGATGTCGTAATATTCAATGAAGCATTTGATAATGGTGCATCAGACAAATTATTAAGTAATGTAAAAAAAGAATATCCTTaag                                                                        >  1:975302/1‑98 (MQ=255)       tcttATATTAAAAATAATGATGTCGTAATATTCAATGAAGCATTTGATAATGGTGCATCAGACAAATTATTAAGTAATGTAAAAAAAGAATATCCTTATc                                                                        >  1:1083263/1‑100 (MQ=255)       tcttATATTAAAAATAATGATGTCGTAATATTCAATGAAGCATTTGATAATGGTGCATCAGACAAATTATTAAGTAATGTAAAAAAAGAATATCCTTATc                                                                        >  1:1600191/1‑100 (MQ=255)       tcttATATTAAAAATAATGATGTCGTAATATTCAATGAAGCATTTGATAATGGTGCATCAGACAAATTATTAAGTAATGTAAAAAAAGAATATCCTTATc                                                                        >  1:1856715/1‑100 (MQ=255)       tcttATATTAAAAATAATGATGTCGTAATATTCAATGAAGCATTTGATAATGGTGCATCAGACAAATTATTAAGTAATGTAAAAAAAGAATATCCTTATc                                                                        >  1:53314/1‑100 (MQ=255)       tcttATATTAAAAATAATGATGTCGTAATATTCAATGAAGCATTTGATAATGGTGCATCAGACAAATTATTAAGTAATGTAAAAAAAGAATATCCTTATc                                                                        >  1:6045011/1‑100 (MQ=255)                  aaaTAATGATGTCGTAATATTCAATGAAGCATTTGATAATGGTGCATCAGACAAATTATTAAGTAATGTAAAAAAAGAATATCCTTATCAAACGCCTgaa                                                             <  1:5311052/100‑3 (MQ=255)                  aaaTAATGATGTCGTAATATTCAATGAAGCATTTGATAATGGTGCAACAGACAAATTATTAAGTAATTTAAAAAAAGAATATCCTTATCAAACACCTggt                                                             <  1:3560724/100‑3 (MQ=255)                              cGTAATATTCAATGAAGCATTTGATAATGGTGCATCAGACAAATTATTAAGTAATGTAAAAAAAGAATATCCTTATCAAACACCTGTACTCGGCCGTtct                                                 >  1:2575243/1‑100 (MQ=255)                                           gAAGCATTTGATAATGGTGCATCAGACAAATTATTAAGTAATGTAAAAAAAGAATATCCTTATCAAACGCCTGTACTCGGCCGTTCTCAATCAGGGTggg                                    <  1:4130239/100‑1 (MQ=255)                                                            agCATCAGACAAATTATTAAGTAATGTAAAAAAAGAATATCCTTATCAAACACCTGTACTCGGCCGTTCTCAATCAGGGGAGGACAGATCGGAAgagcac                   >  1:6366187/2‑95 (MQ=255)                                                             gCATCAGACAAATTATTAAGTAATGTAAAAAAAGAATATCCTTATCAAACACCTGTACTCGGCCGTTCTCAATCAGGGTGGGACAAAACTGAAGGTAGcc                  <  1:4409818/100‑2 (MQ=255)                                                                             taAGTAATGTAAAAAAAGAATATCCTTATCAAACACCTGTACTCGGCCGTTCTCAATCAGGGTGGGACAAAACTGAAGGTAGCTACTCATCAACTGTTGc  >  1:2782257/1‑100 (MQ=255)                                                                                       |                                                                                          CAATCTTCTTATATTAAAAATAATGATGTCGTAATATTCAATGAAGCATTTGATAATGGTGCATCAGACAAATTATTAAGTAATGTGAAAAAAGAATATCCTTATCAAACACCTGTACTCGGCCGTTCTCAATCAGGGTGGGACAAAACTGAAGGTAGCTACTCATCAACTGTTGC  >  NC_007793/2127751‑2127926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑