Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,127,455	T→C	S282P (TCA→CCA)	SAUSA300_RS10835 →	integrase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,127,455	0	T	C	100.0%	29.2 / NA	13	S282P (TCA→CCA)	SAUSA300_RS10835	integrase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/2);  total (11/2)

ATGATGGTTACATTTTCAAAGAAGCCGGATCTATAATTAACCTTCATGCTATCAATAATGCTTTGAAATCAGCCTGTAGAGTCAATAATATATCAATTATTACATCGCACGCATTAAGACACACTCATTGTTCTTATTTACTAGCAAAAGGTGTATCTATACATTACATTTCTAAAAGATTAGGTCATA  >  NC_007793/2127363‑2127551                                                                                             |                                                                                                 atgatgGTTACATTTTCAAAGAAGCCGGATCTATAATTAACCTTCAGGCTATCAATAATGCTTTGAAATCAGCCTGTAGAGTCAATAATATACCAattat                                                                                           >  1:4040699/1‑100 (MQ=255)         tACATTTTCAAAGAAGCCGGATCTATAATTAACCTTCAGGCTATCAATAATGCTTTGAAATCAGCCTGTAGAGTCAATAATATACCAATTATTACATcgc                                                                                   <  1:167374/100‑1 (MQ=255)                 cAAAGAAGCCGGATCTATAATTAACCTTCAGGCTATCAATAATGCTTTGAAATCAGCCTGTAGAGTCAATAATATACCAATTATTACATcacagatcgga                                                                           >  1:362592/1‑93 (MQ=255)                 cAAAGAAGCCGGATCTATAATTAACCTTCAGGCTATCAATAATGCTTTGAAATCAGCCTGTAGAGTCAATAATATACCAATTATTACATCGCACGCATTa                                                                           <  1:1103902/100‑1 (MQ=255)                 cAAAGAAGCCGGATCTATAATTAACCTTCAGGCTATCAATAATGCTTTGAAATCAGCCTGTAGAGTCAATAATATACCAATTATTACATCGCACGCATTa                                                                           >  1:6079364/1‑100 (MQ=255)                 aaaaGAAGCCGGATCTATAATTAACCTTCAGGCTATCAATAATGCTTTGAAATCAGCCTGTAGAGTCAATAATATACCAATTATTACATcgcacgagatc                                                                           >  1:4657276/2‑95 (MQ=255)                   aagaagCCGGATCTATAATTAACCTTCAGGCTATCAATAATGCTTTGAAATCAGCCTGTAGAGTCAATAATATACCAATTATTACGTcgcacacagatcg                                                                         >  1:4933369/1‑95 (MQ=255)                    cgaagCCGGATCTATAATTAACCTTCAGGCTATCAATAATGCTTTGAAATCAGCCTGTAGAGTCAATAATATACCAATTATTACATCGCACGCATTAAGa                                                                        >  1:2461043/2‑100 (MQ=255)                    agaagCCGGATCTATAATTAACCTTCAGGCTATCAATAATGCTTTGAAATCAGCCTGTAGAGTCAATAATATACCAATTATTACATcgcatgagatcgga                                                                        >  1:3663415/1‑90 (MQ=255)                                                                     tCAGCCTGTAGAGTCAATAATATACCAATTATTACATCGCACGCATTAAGACACACTCATTGTTCTTATTTACTAGCAAAAGGTGTATCTAtacattaca                       >  1:2713789/1‑100 (MQ=255)                                                                      cAGCCTGTAGAGTCAATAATATACCAATTATTACATCGCACGCATTAAGACACACTCATTGTTCTTATTTACTAGCAAAAGGTGTATCTAtacattacat                      >  1:1859283/1‑100 (MQ=255)                                                                      cAGCCTGTAGAGTCAATAATATACCAATTATTACATCGCACGCATTAAGACACACTCATTGTTCTTATTTACTAGCAAAAGGTGTATCTAtacattacat                      >  1:404458/1‑100 (MQ=255)                                                                       aGCCTGTAGAGTCAATAATCTACCAATTATTACATCGCACGCATTAAGACACACTCATTGTTCTTATTTACTAGCAAAAGGTGTATCTATacattacatt                     >  1:310641/1‑100 (MQ=255)                                                                                          atacCAATTATTACATCGCACGCATTAAGACACACTCATGGTTCTTATTTACTAGCAAAAGGTGTATCTATACATTACATTTCTAAAAGATTAGGTCATa  >  1:2077051/1‑100 (MQ=255)                                                                                             |                                                                                                 ATGATGGTTACATTTTCAAAGAAGCCGGATCTATAATTAACCTTCATGCTATCAATAATGCTTTGAAATCAGCCTGTAGAGTCAATAATATATCAATTATTACATCGCACGCATTAAGACACACTCATTGTTCTTATTTACTAGCAAAAGGTGTATCTATACATTACATTTCTAAAAGATTAGGTCATA  >  NC_007793/2127363‑2127551

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑