Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,127,228	T→C	I206T (ATT→ACT)	SAUSA300_RS10835 →	integrase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,127,228	0	T	C	100.0%	31.5 / NA	14	I206T (ATT→ACT)	SAUSA300_RS10835	integrase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/3);  total (11/3)

GATCACGTCAGAAAGAAGAGAACTCGTTCATCATTAGCGCTATTCATAATGATTTGTACGGGTTGTCGTGTCAGTGGTGCAAGAAATATAAAGATTGAGCATATCAACCAAGTGAAAAACACTATATTTATTGACGAGCGAAAAACCGATACTTCCCCTAGAT  >  NC_007793/2127134‑2127296                                                                                               |                                                                     cctCACGTCAGAAAGAAGAGAACTCGTTCATCATTAGCGTTATTCATAATGATTTGTACGGGTTGTCGTGTCAGTGGTGCAAGAAATATAAAGACTGAGc                                                                 >  1:1391665/3‑100 (MQ=255)  ctCACGTCAGAAAGAAGAGAACTCGTTCATCATTAGCGTTATTCATAATGATTTGTACGGGTTGTCGTGTCAGTGGTGCAAGAAATATAAAGACTGAGCa                                                                >  1:6632157/2‑100 (MQ=255)  aTCACGTCAGAAAGAAGAGAACTCGTTCATCATTAGCGTTATTCATAATGATTTGTACGGGTTGTCGTGTCAGTGGTGCAAGAAATATAAAGACTGAGCa                                                                >  1:4360769/1‑100 (MQ=255)    cACGTCAGAAAGAAGAGAACTCGTTCATCCTTAGCGTTATTCATAATGATTTGTACGGGTTGTCGTGTCAGTGGTGCAAGAAACATAAAGAtcggaagag                                                              >  1:3446662/1‑92 (MQ=255)    cACGTCAGAAAGAAGAGAACTCGTTCATCATTAGCGTTATTCATAATGATTTGTACGGGTTGTCGTGTCAGTGGTGCAAGAAATATAAAGAtcggaagag                                                              >  1:4535392/1‑92 (MQ=255)    cACGTCAGAAAGAAGAGAACTCGTTCATCATTAGCGTTATTCATAATGATTTGTACGGGTTGTCGTGTCAGTGGTGCAAGAAATATAAAGACTGAGCata                                                              >  1:2818988/1‑100 (MQ=255)    cACGTCAGAAAGAAGAGAACTCGTTCATCATTAGCGTTATTCATAATGATTTGTACGGGTTGTCGCGTCAGTGGTGCAAGAAATATAAAGACTGAGCata                                                              >  1:2602620/1‑100 (MQ=255)         cAGAAAGAAGAGAACTCGTTCATCATTAGCGTTATTCATAATGATTTGTACGGGTTGTCGTGTCAGTGGTGCAAGAAATATAAAGACTGAGCATATCAAc                                                         >  1:6505154/1‑100 (MQ=255)           gaaagaaGAGAACTCGTTCATCATTAGCGTTATTCATAATGATTTGTACGGGTTGTCGTGTCAGTGGTGCAAGAAATATAAAGACTGAGCATATCAACCa                                                       <  1:699753/100‑1 (MQ=255)            ctagaagaGAACTCGTTCATCATTAGCGTTATTCATAATGATTTGTACGGGTTGTCGTGTCAGTGGTGCAAGAAATATAAAGACTGAGCATATCAACCaa                                                      >  1:2805157/3‑100 (MQ=255)                                                     gttGTACGGGTTGTCGTGTCAGTGGTGCAAGAAATATAAAGACTGAGCATATCAACCAAGTGAAAAACACTATATTTATTGACGAGCGAAAAACCGATAc             >  1:6399890/2‑100 (MQ=255)                                                     cttGTACGGGTTGTCGTGTCAGTGGTGCAAGAAATATAAAGACTGAGCATATCAACCAAGTGAAAAACACTATATTTATTGACGAGCGAAAAACCGATAc             >  1:6164451/2‑100 (MQ=255)                                                       tGTACGGGTTGTCGTGTCAGTGGTGCAAGAAATATAAAGACTGAGCATATCAACCAAGTGAAAAACACTATATTTATTGACGAGCGAAAAACCGATACtt           >  1:1041619/1‑100 (MQ=255)                                                       tGTACGGGTTGTCGTGTCAGTGGTGCAAGAAATATAAAGACTGAGCATATCAACCAAGTGAAAAACACTATATTTATTGACGAGCGAAAAACCGATACtt           >  1:2781002/1‑100 (MQ=255)                                                            gggTTGTCGTGTCAGTGGTGCAAGAAATATAAAGACTGAGCATATCAACCAAGTGAAAAACACTATATTTATTGACGAGCGAAAAACCGATACTTCCCCt      <  1:2500465/100‑1 (MQ=255)                                                                tGTCGTGTCAGTGGTGCAAGAAATATAAAGACTGAGCATATCAACCAAGTGAAAAACACTATATTTATTGACGAGCGAAAAACCGATACTTCCCCTAGat  <  1:5244582/100‑1 (MQ=255)                                                                                               |                                                                     GATCACGTCAGAAAGAAGAGAACTCGTTCATCATTAGCGCTATTCATAATGATTTGTACGGGTTGTCGTGTCAGTGGTGCAAGAAATATAAAGATTGAGCATATCAACCAAGTGAAAAACACTATATTTATTGACGAGCGAAAAACCGATACTTCCCCTAGAT  >  NC_007793/2127134‑2127296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑