Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	415,546	G→A	A174T (GCA→ACA)	SAUSA300_RS01915 →	chromosome partitioning protein ParB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	415,546	0	G	A	100.0%	206.2 / NA	74	A174T (GCA→ACA)	SAUSA300_RS01915	chromosome partitioning protein ParB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (72/2);  total (72/2)

ATTGAGTAAGTCGCGCCCGTATATAGCGAATATGTTGAGGTTATTACATTTGCCGAAAAAGATTGCTGACATGGTAAAAGATGGGCGACTGACAAGTGCACATGGACGAACGTTATTGGCAATTAAAGATGAACAACAAATGCTTAGGTTAGCGAAACGGGTTGTTAAAGAAAAGTGGAGTGTCAGATATTTAG  >  NC_007793/415449‑415642                                                                                                  |                                                                                                 tttGAGTAAGTCGCGCCCGTATATAGCGAATATGTTGAGGTTATTACATTTGCCGAAAAAGATTGCTGACATGGTAAAAGATGGGCGACTGACAAGTAca                                                                                                >  1:2697761/2‑100 (MQ=255)      gTAAGTCGCGCCCGTATATAGCGAATATGTTGAGGTTATTACATTTGCCGAAAAAGATTGCTGACATGGTAAAAGATGGGCGACTGACAAGTATACATgg                                                                                           >  1:1052100/1‑100 (MQ=255)             gcgcCCGTATATAGCGAATATGTTGAGGTTATTACATTTGCCGAAAAAGATTGCTGACATGGTAAAAGATGGGCGACTGACAAGTACACATGGACGAACg                                                                                    >  1:2796898/1‑100 (MQ=255)              gtgccGTATATAGCGAATATGTTGAGGTTATTACATTTGCCGAAAAAGATTGCTGACATGGTAAAAGATGGGCGACTGACAAGTACACATGGACGAACGt                                                                                   >  1:3664741/4‑100 (MQ=255)                     atataGCGAATATGTTGAGGTTATTACATTTGCCGAAATAGATTGCTGACATGGCAAAAGATGGGCGACTGACAAGTACACATGGACGAACGTTATTGGc                                                                            >  1:6488317/1‑100 (MQ=255)                      tataGCGAATATGGTGAGGTTATTACATTTGCCGAAAAAGATTGCTGACATGGTAAAAGATGGGCGACTGACAAGTACACATGGACGAACGTTATTGGCa                                                                           >  1:6382907/1‑100 (MQ=255)                          gCGAATATGTTGAGGTTATTACATTTGCCGAAAAAGATTGCTGACATGGTAAAAGATGGGCGACTGACAAGTACACATGGACGAACGTTATTGGCAATTa                                                                       >  1:6334989/1‑100 (MQ=255)                           cGAATATGTTGAGGTTATTACATTTGCCGAAAAAGATTGCTGACATGGTAAAAGATGGGCGACTGACAAGTACACATGGACGAACGTTATTGGCAATTaa                                                                      <  1:5754736/100‑1 (MQ=255)                              atatGTTGAGGTTATTACATTTGCCGAAAAAGATTGCTGACATGGTAAAAGATGGGCGACTGACAAGTACACATGGACGAACGTTATTGGCAATTAAAGa                                                                   >  1:1830428/1‑100 (MQ=255)                                           attaCATTTGCCGAAAAAGATTGCTGACATGGTAAAAGATGGGCGACTGACAAGTACACATGGACGAACGTTATTGGCAATTAAAGATGAACAACAAATg                                                      >  1:3326987/1‑100 (MQ=255)                                                  ttGCCGAAAAAGATTGCTGACATGGTAAAAGATGGGCGACTGACAAGTACACATGGACGAACGTTATTGGCAATTAAAGATGAACAACAAATGCTTAGGt                                               >  1:6256689/1‑100 (MQ=255)                                                   tGCCGAAAAAGATTGCTGACATGGTAAAAGATGGGCGACTGACAAGTACACATGGACGAACGTTATTGGCAATTAAAGATGAACAACAAATGCTTAGGtt                                              <  1:2279736/100‑1 (MQ=255)                                                            aGATTGCTGACATGGTAAAAGATGGGCGACTGACAAGTACACATGGACGAACGTTATTGGCAATTAAAGATGAACAACAAATGCTTAGGTTAGCGAAACg                                     >  1:4256835/1‑100 (MQ=255)                                                              gttGCTGACATGGTAAAAGATGGGCGACTGACAAGTACACATGGACGAACGTTATTGGCAATTAAAGATGAACAACAAATGCTTAGGTTAGCGAAACggg                                   >  1:3148456/2‑100 (MQ=255)                                                               gtGCTGACATGGTAAAAGATGGGCGACTGACAAGTACACATGGACGAACGTTATTGGCAATTAAAGATGAACAACAAATGCTTAGGTTAGCGAAACGGgt                                  >  1:1116709/2‑100 (MQ=255)                                                                tGCTGACATGGTAAAAGATGGGCGACTGACAAGTACACATGGACGAACGTTATTGGCAATTAAAGATGAACAACAAATGCTTAGGTTAGCGAAACGGgtt                                 >  1:3465269/1‑100 (MQ=255)                                                                tGCTGACATGGTAAAAGATGGGCGACTGACAAGTACACATGGACGAACGTTATTGGCAATTAAAGATGAACAACAAATGCTTAGGTTAGCGAAACGGgtt                                 >  1:5860384/1‑100 (MQ=255)                                                                tGCTGACATGGTAAAAGATGGGCGACTGACAAGTACACATGGACGAACGTTATTGGCAATTAAAGATGAACAACAAATGCTTAGGTTAGCGAAACGGgtt                                 >  1:3154839/1‑100 (MQ=255)                                                                 gCTGACATGGTAAAAGATGGGCGACTGACAAGTACACATGGACGAACGTTATTGGCAATTAAGGATGAACAACAAATGCTTAGGTTAGCGAAACGGgttg                                >  1:2152908/1‑100 (MQ=255)                                                                 gCTGACATGGTAAAAGATGGGCGACTGACAAGTACACATGGACGAACGTTATTGGCAATTAAAGATGAACAACAAATGCTTAGGTTAGCGAAACGGgttg                                >  1:3231524/1‑100 (MQ=255)                                                                 gCTGACATGGTAAAAGATGGGCGACTGACAAGTACACATGGACGAACGTTATTGGCAATTAAAGATGAACAACAAATGCTTAGGTTAGCGAAACGGgttg                                >  1:1414761/1‑100 (MQ=255)                                                                 gCTGACATGGTAAAAGATGGGCGACTGACAAGTACACATGGACGAACGTTATTGGCAATTAAAGATGAACAACAAATGCTTAGGTTAGCGAAACGGgttg                                >  1:1167409/1‑100 (MQ=255)                                                                  cTGACATGGTAAAAGATGGGCGACTGACAAGTACACATGGACGAACGTTATTGGCAATTAAAGATGAACAACAAATGCTTAGGTTAGCGAAACGGgttgt                               >  1:981772/1‑100 (MQ=255)                                                                  cTGACATGGTAAAAGATGGGCGACTGACAAGTACACATGGACGAACGTTATTGGCAATTAAAGATGAACAACAAATGCTTAGGTTAGCGAAACGGgttgt                               >  1:2931920/1‑100 (MQ=255)                                                                  cTGACATGGTAAAAGATGGGCGACTGACAAGTACACATGGACGAACGTTATTGGCAATTAAAGATGAACAACAAATGCTTAGGTTAGCGAAACGGgttgt                               >  1:2428898/1‑100 (MQ=255)                                                                  cTGACATGGTAAAAGATGGGCGACTGACAAGTACACATGGACGAACGTTATTGGCAATTAAAGATGAACAACAAATGCTTAGGTTAGCGAAACGGgttgt                               >  1:2367419/1‑100 (MQ=255)                                                                    gACATGGTAAAAGATGGGCGACTGACAAGTACCCATGGACGAACGTTATTGGCAATTAAAGATGAACAACAAATGCTTAGGTTAGCGAAACGGGTTGTTa                             >  1:5380779/1‑100 (MQ=255)                                                                    gACATGGTAAAAGATGGGCGACTGACAAGTACACATGGACGAACGTTATTGGCAATTAAAGATGAACAACAAATGCTTAGGTTAGCGAAACGGgtagatc                             >  1:1397837/1‑95 (MQ=255)                                                                    gACATGGTAAAAGATGGGCGACTGACAAGTACACATGGACGAACGTTATTGGCAATTAAAGATGAACAACAAATGCTTAGGTTAGCGAAACGGGTTGTTa                             >  1:4986433/1‑100 (MQ=255)                                                                    gACATGGTAAAAGATGGGCGACTGACAAGTACACATGGACGAACGTTATTGGCAATTAAAGATGAACAACAAATGCTTAGGTTAGCGAAACGGGTTGTTa                             >  1:709901/1‑100 (MQ=255)                                                                    gACATGGTAAAAGATGGGCGACTGACAAGTACACATGGACGAACGTTATTGGCAATTAAAGATGAACAACAAATGCTTAGGTTAGCGAAACGGGTTGTTa                             >  1:6535213/1‑100 (MQ=255)                                                                    gACATGGTAAAAGATGGGCGACTGACAAGTACACATGGACGAACGTTATTGGCAATTAAAGATGAACAACAAATGCTTAGGTTAGCGAAACGGGTTGTTa                             >  1:1578085/1‑100 (MQ=255)                                                                    gACATGGTAAAAGATGGGCGACTGACAAGTACACATGGACGAACGTTATTGGCAATTAAAGATGAACAACAAATGCTTAGGTTAGCGAAACGGGTTGTTa                             >  1:5983508/1‑100 (MQ=255)                                                                    gACATGGTAAAAGATGGGCGACTGACAAGTACACATGGACGAACGTTATTGGCAATTAAAGATGAACAACAAATGCTTAGGTTAGCGAAACGGGTTGTTa                             >  1:5910179/1‑100 (MQ=255)                                                                    gACATGGTAAAAGATGGGCGACTGACAAGTACACATGGACGAACGTTATTGGCAATTAAAGATGAACAACAAATGCTTAGGTTAGCGAAACGGGTTGTTa                             >  1:2076683/1‑100 (MQ=255)                                                                    gACATGGTAAAAGATGGGCGACTGACAAGTACACATGGACGAACGTTATTGGCAATTAAAGATGAACAACAAATGCTTAGGTTAGCGAAACGGGTTGTTa                             >  1:5803509/1‑100 (MQ=255)                                                                    gACATGGTAAAAGATGGGCGACTGACAAGTACACATGGACGAACGTTATTGGCAATTAAAGATGAACAACAAATGCTTAGGTTAGCGAAACGGGTTGTTa                             >  1:5595112/1‑100 (MQ=255)                                                                    gACATGGTAAAAGATGGGCGACTGACAAGTACACATGGACGAACGTTATTGGCAATTAAAGATGAACAACAAATGCTTAGGTTAGCGAAACGGGTTGTTa                             >  1:5152891/1‑100 (MQ=255)                                                                    gACATGGTAAAAGATGGGCGACTGACAAGTACACATGGACGAACGTTATTGGCAATTAAAGATGAACAACAAATGCTTAGGTTAGCGAAACGGGTTGTTa                             >  1:4543190/1‑100 (MQ=255)                                                                    gACATGGTAAAAGATGGGCGACTGACAAGTACACATGGACGAACGTTATTGGCAATTAAAGATGAACAACAAATGCTTAGGTTAGCGAAACGGGTTGTTa                             >  1:4328541/1‑100 (MQ=255)                                                                    gACATGGTAAAAGATGGGCGACTGACAAGTACACATGGACGAACGTTATTGGCAATTAAAGATGAACAACAAATGCTTAGGTTAGCGAAACGGGGTGTTa                             >  1:6116487/1‑100 (MQ=255)                                                                    gACATGGTAAAAGATGGGCGACTGACAAGTACACATGGACGAACGTTATTGGCAATTAAAGATGAACAACAAATGCTTAGGTTAGCAAAAcagatcggaa                             >  1:1674455/1‑91 (MQ=255)                                                                    ctcATGGTAAAAGATGGGCGACTGACAAGTACACATGGACGAACGTTATTGGCAATTAAAGATGAACAACAAATGCTTAGGTTAGCGAAACGGGTTGTTa                             >  1:4591495/3‑100 (MQ=255)                                                                     ccATGGTAAAAGATGGGCGACTGACAAGTACACATGGACGAACGTTATTGGCAATTAAAGATGAACAACAAATGCTTAGGTTAGCGAAACGGGTTGTTaa                            >  1:4174233/2‑100 (MQ=255)                                                                        tGGTAAAAGATGGGCGACTGACAAGTACACATGGACGAACGTTATTGGCAATTAAAGATGAACAACAAATGCTTAGGTTAGCGAAACGGGTTGTTAAAGa                         >  1:4439476/1‑100 (MQ=255)                                                                         ggTAAAAGATGGGCGACTGACAAGTATACATGGACGAACGTTATTGGCAATTAAAGATGAACAACAAATGCTTAGGTTAGCGAAACGGGTTGTTAAAGaa                        >  1:2374247/1‑100 (MQ=255)                                                                         ggTAAAAGATGGGCGACTGACAAGTACACATGGACGAACGTTATTGGCAATTAAAGATGAACAACAAATGCTTAGGTTAGCGAAACGGGTTGTTAAAGaa                        >  1:658820/1‑100 (MQ=255)                                                                         ggTAAAAGATGGGCGACTGACAAGTACACATGGACGAACGTTATTGGCAATTAAAGATGAACAACAAATGCTTAGGTTAGCGAAACGGGTTGTTAAAGaa                        >  1:3101706/1‑100 (MQ=255)                                                                         ggTAAAAGATGGGCGACTGACAAGTACACATGGACGAACGTTATTGGCAATTAAAGATGAACAACAAATGCTTAGGTTAGCGAAACGGGTTGTTAAAGaa                        >  1:2639993/1‑100 (MQ=255)                                                                         ggTAAAAGATGGGCGACTGACAAGTACACATGGACGAACGTTATTGGCAATTAAAGATGAACAACAAATGCTTAGGTTAGCGAAACGGGTTGTTAAAGaa                        >  1:4844801/1‑100 (MQ=255)                                                                         ggTAAAAGATGGGCGACTGACAAGTACACATGGACGAACGTTATTGGCAATTAAAGATGAACAACAAATGCTTAGGTTAGCGAAACGGGTTGTTAAAGaa                        >  1:1853737/1‑100 (MQ=255)                                                                         ggTAAAAGATGGGCGACTGACAAGTACACATGGACGAACGTTATTGGCAATTAAAGATGAACAACAAATGCTTAGGTTAGCGAAACGGGTTGTTAAAGaa                        >  1:3641086/1‑100 (MQ=255)                                                                         cgTAAAAGATGGGCGACTGACAAGTACACATGGACGAACGTTATTGGCAATTAAAGATGAACAACAAATGCTTAGGTTAGCGAAACGGGTTGTTAAAGaa                        >  1:4908360/2‑100 (MQ=255)                                                                              aaGATGGGCGACTGACAAGTACACATGGACGAACGTTATTGGCAATTAAAGATGAACAACAAATGCTTAGGTTAGCGAAACGGGTTGTTAAAGAAAAGTg                   >  1:2407858/1‑100 (MQ=255)                                                                               ccaTGGGCGACTGACAAGTACACATGGACGAACGTTATTGGCAATTAAAGATGAACAACAAATGCTTAGGTTAGCGAAACGGGTTGTTAAAGAAAAGTgg                  >  1:2583119/3‑100 (MQ=255)                                                                               aactGGGCGACTGACAAGTACACATGGACGAACGTTATTGGCAATTAAAGATGAACAACAAATGCTTAGGTTAGCGAAACGGGTTGTTAAAGAAAAGTgg                  >  1:2997290/4‑100 (MQ=255)                                                                                 ttGGGCGACTGACAAGTACACATGGACGAACGTTATTGGCAATTAAAGATGAACAACAAATGCTTAGGTTAGCGAAACGGGTTGTTAAAGAAAAGTGGAg                >  1:6520008/2‑100 (MQ=255)                                                                                  tGGGCGACTGACAAGTACACATGGACGAACGTTATTGGCAATTAAAGATGAACAACAAATGCTTAGGTTAGCGAAACGGGTTGTTAAAGAAAAGTGGAgt               >  1:2266784/1‑100 (MQ=255)                                                                                   gggCGACTGACAAGTACACATGGACGAACGTTATTGGCAATTAAAGATGAACAACAAATGCTTAGGTTAGCGAAACGGGTTGTTAAAGAAAAGTGGAgtg              >  1:4728728/1‑100 (MQ=255)                                                                                     ccGACTGACAAGTACACATGGACGAACGTTATTGGCAATTAAAGATGAACAACAAATGCTTAGGTTAGCGAAACGGGTTGTTAAAGAAAAGTGGAGTGTc            >  1:5010793/2‑100 (MQ=255)                                                                                      acccTGACAAGTACACATGGACGAACGTTATTGGCAATTAAAGATGAACAACAAATGCTTAGGTTAGCGAAACGGGTTGTTAAAGAAAAGTGGAGTGTCa           >  1:3756931/4‑100 (MQ=255)                                                                                       gACTGACAAGTACACATGGACGAACGTTATTGGCAATTAAAGATGAACAACAAATGCTTAGGTTAGCGAAACGGGTTGTTAAAGACAAGTGGAGTGTCAg          >  1:4906429/1‑100 (MQ=255)                                                                                        ccTGACAAGTACACATGGACGAACGTTATTGGCAATTAAAGATGAACAACAAATGCTTAGGTTAGCGAAACGGGTTGTTAAAGAAAAGTGGAGTGTCAGa         >  1:6449768/2‑100 (MQ=255)                                                                                           gACAAGTACACATGGACGAACGTTATTGGCAATTAAAGATGAACAATAAATGCTTAGGTTAGCGAAACGGGTTGTTAAAGAAAAGAGGAGTGTCAGatat      >  1:3080507/1‑100 (MQ=255)                                                                                           gACAAGTACACATGGACGAACGTTATTGGCAATTAAAGATGAACAACAAATGCTTAGGTTAGCGAAACGGGTTGTTAAAGAAAAGTGGAgatcggaagag      >  1:6097148/1‑90 (MQ=255)                                                                                           gACAAGTACACATGGACGAACGTTATTGGCAATTAAAGATGAACAACAAATGCTTAGGTTAGCGAAACGGGTTGTTAAAGAAAAGTGGAGTGTCAGatat      >  1:1663248/1‑100 (MQ=255)                                                                                           gACAAGTACACATGGACGAACGTTATTGGCAATTAAAGATGAACAACAAATGCTTAGGTTAGCGAAACGGGTTGTTAAAGAAAAGTGGAGTGTCAGatat      >  1:3026873/1‑100 (MQ=255)                                                                                           gACAAGTACACATGGACGAACGTTATTGGCAATTAAAGATGAACAACAAATGCTTAGGTTAGCGAAACGGGTTGTTAAAGAAAAGTGGAGTGTCAGatat      >  1:865121/1‑100 (MQ=255)                                                                                           gACAAGTACACATGGACGAACGTTATTGGCAATTAAAGATGAACAACAAATGCTTAGGTTAGCGAAACGGGTTGTTAAAGAAAAGTGGAGTGTCAGatat      >  1:4524796/1‑100 (MQ=255)                                                                                           gACAAGTACACATGGACGAACGTTATTGGCAATTAAAGATGAACAACAAATGCTTAGGTTAGCGAAACGGGTTGTTAAAGAAAAGTGGAGTGTCAGatat      >  1:3198430/1‑100 (MQ=255)                                                                                           gACAAGTACACATGGACGAACGTTATTGGCAATTAAAGATGAACAACAAATGCTTAGGTTAGCGAAACGGGTTGTTAAAGAAAAGTGGAGTGTCAGatat      >  1:5792565/1‑100 (MQ=255)                                                                                            aCAGGTACACATGGACGAACGTTATTGGCAATTAAAGATGAACAACAAATGCTTAGGTTAGCGAAACGGGTTGTTAAAGAAAAGTGGAGTGTCAGATAtt     >  1:4119694/1‑100 (MQ=255)                                                                                               ctcgCACATGGACGCACGTTATTGGCAATTAAAGATGAACAACAAATGCTCAGGTTAGCGAAACGGGTTGTTAAAGAAAAGTGGAGTGTCAGATATTTAg  >  1:6390546/4‑100 (MQ=255)                                                                                               aGTACACATGGACGAACGTTATTGGCAATTAAAGATGAACAACAAATGCTTAGGTTAGCGAAACGGGTTGTTAAAGAAAAGTGGAGTGTCAGATATTTAg  >  1:4775942/1‑100 (MQ=255)                                                                                               aGTACACATGGACGAACGTTATTGGCAATTAAAGATGAACAACAAATGCTTAGGTTAGCGAAACGGGTTGTTAAAGAAAAGTGGAGTGTCAGATATTTAg  >  1:854469/1‑100 (MQ=255)                                                                                                  |                                                                                                 ATTGAGTAAGTCGCGCCCGTATATAGCGAATATGTTGAGGTTATTACATTTGCCGAAAAAGATTGCTGACATGGTAAAAGATGGGCGACTGACAAGTGCACATGGACGAACGTTATTGGCAATTAAAGATGAACAACAAATGCTTAGGTTAGCGAAACGGGTTGTTAAAGAAAAGTGGAGTGTCAGATATTTAG  >  NC_007793/415449‑415642

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑