Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,127,028	G→A	G139G (GGG→GGA)	SAUSA300_RS10835 →	integrase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,127,028	0	G	A	100.0%	82.3 / NA	32	G139G (GGG→GGA)	SAUSA300_RS10835	integrase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/1);  total (31/1)

AAACGATTATTCAAAAGAACACGCAAAAGAAACTATAAGAAAAACAAACGGTGCTATTCGGTCAGCTTTAGATGACGCATTATATGATGGGCTTATTTTTAAAAATCCCGCTTATAAAGTTAATTATAAAGCCGGAAAACCTACGAAGTCAGAACAAGAAAAATTCATCTCGGTAACTGAATATGA  >  NC_007793/2126938‑2127123                                                                                           |                                                                                                aaaCGACTATTCAAAAGAACACGCAAAAGAAACTATAAGAAAAACAAACGGTGCTATTCGGTCAGCTTTAGATGACGCATTATATGATGGACTTAttttt                                                                                        >  1:6067947/1‑100 (MQ=255)       ctattCAAAAGAACACGCAAAAGAAACTATAAGAAAAACAAACGGTGCTATTCGGTCAGCTTTAGATGACGCATTATATGATGGACTTATTTTTaaaaac                                                                                  >  1:1306150/2‑99 (MQ=255)                aGAACACGCAAAAGAAACTATAAGAAAAACAAACGGTGCTATTCGGTCAGCTTTAGATGACGCATTATATGATGGACTTATTTTTAAAAACCCCGCTtat                                                                         >  1:2694763/1‑100 (MQ=255)                 tcacacGCAAAAGAAACTATAAGAAAAACAAACGGTGCTATTCGGTCAGCTTTAGATGACGCATTATATGATGGACTTATTTTTAAAAACCCCGCTtata                                                                        >  1:875872/3‑100 (MQ=255)                  tacacGCAAAAGAAACTATAAGAAAAACAAACGGTGCTATTCGGTCAGCTTTAGATGACGCATTATATGATGGACTTATTTTTAAAAACCCCGCTTATaa                                                                       >  1:1872565/2‑100 (MQ=255)                   acacGCAAAAGAAACTATAAGAAAAACAAACGGTGCTATTCGGTCAGCTTTAGATGACGCATTATATGATGGACTTATTTTTAAAAACCCCGCTTATaaa                                                                      >  1:5695757/1‑100 (MQ=255)                   acacGCAAAAGAAACTATAAGAAAAACAAACGGTGCTATTCGGTCAGCTTTAGATGACGCATTATATGATGGACTTATTTTTAAAAACCCCGCTTATaaa                                                                      >  1:5593644/1‑100 (MQ=255)                   acacGCAAAAGAAACTATAAGAAAAACAAACGGTGCTATTCGGTCAGCTTTAGATGACGCATTATATGATGGACTTATTTTTAAAAACCCCGCTTATaaa                                                                      >  1:818838/1‑100 (MQ=255)                   acacGCAAAAGAAACTATAAGAAAAACAAACGGTGCTATTCGGTCAGCTTTAGATGACGCATTATATGATGGACTTATTTTTAAAAACCCCGCTTATaaa                                                                      >  1:5025640/1‑100 (MQ=255)                   acacGCAAAAGAAACTATAAGAAAAACAAACGGTGCTATTCGGACAGCTTTAGATGACGCATTATATGATGGACTTATTTTTAAAAACCCCGCTTATaaa                                                                      >  1:3773470/1‑100 (MQ=255)                    cacGCAAAAGAAACTATAAGAAAAACAAACGGCGCTATTCGGTCAGCTTTAGATGACGCATTATATGATGGACTTATTTTTAAAAACCCCGCTTATAAAg                                                                     >  1:6020773/1‑100 (MQ=255)                                   ataAGAAAAACAAACGGTGCTATTCGGTCAGCTTTAGATGACGCATTATATGATGGACTTATTTTTAAAAACCCCGCTTATAAAGTTAATTATAAAGCCg                                                      >  1:6542846/1‑100 (MQ=255)                                   ataAGAAAAACAAACGGTGCTATTCGGTCAGCTTTAGATGACGCATTATATGATGGACTTATTTTTAAAAACCCCGCTTATAAAGTTAATTATAAAGCCg                                                      >  1:5890806/1‑100 (MQ=255)                                       caaaaaCAAACGGTGCTATTCGGTCAGCTTTAGATGACGCATTATATGATGGACTTATTTTTAAAAACCCCGCTTATAAAGTTAATTATAAAGCCGGaaa                                                  >  1:2527072/2‑100 (MQ=255)                                        caccccaaacGGTGCTATCCGGTCAGCTTTAGATGACGCATTATATGCTGGACTTATTTTTAAAAACCCCGCTTATAAAGCTAATTATACAGCCGGaaaa                                                 >  1:4681261/6‑100 (MQ=255)                                         aaaaCAAACGGTGCTATTCGGTCAGCTTTAGATGACGCATTATATGATGGACTTATTTTTAAAAACCCCGCTTATAAAGTTAATTATAAAGCCGGAAAAc                                                >  1:5418968/1‑100 (MQ=255)                                          caacaaacGGTGCTATTCGGTCAGCTTTAGATGACGCATTATATGATGGACTTATTTTTAAAAACCCCGCTTATAAAGTTAATTATAAAGCCGGAAAAcc                                               >  1:6018470/2‑100 (MQ=255)                                               cccGGTGCTATTCGGTCAGCTTTAGATGACGCATTATATGATGGACTTATTTTTAAAAACCCCGCTTATAAAGTTAATTATAAAGCCGGAAAACCTACGa                                          >  1:3493175/3‑100 (MQ=255)                                                     gCTATTCGGTCAGCTTTAGATGACGCATTATATGATGGACTTATTTTTAAAAACCCCGCTTATAAAGTTAATTATAAAGCCGGAAAACCTACGAAGTCAg                                    >  1:182941/1‑100 (MQ=255)                                                     gCTATTCGGTCAGCTTTAGATGACGCATTATATGATGGACTTATTTTTAAAAACCCCGCTTATAAAGTTAATTATAAAGCCGGAAAACCTACGAAGTCAg                                    >  1:5825522/1‑100 (MQ=255)                                                           cGGTCAGCTTTAGATGACGCATTATATGATGGACTTATTTTTAAAAACCCCGCTTATAAAGTTAATTATAAAGCCGGAAAACCTACGAAGTCAGAACAAg                              >  1:6069921/1‑100 (MQ=255)                                                             ctCAGCTTTAGATGACGCATTATATGATGGACTTATTTTTAAAAACCCCGCTTATAAAGTTAATTATAAAGCCGGAAAACCTACGAAGTCAGAACAAGaa                            >  1:5393082/2‑100 (MQ=255)                                                               cAGCTTTAGATGACGCATTATATGATGGACTTATTTTTAAAAGCCCCGCTTATAAAGTTAATTATAAAGCCGGAAAACCTACGAAGTCAGAACAAGaaaa                          >  1:2108485/1‑100 (MQ=255)                                                               cAGCTTTAGATGACGCATTATATGATGGACTTATTTTTAAAAACCCCGCTTATAAAGTTAATTATAAAGCCGGAACACCTACGAAGTCAGAACAAGaaaa                          >  1:2873948/1‑100 (MQ=255)                                                               cAGCTTTAGATGACGCATTATATGATGGACTTATTTTTAAAAACCCCGCTTATAAAGTTAATTATAAAGCCGGAAAACCTACGAAGTCAGatcggaagag                          >  1:5594279/1‑91 (MQ=255)                                                               cAGCTTTAGATGACGCATTATATGATGGACTTATTTTTAAAAACCCCGCTTATAAAGTTAATTATAAAGCCGGAAAACCTACGAAGTCAGAACAAGaaaa                          >  1:1030052/1‑100 (MQ=255)                                                                    ttAGATGACGCATTATATGATGGACTTATTTTTAAAAACCCCGCTTATAAAGTTAATTATAAAGCCGGAAAACCTACGAAGTCAGAACAAGAAAAATTCa                     >  1:4771051/1‑100 (MQ=255)                                                                         tGACGCATTATATGATGGACTTATTTTTTAAAACCCCGCTTAAAAGGTTAATAAAAAACCCGCAAAACCTAGGAAGCCAGAACAAGAAAAAtcctcccgg                >  1:949455/1‑92 (MQ=255)                                                                                     tgatgGACTTATTTTTAAAAACCCCGCTTATAAAGTTAATTATAAAGCCGGAAAACCTACGAAGTCAGAACAAGAAAAATTCATCTCGGTAACTGAatat    <  1:2747062/100‑1 (MQ=255)                                                                                      gatgGACTTATTTTTAAAAACCCCGCTTATAAAGTTAATTATAAAGCCGGAAAACCTACGAAGTCAGAACAAGAAAAATTCATCTCGGTAACTGAATATg   >  1:4683988/1‑100 (MQ=255)                                                                                      gatgGACTTATTTTTAAAAACCCCGCTTATAAAGTTAATTATAAAGCCGGAAAACCTACGAAGTCAGAACAAGAAAAATTCATCTCGGTAACTGAATATg   >  1:296473/1‑100 (MQ=255)                                                                                       atgGACTTATTTTTAAAAACCCCGCTTATAAAGTTAATTATAAAGCCGGAAAACCTACGAAGTCAGAACAAGAAAAATTCATCTCGGTAACTGAATATGa  >  1:66770/1‑100 (MQ=255)                                                                                           |                                                                                                AAACGATTATTCAAAAGAACACGCAAAAGAAACTATAAGAAAAACAAACGGTGCTATTCGGTCAGCTTTAGATGACGCATTATATGATGGGCTTATTTTTAAAAATCCCGCTTATAAAGTTAATTATAAAGCCGGAAAACCTACGAAGTCAGAACAAGAAAAATTCATCTCGGTAACTGAATATGA  >  NC_007793/2126938‑2127123

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑