Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,126,944	T→C	D111D (GAT→GAC)	SAUSA300_RS10835 →	integrase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,126,944	0	T	C	100.0%	21.6 / NA	10	D111D (GAT→GAC)	SAUSA300_RS10835	integrase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (6/4);  total (6/4)

AATGCATTACAACATATACAAAAACATAAAATAGGTAAAATGGAGTTATCAAAGATAAATAGACAAGTTTATCAGAAATTCATAAACGATTATTCAAAAGAACACGCAAAAGAAACTATAAGAAAAACAAACGGTGCTATTCGGTCAGCTTTAGATGACGCATTATATGATGGGCTTATTTTT  >  NC_007793/2126855‑2127037                                                                                          |                                                                                              aaTGCATTACAACATATACAAAAACATAAAATAGGTAAAATGGAGTTATCAAAGATAAATAGACAAGTTTATCAGAAATTCATAAACGactagatcggaa                                                                                     >  1:6476549/1‑92 (MQ=255) aaTGCATTACAACATATACAAAAACATAAAATAGGTAAAATGGAGTTATCAAAGATAAATAGACAAGTTTATCAGAAATTCATAAACGACTATTCAAAAg                                                                                     >  1:3304145/1‑100 (MQ=255)       gtACAACATATACAAAAACATAAAATAGGTAAAATGGAGTTATCAAAGATAAATAGACAAGTTTATCAGAAATTCATAAACGACTATTCAAAAGAACACg                                                                               >  1:33772/2‑100 (MQ=255)          caacaTATACAAAAACATAAAATAGGTAAAATGGAGTTATCAAAGATAAATAGACAAGTTTATCAGAAATTCATAAACGACTATTCAAAAGAACACGCaa                                                                            >  1:27226/1‑100 (MQ=255)                                 aGGTAAAATGGAGTTATCAAAGATAAATAGACAAGTTTATCAGAAATTCATAAACGACTATTCAAAAGAACACGCAAAAGAAACTATAAGAAaaacaaac                                                     >  1:4084102/1‑100 (MQ=255)                                   gTAAAATGGAGTTATCAAAGATAAATAGACAAGTTTATCAGAAATTCATAAACGACTATTCAAAAGAACACGCAAAAGAAACTATAAGAAAAACAAACgg                                                   <  1:3282840/100‑1 (MQ=255)                                      aaaTGGAGTTATCAAAGATAAATAGACAAGTTTATCAGAAATTCATAAACGACTATTCAAAAGAACACGCAAAAGAAACTATAAGAAAAACAAACggcat                                                <  1:2809802/100‑4 (MQ=255)                                       aaTGGAGTTATCAAAGATAAATAGACAAGTTTATCAGAAATTCATAAACGACTATTCAAAAGAACACGCAAAAGAAACTATAAGAAAAACAAACggggtc                                               <  1:6012766/100‑5 (MQ=255)                                                     aGATAAATAGACAAGTTTATCAGAAATTCATAAACGACTATTCAAAAGAACACGCAAAAGAAACTATAAGAAAAACAAACGGTGCTATTCGGTCAGCttt                                 <  1:646376/100‑1 (MQ=255)                                                                         cAGAAATTCATAAACGACTATTCAAAAGAACACGCAAAAGAAACTATAAGAAAAACAAACGGTGCTATTCGGTCAGCTTTAGATGACGCATTATatgatg             >  1:809763/1‑100 (MQ=255)                                                                                    aaaCGACTATTCAAAAGAACACGCAAAAGAAACTATAAGAAAAACAAACGGTGCTATTCGGTCAGCTTTAGATGACGCATTATATGATGGACTTAttttt  >  1:6067947/1‑100 (MQ=255)                                                                                          |                                                                                              AATGCATTACAACATATACAAAAACATAAAATAGGTAAAATGGAGTTATCAAAGATAAATAGACAAGTTTATCAGAAATTCATAAACGATTATTCAAAAGAACACGCAAAAGAAACTATAAGAAAAACAAACGGTGCTATTCGGTCAGCTTTAGATGACGCATTATATGATGGGCTTATTTTT  >  NC_007793/2126855‑2127037

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑