Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,126,660	T→C	Y17H (TAT→CAT)	SAUSA300_RS10835 →	integrase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,126,660	0	T	C	100.0%	31.1 / NA	14	Y17H (TAT→CAT)	SAUSA300_RS10835	integrase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/4);  total (10/4)

TAGCCCGCCTACCCTTATTATTTTTTGCCAATTTTGAGGAGGGAACGCATGAAAACACGTTGTTACGATGGTAAAAAATGGCAATATGAATTTAAGTATGAAGGAAAAAGATACCGTAAGAAAGGTTTTAGAACAAAGCGTGAAGCTAATTCTGCTGGACTAGACAAGTTAAATGAGTT  >  NC_007793/2126564‑2126742                                                                                                 |                                                                                   tAGCCCGCCTACCCTTATTATTTTTTGCCAATTTTGAGGAGGGAACGCATGAAAACACGTTGTTACGATGGTAAAAAATGGCAATATGAATTTAAGCATg                                                                                 <  1:3742058/100‑1 (MQ=255)           aCCCTTATTATTTTTTGCCAATTTTGAGGAGGGAACGCATGAAAACACGTTGTTACGATGGTAAAAAATGGCAATATGAATTTAAGCATGAAGGAAAAAg                                                                       >  1:6372267/1‑100 (MQ=255)                   tattGTTTGCCAATTTTGAGGAGGGAACGCATGAAAACACGTTGTTACGATGGTAAAAAATGGCAATATGAATTTAAGCATGAAGGGAAAAGATACCGTa                                                               <  1:6178044/100‑1 (MQ=255)                             cAATTTTGAGGAGGGAACGCATGAAAACACGTTGTTACGATGGTAAAACATGGCAATATGAATTTAAGCATGAAGGAAAAAGATACCGTAAGAAAGGttt                                                     >  1:1477386/1‑100 (MQ=255)                                           ttaCGCATGAAAACACGTTGTTACGATGGTAAAAAATGGCAATATGAATTTAAGCATGAAGGAAAAAGATACCGTAAGAAAGGTTTTAGAACAAAGCGTg                                       >  1:2732410/3‑100 (MQ=255)                                             ctgCATGAAAACACGTTGTTACGATGGTAAAAAATGGCAATATGAATTTAAGCATGAAGGAAAAAGATACCGTAAGAAAGGTTTTAGAACAAAGCGTGaa                                     >  1:2023813/3‑100 (MQ=255)                                                           gttgtgACGATGGTAAAAAATGGCAAGATTAATTTAATCATGAAGGAAAAAGATACCGTAAGAAAGGTTTTAGATCAAAGCGTGAAGCTAATTCTGCTgg                       <  1:4649049/100‑1 (MQ=255)                                                              gttACGATGGTGAAAAATGGCAATATGAATTTAAGCATGAAGGAAAAAGATACCGTAAGAAAGGTTTTAGAACAAAGCGTGAAGCTAATTCTGCTGGACt                    <  1:4885561/100‑1 (MQ=255)                                                              cgcccGATGGTAAAAAATGGCAATATGTATTTAAGCATGAAGGAAAAAGATACCGTAAGAAAGGTTTTAGAACAAAGCGTGAAGCTAATTCTGCTGGACt                    >  1:5530440/5‑100 (MQ=255)                                                                  cGATGGTAAAAAATGGCAATATGAATTTAAGCATGAAGGAAAAAGATACCGTAAGAAAGGTTTTAGAACAAAGCGTGAAGCTAATTCTGCTGGACTAGAc                >  1:4800466/1‑100 (MQ=255)                                                                            acctGGCAATATGAATTTAAGCATGAAGGAAAAAGATACCGTAAGAAAGGTTTTAGAACAAAGCGTGAAGCTAATTCTGCTGGACTAGACAAGTTAAATg      >  1:4716370/4‑100 (MQ=255)                                                                               tGGCAATATGAATTTAAGCATGAAGGAAAAAGATACCGTAAGAAAGGTTTTAGAACAAAGCGTGAAGCTAATTCTGCTGGACTAGACAAGTTAAATGAGt   >  1:1617709/1‑100 (MQ=255)                                                                                ggCAATATGGATTTAAGCATGAAGGAAAAAGATACCGTAAGAAAGGTTTTAGAACAAAGCGTGAAGCTAATTCTGCTGGACTAGACAAGTTAAATGAGtt  >  1:932431/1‑100 (MQ=255)                                                                                ggCAATATGAATTTAAGCATGAAGGAAAAAGATACCGTAAGAAAGGTTTTAGAACAAAGCTTGAAGCTAATTCTGCTGGACTAGACAAGTTAAATGAGtt  >  1:3048488/1‑100 (MQ=255)                                                                                                 |                                                                                   TAGCCCGCCTACCCTTATTATTTTTTGCCAATTTTGAGGAGGGAACGCATGAAAACACGTTGTTACGATGGTAAAAAATGGCAATATGAATTTAAGTATGAAGGAAAAAGATACCGTAAGAAAGGTTTTAGAACAAAGCGTGAAGCTAATTCTGCTGGACTAGACAAGTTAAATGAGTT  >  NC_007793/2126564‑2126742

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑