Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	414,724	C→T	intergenic (‑361/‑303)	SAUSA300_RS01910 ← / → SAUSA300_RS01915	cystathionine gamma‑synthase/chromosome partitioning protein ParB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	414,724	0	C	T	100.0%	138.7 / NA	52	intergenic (‑361/‑303)	SAUSA300_RS01910/SAUSA300_RS01915	cystathionine gamma‑synthase/chromosome partitioning protein ParB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (1/51);  total (1/51)

ACTGTATCGCTATAACGGGCGAACCCGTAGACACCTCATATTGGCATCAACACTCCAAGGCCATTTTCAAACACGCTTTCAAAATCTTCTCTCAGCTACTAAAGACTCTCTGTATAAGCAGGGTGTGTTTTACTTTCCTCTTTATTGTGTTTACGTTTCATTAAACTGTTATA  >  NC_007793/414645‑414817                                                                                |                                                                                              aCTGTATCGCTATAACGGGCGAACCCGTAGACACCTCATATTGGCATCAACACTCCAAGGCCATTTTCAAACACGCTTTTAAAATCTTCTCTCAGctact                                                                           <  1:336142/100‑1 (MQ=255) aCTGTATCGCTATAACGGGCGAACCCGTAGACACCTCATATTGGCATCAACACTCCAAGGCCATTTTCAAACACGCTTTTAAAATCTTCTCTCAGctact                                                                           <  1:4180340/100‑1 (MQ=255)   tGTATCGCTATAACGGGCGAACCCGTAGACACCTCATATTGGCATCAACACTCCAAGGCCATTTTCAAACACGCTTTTAAAATCTTCTCTCAGCTACTaa                                                                         <  1:380674/100‑1 (MQ=255)   tGTATCGCTATAACGGGCGAACCCGTAGACACCTCATATTGGCATCAACACTCCAAGGCCATTTTCAAACACGCTTTTAAAATCTTCTCTCAGCTACTaa                                                                         <  1:1565654/100‑1 (MQ=255)   tGTATCGCTATAACGGGCGAACCCGTAGACACCTCATATTGGCATCAACACTCCAAGGCCATTTTCAAACACGCTTTTAAAATCTTCTCTCAGCTACTaa                                                                         <  1:2063235/100‑1 (MQ=255)   tGTATCGCTATAACGGGCGAACCCGTAGACACCTCATATTGGCATCAACACTCCAAGGCCATTTTCAAACACGCTTTTAAAATCTTCTCTCAGCTACTaa                                                                         <  1:5752614/100‑1 (MQ=255)   tGTATCGCTATAACGGGCGAACCCGTAGACACCTCATATTGGCATCAACACTCCAAGGCCATTTTCAAACACGCTTTTAAAATCTTCTCTCAGCTACTaa                                                                         <  1:6398850/100‑1 (MQ=255)    gTATCGCTATAACGGGCGAACCCGTAGACACCTCATATTGGCATCAACACTCCAAGGCCATTTTCAAACACGCTTTTAAAATCTTCTCTCAGCTACTaaa                                                                        <  1:1127710/100‑1 (MQ=255)              aaCGGGCGAACCCGTAGACACCTCATATTGGCATCAACACTCCAAGGCCATTTTCAAACACGCTTTTAAAATCTTCTCTCAGCTACTAAAGACTCTCTGt                                                              <  1:2959559/100‑1 (MQ=255)                 gggCGAACCCGTAGACACCTCATATTGGCATCAACACTCCAAGGCCATTTTCAAACACGCTTTTAAAATCTTCTCTCAGCTACTAAAGACTCTCTGtata                                                           <  1:2827591/100‑1 (MQ=255)                    cGAACCCGTAGACACCTCATATTGGCATCAACACTCCAAGGCCATTTTCAAACACGCTTTTAAAATCTTCTCTCAGCTACTAAAGACTCTCTGTATAAGc                                                        <  1:6013785/100‑1 (MQ=255)                       aCCCGTAGACACCTCATATTGGCATCAACACTCCAAGGCCATTTTCAAACACGCTTTTAAAATCTTCTCTCAGCTACTAAAGACTCTCTGTATAAGCAgc                                                     <  1:2191001/100‑2 (MQ=255)                        cccGTAGACACCTCATATTGGCATCAACACTCCAAGGCCATTTTCAAACACGCTTTTAAAATCTTCTCTCAGCTACTAAAGACTCTCTGTATAAGCAggg                                                    >  1:6195765/1‑100 (MQ=255)                                                cAACACTCCAAGGCCATTTTCAAACACGCTTTTAAAATCTTCTCTCAGCTACTAAAGACTCTCTGTATAAGCAGGGTGTGTTTTACTTTCCTCTTTATtg                            <  1:5724820/100‑1 (MQ=255)                                                    gatctaaGGCCATTTTCAAACACGCTTTTAAAATCTTCTCTCAGCTACTAAAGACTCTCTGTATAAGCAGGGTGTGTTTTACTTTCCTCTTTATTGTGtt                        <  1:5015488/95‑1 (MQ=255)                                                    acTCCAAGGCCATTTTCAAACACGCTTTTAAAATCTTCTCTCAGCTACTAAAGACTCTCTGTATAAGCAGGGTGTGTTTTACTTTCCTCTTTATTGTGtt                        <  1:638613/100‑1 (MQ=255)                                                     cTCCAAGGCCATTTTCAAACACGCTTTTAAAATCTTCTCTCAGCTACTAAAGTCTCTCTGTATAAGCAGGGTGTGTTTTACTTTCCTCTTTATTGTGttt                       <  1:1106714/100‑1 (MQ=255)                                                     cTCCAAGGCCATTTTCAAACACGCTTTTAAAATCTTCTCTCAGCTACTAAAGACTCTCTGTATAAGCAGGGTGTGTTTTACTTTCCTCTTTATTGTGttg                       <  1:78195/100‑2 (MQ=255)                                                            gCCATTTTCAAACACGCTTTTAAAATCTTCTCTCAGCTACTAAAGACTCTCTGTATAAGCAGGGTGTGTTTTACTTTCCTCTTTATTGTGTTTACGttgg                <  1:1015866/100‑3 (MQ=255)                                                               aTTTTCAAACACGCTTTTAAAATCTTCTCTCAGCTACTAAAGACTCTCTGTATAAGCAGGGTGTGTTTTACTTTCCTCTTTATTGTGTTTACGTTTCAtt             <  1:634806/100‑1 (MQ=255)                                                                 tttCAAACACGCTTTTAAAATCTTCTCTCAGCTACTAAAGACTCTCTGTATAAGCAGGGTGTGTTTTACTTTCCTCTTTATTGTGTTTACGTTTCagctt           <  1:4322903/100‑5 (MQ=255)                                                                          cGCTTTTAAAATCTTCTCTCAGCTTCTAAAGACTCTCTGTATAAGCAGGGTGTGTTTTACTTTCCTCTTTATTGTGTTTACGTTTCATTAAACTGTtata  <  1:1734952/100‑1 (MQ=255)                                                                          cGCTTTTAAAATCTTCTCTCAGCTACTAAAGACTCTCTGTATAAGCAGGGTGTGTTTTACTTTCCTCTTTATTGTGTTTACGTTTCATTAAACTGTtata  <  1:6072059/100‑1 (MQ=255)                                                                          cGCTTTTAAAATCTTCTCTCAGCTACTAAAGACTCTCTGTATAAGCAGGGTGTGTTTTACTTTCCTCTTTATTGTGTTTACGTTTCATTAAACTGTtata  <  1:5855369/100‑1 (MQ=255)                                                                          cGCTTTTAAAATCTTCTCTCAGCTACTAAAGACTCTCTGTATAAGCAGGGTGTGTTTTACTTTCCTCTTTATTGTGTTTACGTTTCATTAAACTGTtata  <  1:5840504/100‑1 (MQ=255)                                                                          cGCTTTTAAAATCTTCTCTCAGCTACTAAAGACTCTCTGTATAAGCAGGGTGTGTTTTACTTTCCTCTTTATTGTGTTTACGTTTCATTAAACTGTtata  <  1:6643959/100‑1 (MQ=255)                                                                          cGCTTTTAAAATCTTCTCTCAGCTACTAAAGACTCTCTGTATAAGCAGGGTGTGTTTTACTTTCCTCTTTATTGTGTTTACGTTTCATTAAACTGTtata  <  1:5447544/100‑1 (MQ=255)                                                                          cGCTTTTAAAATCTTCTCTCAGCTACTAAAGACTCTCTGTATAAGCAGGGTGTGTTTTACTTTCCTCTTTATTGTGTTTACGTTTCATTAAACTGTtata  <  1:5241545/100‑1 (MQ=255)                                                                          cGCTTTTAAAATCTTCTCTCAGCTACTAAAGACTCTCTGTATAAGCAGGGTGTGTTTTACTTTCCTCTTTATTGTGTTTACGTTTCATTAAACTGTtata  <  1:5060811/100‑1 (MQ=255)                                                                          cGCTTTTAAAATCTTCTCTCAGCTACTAAAGACTCTCTGTATAAGCAGGGTGTGTTTTACTTTCCTCTTTATTGTGTTTACGTTTCATTAAACTGTtata  <  1:989912/100‑1 (MQ=255)                                                                          cGCTTTTAAAATCTTCTCTCAGCTACTAAAGACTCTCTGTATAAGCAGGGTGTGTTTTACTTTCCTCTTTATTGTGTTTACGTTTCATTAAACTGTtata  <  1:2965779/100‑1 (MQ=255)                                                                          cGCTTTTAAAATCTTCTCTCAGCTACTAAAGACTCTCTGTATAAGCAGGGTGTGTTTTACTTTCCTCTTTATTGTGTTTACGTTTCATTAAACTGTtata  <  1:1408214/100‑1 (MQ=255)                                                                          cGCTTTTAAAATCTTCTCTCAGCTACTAAAGACTCTCTGTATAAGCAGGGTGTGTTTTACTTTCCTCTTTATTGTGTTTACGTTTCATTAAACTGTtata  <  1:1409745/100‑1 (MQ=255)                                                                          cGCTTTTAAAATCTTCTCTCAGCTACTAAAGACTCTCTGTATAAGCAGGGTGTGTTTTACTTTCCTCTTTATTGTGTTTACGTTTCATTAAACTGTtata  <  1:196309/100‑1 (MQ=255)                                                                          cGCTTTTAAAATCTTCTCTCAGCTACTAAAGACTCTCTGTATAAGCAGGGTGTGTTTTACTTTCCTCTTTATTGTGTTTACGTTTCATTAAACTGTtata  <  1:2219796/100‑1 (MQ=255)                                                                          cGCTTTTAAAATCTTCTCTCAGCTACTAAAGACTCTCTGTATAAGCAGGGTGTGTTTTACTTTCCTCTTTATTGTGTTTACGTTTCATTAAACTGTtata  <  1:2316602/100‑1 (MQ=255)                                                                          cGCTTTTAAAATCTTCTCTCAGCTACTAAAGACTCTCTGTATAAGCAGGGTGTGTTTTACTTTCCTCTTTATTGTGTTTACGTTTCATTAAACTGTtata  <  1:2943637/100‑1 (MQ=255)                                                                          cGCTTTTAAAATCTTCTCTCAGCTACTAAAGACTCTCTGTATAAGCAGGGTGTGTTTTACTTTCCTCTTTATTGTGTTTACGTTTCATTAAACTGTtata  <  1:493470/100‑1 (MQ=255)                                                                          cGCTTTTAAAATCTTCTCTCAGCTACTAAAGACTCTCTGTATAAGCAGGGTGTGTTTTACTTTCCTCTTTATTGTGTTTACGTTTCATTAAACTGTtata  <  1:3029512/100‑1 (MQ=255)                                                                          cGCTTTTAAAATCTTCTCTCAGCTACTAAAGACTCTCTGTATAAGCAGGGTGTGTTTTACTTTCCTCTTTATTGTGTTTACGTTTCATTAAACTGTtata  <  1:3150426/100‑1 (MQ=255)                                                                          cGCTTTTAAAATCTTCTCTCAGCTACTAAAGACTCTCTGTATAAGCAGGGTGTGTTTTACTTTCCTCTTTATTGTGTTTACGTTTCATTAAACTGTtata  <  1:3416507/100‑1 (MQ=255)                                                                          cGCTTTTAAAATCTTCTCTCAGCTACTAAAGACTCTCTGTATAAGCAGGGTGTGTTTTACTTTCCTCTTTATTGTGTTTACGTTTCATTAAACTGTtata  <  1:3449990/100‑1 (MQ=255)                                                                          cGCTTTTAAAATCTTCTCTCAGCTACTAAAGACTCTCTGTATAAGCAGGGTGTGTTTTACTTTCCTCTTTATTGTGTTTACGTTTCATTAAACTGTtata  <  1:3988168/100‑1 (MQ=255)                                                                          cGCTTTTAAAATCTTCTCTCAGCTACTAAAGACTCTCTGTATAAGCAGGGTGTGTTTTACTTTCCTCTTTATTGTGTTTACGTTTCATTAAACTGTtata  <  1:4065469/100‑1 (MQ=255)                                                                          cGCTTTTAAAATCTTCTCTCAGCTACTAAAGACTCTCTGTATAAGCAGGGTGTGTTTTACTTTCCTCTTTATTGTGTTTACGTTTCATTAAACTGTtata  <  1:4124629/100‑1 (MQ=255)                                                                          cGCTTTTAAAATCTTCTCTCAGCTACTAAAGACTCTCTGTATAAGCAGGGTGTGTTTTACTTTCCTCTTTATTGTGTTTACGTTTCATTAAACTGTtata  <  1:436398/100‑1 (MQ=255)                                                                          cGCTTTTAAAATCTTCTCTCAGCTACTAAAGACTCTCTGTATAAGCAGGGTGTGTTTTACTTTCCTCCTTATTGTGTTTACGTTTCATTAAACTGTtata  <  1:889072/100‑1 (MQ=255)                                                                          cGCTTTTAAAATCTTCTCTCAGCTACTAAAGACTCTCTGTATAAGCAGGGTGTGTTTTACTTCCCTCTTTATTGTGTTTACGTTTCATTAAACTGTtata  <  1:1937663/100‑1 (MQ=255)                                                                          cGCTTTTAAAATCTTCTCTCAGCTACTAAAGACTCTCTGTATAAGCAGGGTGTGTTGTACTTTCCTCTTTATTGTGTTTACGTTTCATTAAACTGTtata  <  1:5742077/100‑1 (MQ=255)                                                                          cGCTTTTAAAATCTTCTCTCAGCTACTAAAGACTCTCTGTATAAGCAGGGTGTGGTTTACTTTCCTCTTTATTGTGTTTACGTTTCATTAAACTGTtata  <  1:6069864/100‑1 (MQ=255)                                                                          cGCTTTTAAAATCTTCTCTCAGCTACTAAAGACTCTCTGTATAAGCAGGATGTGTTTTACTTTCCTCTTTATTGTGTTTACGTTTCATTAAACTGTtata  <  1:2837446/100‑1 (MQ=255)                                                                          cGCTTTTAAAATCCTCTCTCAGCTTCTAAAGACTCTCTGTATAAGCAGGGTGTGTTTTACTTTCCTCTTTAGTGAGTTTACGTTTCATTAACCTGTtata  <  1:5462399/100‑1 (MQ=255)                                                                                |                                                                                              ACTGTATCGCTATAACGGGCGAACCCGTAGACACCTCATATTGGCATCAACACTCCAAGGCCATTTTCAAACACGCTTTCAAAATCTTCTCTCAGCTACTAAAGACTCTCTGTATAAGCAGGGTGTGTTTTACTTTCCTCTTTATTGTGTTTACGTTTCATTAAACTGTTATA  >  NC_007793/414645‑414817

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑