Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,121,898	G→A	S114S (AGC→AGT)	SAUSA300_RS10785 ←	oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,121,898	0	G	A	100.0%	17.6 / NA	8	S114S (AGC→AGT)	SAUSA300_RS10785	oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (1/7);  total (1/7)

AATACAATTTTTGGTTTATCACGTTCAATCTTTGTTTCTAATTGATTGATTGTGTTGTTAGCAATTTTTAAAGCACGTTGCATAATCATTTCTGGGCTGTTCCATGCTTTTTCAACTTGGATGAAGTATTG  >  NC_007793/2121803‑2121933                                                                                                |                                    aaTACAATTTTTGGTTTATCACGTTCAATCTTTGTTTCTAATTGATTGATTGTGTTGTTAGCAATTTTTAAAGCACGTTGCATAATCATTTCTGGACTGt                                 <  1:5061033/100‑1 (MQ=255)    aCAATTTTTGGTTTATCACGTTCAATCTTTGTTTCTAATTGATTGATTGTGTTGTTAGCAATTTTTAAAGCACGTTGCATAATCATTTCTGGACTGTTca                              <  1:1398507/100‑2 (MQ=255)         ttttGGTTTATCACGTTCAATCTTTGTTTCTAATTGATTGATTGTGTTGTTAGCAATTTTTAAAGCACGTTGCATAATCATTTCTGGACTGTTCCGTgat                         <  1:3558460/100‑3 (MQ=255)           ttGGTTTATCACGTTCAATCTTTGTTTCTAATTGATTGATTGTGTTGTTAGCAATTTTTAAAGCACGTTGCATAATCATTTCTGGACTGTTCCATGCttt                       <  1:2546028/100‑1 (MQ=255)                     tcttccgaTCTTTGGGTCTAATTGATTGATTGTGTTGTTAGCAATTTTTAAAGCACGTTGCATAATCATTTCTGGACTGTTCCATGCTTTCTCTACTTgg             <  1:4951850/93‑1 (MQ=255)                     aCGTTCAATCTTTGGTTCTAATTGATTGATTGTGTTGTTAGCAATTTTTAAAGCACGTTGCATAATCATTTCTGGACTGTTCCATGCTTTCTCTACTTgg             <  1:4851159/100‑1 (MQ=255)                        ttCAATCTTTGTTTCTAATTGATTGATTGTGTTGTTAGCAATTTTTAAAGCACGTTGCATAATCATTTCTGGACTGTTCCATGCTTTCTCTACTTGGATg          <  1:5734950/100‑1 (MQ=255)                                ttGTTTCTAATTGATTGATTGTGTTGTTAGCAATTTTTAAAGCACGTTGCATAATCATTTCTGGACTGTTCCATGCTTTCTCTACTTGGATGAAATactc  >  1:3692854/1‑97 (MQ=255)                                                                                                |                                    AATACAATTTTTGGTTTATCACGTTCAATCTTTGTTTCTAATTGATTGATTGTGTTGTTAGCAATTTTTAAAGCACGTTGCATAATCATTTCTGGGCTGTTCCATGCTTTTTCAACTTGGATGAAGTATTG  >  NC_007793/2121803‑2121933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑