Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,121,729	C→T	V171I (GTA→ATA)	SAUSA300_RS10785 ←	oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,121,729	0	C	T	100.0%	26.5 / NA	11	V171I (GTA→ATA)	SAUSA300_RS10785	oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (1/10);  total (1/10)

TACTGTGTAGGCATGTTATAATCCACACCCTTGCGTTTAATAAGGAATCCGTTTTGACGTAACCACTCAAACAATCTGCGTTGCCCGATGTTTACACCGTTTTGTTTAATGATCTTTGCTAACTCTCCAACTAAAATTGATGTCTTAGTAGT  >  NC_007793/2121635‑2121786                                                                                               |                                                          tACTGTGTAGGCATGTTATAATCCACACCCTTGCGTTTAATAAGGAATCCGTTTTGACGTAACCACTCAAACAATCTGCGTTGCCCGATGTTTATACCGt                                                      <  1:4411668/100‑1 (MQ=255)  aCTGTGTAGGCATGTTATAATCCACACCCTTGCGTTTAATAAGGAATCCGTTTTGACGTAACCACTCAAACAATCTGCGTTGCCCGATGTTTATACCGtt                                                     >  1:1583655/1‑100 (MQ=255)   cTGTGTAGGCATGTTATAATCCACACCCTTGCGTTTAATAAGGAATCCGTTTTGACGTAACCACTCAAACAATCTGCGTTGCCCGATGTTTATACCGgtt                                                    <  1:2165522/100‑1 (MQ=255)         aGGCATGTTATAATCCACACCCTTGCGTTTAATAAGGAATCCGTTTTGACGTAACCACTCAAACAATCTGCGTTGCCCGATGTTTATACCGTTTTGTTTa                                              <  1:960603/100‑1 (MQ=255)                    gaTCTACACCCTTGCGTTTAATAAGGAATCCGTTTTGACGTAACCACTCAAACAATCTGCGTTGCCCGATGTTTATACCGTTTTGTTTAATGATCTTTGc                                   <  1:4275176/99‑1 (MQ=255)                     aTCCACACCCTTGCGTTTAATAAGGAATCCGTTTTGACGTAACCACTCAAACAATCTGCGTTGCCCGATGTTTATACCGTTTTGTTTAATGATCTTTGCt                                  <  1:2346654/100‑1 (MQ=255)                      tCCACACCCTTGCGTTTAATAAGGAATCCGTTTTGACGTAACCACTCCAACAATCTGCGTTGCCCGATGTTTATACCGTTTTGTTTAATGATCTTTGCTa                                 <  1:5740793/100‑1 (MQ=255)                      ccgatctccctGCGTTTAATAAGGAATCCGTTTTGACGTAACCACTCAAACAATCTGCGTTGCCCGATGTTTATACCGTTTTGTTTAATGATCTTTGCTa                                 <  1:5148726/90‑1 (MQ=255)                        cacaCCCTTGCGTTTAATAAGGAATCCGTTTTGACGTAACCACTCAAACAATCTGCGTTGCCCGATGTTTATACCGTTTTGTTTAATGATCTTTGCTaag                               <  1:6332216/100‑2 (MQ=255)                                           aGGAATCCGTTTTGACGTAACCACTCAAACAATCTGCGTTGCCCGATGTTTATACCGTTTTGTTTAATGATCTTTGCTAACTCTCCAACTAAAATTGATg            <  1:5571138/100‑1 (MQ=255)                                                     tttGACGTAACCACTCAAACAATCTGCGTTGCCCGATGTTTATACCGTTTTGTTTAATGATCTTTGCTAACTCTCCAACTAAAATTGATGTCTtagtagt  <  1:780939/100‑1 (MQ=255)                                                                                               |                                                          TACTGTGTAGGCATGTTATAATCCACACCCTTGCGTTTAATAAGGAATCCGTTTTGACGTAACCACTCAAACAATCTGCGTTGCCCGATGTTTACACCGTTTTGTTTAATGATCTTTGCTAACTCTCCAACTAAAATTGATGTCTTAGTAGT  >  NC_007793/2121635‑2121786

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑