Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,121,001	G→A	H3H (CAC→CAT)	SAUSA300_RS10775 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,121,001	0	G	A	100.0%	32.0 / NA	13	H3H (CAC→CAT)	SAUSA300_RS10775	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (1/12);  total (1/12)

TCCATACAGGGTCTAATGAATAACCAATTAGCTCATCATATTCATCTTTTGTGATAAGTACATGTGTTTCAGGTATTGGAACTGTTACGTTTAAAATGTGTGGCATTTCTATCTTTCCTTTCGTGTATAATGTTGTTATCTCCTAGTGAAAGGAGGTGATAAGTATGCA  >  NC_007793/2120904‑2121072                                                                                                  |                                                                        cccATACAGGGTCTAATGAATAACCAATTAGCTCATCATATTCATCTTTTGTGATAAGTACATGTGTTTCAGGTATTGGAACTGTTACGTTTAAAAtatg                                                                       >  1:4373019/2‑100 (MQ=255)   cATACAGGGTCTAATGAATAACCAATTAGCTCATCATATTCATCTTTTGTGATAAGTACATGTGTTTCAGGTATTGGAACTGTTACGTTTAAAAtatgtg                                                                     <  1:5090761/100‑1 (MQ=255)    aTACAGGGTCTAATGAATAACCAATTAGCTCATCATATTCATCTTTTGTGATAAGTACATGTGTTTCAGGTATTGGAACTGTTACGTTTAAAATATGTgg                                                                    <  1:578059/100‑1 (MQ=255)    aTACAGGGTCTAATGAATAACCAATTAGCTCATCATATTCATCTTTTGTGATAAGTACATGTGTTTCAGGTATTGGAACTGTTACGTTTAAAATATGTgg                                                                    <  1:6250774/100‑1 (MQ=255)     tACAGGGTCTAATGAATAACCAATTAGCTCATCATATTCATCTTTTGTGATAAGTACATGTGTTTCAGGTATTGGAACTGTTACGTTTAAAGTATGTGGc                                                                   <  1:751794/100‑1 (MQ=255)     tACAGGGTCTAATGAATAACCAATTAGCTCATCATATTCATCTTTTGTGATAAGTACATGTGTTTCAGGTATTGGAACTGTTACGTTTAAAATATGTGGc                                                                   <  1:1223733/100‑1 (MQ=255)         gggTCTAATGAATAACCAATTAGCTCATCATATTCATCTTTTGTGATAAGTACATGTGTTTCAGGTATTGGAACTGTTACGTTTAAAATATGTGGCAtgg                                                               <  1:1265304/100‑3 (MQ=255)                              aGCTCATCATATTCATCTTTTGTGATAAGTACATGTGTTTCAGGTATTGGAACTGTTACGTTTAAAATATGTGGCATTTCTATCTTTCCTTTCGTGtata                                          <  1:2143620/100‑1 (MQ=255)                                     tcttccgaTCTTGTGTGATAAGTACATGTGTTTCAGGTATTGGAACTGTTACGTTTAAAATATGTGGCATTTCTATCTTTCCTTTCGTGTATAAtgttgt                                   <  1:559213/93‑1 (MQ=255)                                     catATTCATCTTTTGTGATAAGTACATGTGTTTCAGGTATTGGAACTGTTACGTTTAAAATATGTGGCATTTCTATCTTTCCTTTCGTGTATAAtgttgt                                   <  1:153829/100‑1 (MQ=255)                                      atatTCATCTTTTGTGATAAGTACATGTGTTTCAGGTATTGGAACTGTTACGTTTAAAATATGTGGCATTTCTATCTTTCCTTTCGTGTATAATgttgtt                                  <  1:4659508/100‑1 (MQ=255)                                                tttGTGATAAGTACATGTGTTTCAGGTATTGGAACTGTTACGTTTAAAATATGTGGCATTTCTATCTTTCCTTTCGTGTATAATGTTGTTATCTCCTAgc                        <  1:130150/100‑2 (MQ=255)                                                           tACATGTGTTTCAGGTATTGGAACTGTTACGTTTAAAATATGTGGCATTTCTATCTTTCCTTTCGTGTATAATGTTGTTATCTCCTAGTGAAAGGAGGTg             <  1:5696679/100‑1 (MQ=255)                                                                     tCAGGTATTGGAACTGTTACGTTTAAAATATGTGGCATTTCTATCTTTCCTTTCGTGTATAATGTTGTTATCTCCTAGTGAAAGGGGGTGATattggtgt   >  1:4969213/1‑93 (MQ=255)                                                                      cAGGTATTGGAACTGTTACGTTTAAAATATGTGGCATTTCTATCTTTCCTTTCGTGTATAATGTTGTTATCTCCTAGTGAAAGGAGGTGATattggtgta  <  1:6585295/100‑9 (MQ=255)                                                                                                  |                                                                        TCCATACAGGGTCTAATGAATAACCAATTAGCTCATCATATTCATCTTTTGTGATAAGTACATGTGTTTCAGGTATTGGAACTGTTACGTTTAAAATGTGTGGCATTTCTATCTTTCCTTTCGTGTATAATGTTGTTATCTCCTAGTGAAAGGAGGTGATAAGTATGCA  >  NC_007793/2120904‑2121072

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑