Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,120,777	C→T	S78N (AGT→AAT)	SAUSA300_RS10775 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,120,777	0	C	T	100.0%	106.2 / NA	40	S78N (AGT→AAT)	SAUSA300_RS10775	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (2/38);  total (2/38)

CATTTTATTCTCTCCTTATATATTTCATTGAAATGCTCATCGACGAATTTATTCATCTTTCTTGCGTTAAATCTCCAGCGATTAAAACTCTCATCTGGGTAATGCACAATTCCTTGCGCTCTTAGTTCTTTTTCAAATCTAGGATGAAATAGTAATCTGTCCTTGATAGTCTCATCAGATGCAA  >  NC_007793/2120690‑2120873                                                                                        |                                                                                                 ttccgatctctctcCTTATATATTTCATTGAAATGCTCATCGACGAATTTATTCATCTTTCTTGCGTTAAATCTCCAGCGATTAAAATTCTCATCAGGGt                                                                                      <  1:13421/92‑1 (MQ=255) gctcttccgatctcCTTCTATATTTCATTGAAATGCTCATCGACGAATTTATTCATCTTTCTTGCGTTAAATCTCCAGCGATTAAAATTCTCATCAGGGt                                                                                      <  1:6225368/90‑1 (MQ=255) gctcttccgatctcCTTATATATTTCATTGAAATGCTCATCGACGAATTTATTCATCTTTCTTGCGTTAAATCTCCAGCGATTAAAATTCTCATCAGGGt                                                                                      <  1:3240614/90‑1 (MQ=255)  tccgatctctctgCTTATATATTTCATTGAAATGCTCATCGACGAATTTATTCATCTTTCTTGCGTTAAATCTCCAGCGATTAAAATTCTCATCAGGGTa                                                                                     <  1:848885/93‑1 (MQ=255)  tccgatctctctcCTTATATATTTCATTGAAATGCTCAGCGACGAATTTATTCATCTTTCTTGCGTTAAATCTCCAGCGATTAAAATTCTCATCATGGTa                                                                                     <  1:6020297/93‑1 (MQ=255)  cttccgatctcttCTCATATATTTCATTGAAATGCTCATCGACGAATTTATTCATCTTTCTTGCGTTAAATCTCCAGCGATTAAAATTCTCATCAGGGTa                                                                                     <  1:4653960/93‑1 (MQ=255)    tccgatctcttCGTATATATTACATTAAAATGCTCATCGACGAATTTATTCATCTTTCTTGCGTTAAATCTCCATCGATTAAAATTCTCATCAGGGTAAt                                                                                   <  1:4355244/95‑1 (MQ=255)    tccgatctctcCTTATATATTTCATTGAAATGCTCATCGACGAATTTATTCATCTTTCTTGCGTTAAATCTCCAGCGATTAAAATTCTCATCAGGGTaca                                                                                   <  1:4011771/95‑3 (MQ=255)    tccgatctctcCTTATATATTTCATTGAAATGCTCATCGACGAATTTATTCATCTTTCTTGCGTTAAATCTCCAGCGATTAAAATTCTCATCAGGGTaac                                                                                   <  1:5345286/95‑2 (MQ=255)    tccgatctctcCTCATATATTTCATAGAAATGCTCATCGGCGAATTTATTCATCTTTCTTGCTTTAAATCTCCAGCGATTAAAATTCTCATAAGGGTact                                                                                   <  1:4577344/95‑3 (MQ=255)        atctctcTTTATATATTTCATTGAAATGCTCATCGACGAATTTATTCATCTTTCTTGCGTTAAATCTCCAGCGATTAAAATTCTCATCAGGGTAATGcac                                                                               <  1:4530824/99‑1 (MQ=255)              ccTTATATATTTCATTGAAATGCTCATCGACGAATTTATTCATCTTTCTTGCGTTAAATCTCCAGCGATTAAAATTCTCATCAGGGTAATGCACAATTcc                                                                         >  1:5157602/1‑100 (MQ=255)                    atatTTCATTGAAATGCTCATCGACGAATTTATTCATCTTTCTTGCGTTAAATCTCCAGCGATTAAAATTCTCATCAGGGTAATGCACAATTCCTTgcgg                                                                   <  1:944755/100‑2 (MQ=255)                        ttCATTGAAATGCTCATCGACGAATTTATTCATCTTTCTTGCGTTAAATCTCCAGCGATTAAAATTCTCATCAGGGTAATGCACAATTCCTTGCGCTCtt                                                               <  1:3484043/100‑1 (MQ=255)                        ttCATTGAAATGCTCATCGACGAATTTATTCATCTTTCTTGCGTTAAATCTCCAGCGATTAAAATTCTCATCAGGGTAATGCACAATTCCTTGCGCTCtt                                                               <  1:4057437/100‑1 (MQ=255)                        ttCATTGAAATGCTCATCGACGAATTTATTCATCTTTCTTGCGTTAAATCTCCAGCGATTAAAATTCTCATCAGGGTAATGCACAATTCCTTGCGCTCtt                                                               <  1:5785215/100‑1 (MQ=255)                           aTTGAAATGCTCATCGACGAATTTATTCATCTTTCTTGCGTTAAATCTCCAGCGATTAAAATTCTCATCAGGGTAATGCACAATTCCTTGCGCTCTTAGt                                                            <  1:1364826/100‑1 (MQ=255)                            ttGAAATGCTCATCGACGAATTTATTCATCTTTCTTGCGTTAAATCTCCAGCGATTAAAATTCTCATCAGGGTAATGCACAATTCCTTGCGCTCTTAggc                                                           <  1:3455059/100‑3 (MQ=255)                                aaTGCTCATCGACGAATTTATTCATCTTTCTTGCGTTAAATCTCCAGCGATTAAAATTCTCATCAGGGTAATGCACAATTCCTTGCGCTCTTAggcagtt                                                       <  1:3188858/100‑7 (MQ=255)                                aaTGCTCATCGACGAATTTATTCATCTTTCTTGCGTTAAATCTCCAGCGATTAAAATTCTCATCAGGGTAATGCACAATTCCTTGCGCTCTTAGTTCtca                                                       <  1:2597549/100‑3 (MQ=255)                                               aTTTATTCATCTTTCTTGCGTTAAATCTCCAGCGATTAAAATTCTCATCAGGGTAATGCACAATTCCTTGCGCTCTTAGTTCTTTTTCAAATCTAGGATg                                        <  1:2334221/100‑1 (MQ=255)                                                tttATTCATCTTTCTTGCGTTAAATCTCCAGCGATTAAAATTCTCATCAGGGTAATGCACAATTCCTTGCGCTCTTAGTTCTTTTTCAAATCTAGGATGa                                       <  1:4739231/100‑1 (MQ=255)                                                                    tAAATCTCCAGCGATTAAAATTCTCATCAGGGTAATGCACAATTCCTTGCGCTGTTAGTTCTTTTTCAAATCTAGGATGAAATAGTAATCTGTCCTTGAt                   <  1:4481330/100‑1 (MQ=255)                                                                    tAAATCTCCAGCGATTAAAATTCTCATCAGGGTAATGCACAATTCCTTGCGCTCTTAGTTCTTTTTCAAATCTAGGATGAAATAGTAATCTGTCCTTGAt                   <  1:1539648/100‑1 (MQ=255)                                                                    ccgaTCTCCAGCGATTAAAATTCTCATCAGGGTAATGCACAATTCCTTGCGCTCTTAGTTCTTTTTCAAATCTAGGATGAAATAGTAATCTGTCCTTGAt                   <  1:456415/97‑1 (MQ=255)                                                                         ctcCAGCGATTAAAATTCTCATCAGGGTAATGCACAATTCCTTGCGCTCTTAGTTCTTTTTCAAATCTAGGATGAAATAGTAATCTGTCCTTGATAGtct              >  1:3973941/1‑100 (MQ=255)                                                                          tcCAGCGATTAAAATTCTCATCAGGGTAATGCACAATTCCTTGCGCACTTAGTTCTTTTTCAAATCTAGGATGAAATAGTAATCTGTCCTTGATAGtctc             <  1:2269052/100‑1 (MQ=255)                                                                            cAGCGATTAAAATTCTCATCAGGGTAATGCACAATTCCTTGCGCTCTTAGTTCTTTTTCAAATCTAGGATGAAATAGTAATCTGTCCTTGATAGTCtcat           <  1:2174417/100‑1 (MQ=255)                                                                             tgCGATTAAAATTCTCATCAGGGTAATGCACAATTCCTTGCGCTCTTAGTTCTTTTTCAAATCTAGGATGAAATAGTAATCTGTCCTTGATAGTCtcatc          <  1:2863936/99‑1 (MQ=255)                                                                             aGCGATTAAAATTCTCATCAGGGTAATGCACAATTCCTTGCGCTCTTAGTTCTTTTTCAAATCTAGGATGAAATAGTAATCTGTCCTTGATAGTCtcatc          <  1:6642643/100‑1 (MQ=255)                                                                             aGCGATTAAAATTCTCATCAGGGTAATGCACAATTCCTTGCGCTCTTAGTTCTTTTTCAAATCTAGGATGAAATAGTAATCTGTCCTTGATAGTCtcatc          <  1:667920/100‑1 (MQ=255)                                                                             aGCGATTAAAATTCTCATCAGGGTAATGCACAATTCCTTGCGCTCTTAGTTCTTTTTCAAATCTAGGATGAAATAGTAATCTGTCCTTGATAGTCtcatc          <  1:11135/100‑1 (MQ=255)                                                                             aGCGATTAAAATTCTCATCAGGGTAATGCACAATTCCTTGCGCTCTTAGTTCTTTTTCAAATCTAGGATGAAATAGTAATCTGTCCTTGATAGTCtcatc          <  1:6062917/100‑1 (MQ=255)                                                                             aGCGATTAAAATTCTCATCAGGGTAATGCACAATTCCTTGCGCTCTTAGTTCTTTTTCAAATCTAGGATGAAATAGTAATCTGTCCTTGATAGTCtcatc          <  1:6004537/100‑1 (MQ=255)                                                                             aGCGATTAAAATTCTCATCAGGGTAATGCACAATTCCTTGCGCTCTTAGTTCTTTTTCAAATCTAGGATGAAATAGTAATCTGTCCTTGATAGTCtcatc          <  1:2308716/100‑1 (MQ=255)                                                                             aGCGATTAAAATTCTCATCAGGGTAATGCACAATTCCTTGCGCTCTTAGTTCTTTTTCAAATCTAGGATGAAATAGTAATCTGTCCTTGATAGTCtcatc          <  1:2841373/100‑1 (MQ=255)                                                                             aGCGATTAAAATTCTCATCAGGGTAATGCACAATTCCTTGCGCTCTTAGTTCTTTTTCAAATCTAGGATGAAATAGTAATCTGTCCTTGATAGTCtcatc          <  1:3308867/100‑1 (MQ=255)                                                                                   tAAAATTCTCATCAGGGTAATGCACAATTCCTTGCGCTCTTAGTTCTTTTTCAAATCTAGGATGAAATAGTAATCTGTCCTTGATAGTCTCATCAGATGc    <  1:260057/100‑1 (MQ=255)                                                                                   tAAAATTCTCATCAGGGTAATGCACAATTCCTTGCGCTCTTAGTTCTTTTTCAAATCTAGGATGAAATAGTAATCTGTCCTTGATAGTCTCATCAGATGc    <  1:708480/100‑1 (MQ=255)                                                                                     aaaTTCTCATCAGGGTAATGCACAATTCCTTGCGCTCTTAGTTCTTTTTCAAATCTAGGATGAAATAGTAATCTGTCCTTGATAGTCTCATCAGATGCaa  <  1:2117662/100‑1 (MQ=255)                                                                                        |                                                                                                 CATTTTATTCTCTCCTTATATATTTCATTGAAATGCTCATCGACGAATTTATTCATCTTTCTTGCGTTAAATCTCCAGCGATTAAAACTCTCATCTGGGTAATGCACAATTCCTTGCGCTCTTAGTTCTTTTTCAAATCTAGGATGAAATAGTAATCTGTCCTTGATAGTCTCATCAGATGCAA  >  NC_007793/2120690‑2120873

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑