Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,120,686	C→T	D3N (GAC→AAC)	SAUSA300_RS10770 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,120,686	0	C	T	100.0%	14.5 / NA	7	D3N (GAC→AAC)	SAUSA300_RS10770	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/1);  total (6/1)

CCACGCTGTAGTGAAGTACAGCAATGGCATAAGCACAATCGCTAAGACTGTGAAACATAGCACTGCTAATAGGTAGCTTTTATATGTGTCGCTCATTTTATTCTCTCCTTATATATTTCATTGAAATGCTCATCGACGAATTTATTCATCTTTCTTGCGTTAAATCTCCA  >  NC_007793/2120597‑2120766                                                                                          |                                                                                 ccATGCAGTAGTGAAGTATAGAAACGGCATAAGTACAATTGCTAAGACTGTAAAGCACAGTACTGCTACTAGGTAGCTTTTATAAATGTTGCTCATTTTa                                                                        >  1:3082779/1‑100 (MQ=255) ccATGCAGTAGTGAAGTATAGAAACGGCATAAGTACAATTGCTAAGACTGTAAAGCACAGTACTGCTACTAGGTAGCTTTTATAAATGTTGCTCATTTTa                                                                        >  1:390789/1‑100 (MQ=255)                                         gCTAAGACTGTAAAGCACAGTACTGCTACTAGGTAGCTTTTATAAATGTTGCTCATTTTATTCTCTCCTTATATATTTCATTGAAATGCTCATCGACGaa                                >  1:1255198/1‑100 (MQ=25)                                         gCTAAGACTGTAAAGCACAGTACTGCTACTAGGTAGCTTTTATAAATGTTGCTCATTTTATTCTCTCCTTATATATTTCATTGAAATGCTCATCGACGaa                                >  1:5866592/1‑100 (MQ=25)                                                       ctgcagtaCTGCTACTAGGTAGCTTTTATAAATGTTGCTCATTTTATTCTCTCCTTATATATTTCATTGAAATGCTCATCGACGAATTTATTCAtctttc                  >  1:5309111/8‑100 (MQ=255)                                                              aCTGCTACTAGGTAGCTTTTATAAATGTTGCTCATTTTATTCTCTCCTTATATATTTCATTGAAATGCTCATCGACGAATTTATTCATCTTTCTTGCGtt           <  1:101600/100‑1 (MQ=255)                                                                       aGGTAGCTTTTATAAATGTTGCTCATTTTATTCTCTCCTTATATATTTCATTGAAATGCTCATCGACGAATTTATTCATCTTTCTTGCGTTAAATCTCCa  >  1:4994069/1‑100 (MQ=255)                                                                                          |                                                                                 CCACGCTGTAGTGAAGTACAGCAATGGCATAAGCACAATCGCTAAGACTGTGAAACATAGCACTGCTAATAGGTAGCTTTTATATGTGTCGCTCATTTTATTCTCTCCTTATATATTTCATTGAAATGCTCATCGACGAATTTATTCATCTTTCTTGCGTTAAATCTCCA  >  NC_007793/2120597‑2120766

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑