Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	407,945	C→T	P27P (CCG→CCA)	SAUSA300_RS01890 ←	metal‑dependent hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	407,945	0	C	T	100.0%	90.2 / NA	35	P27P (CCG→CCA)	SAUSA300_RS01890	metal‑dependent hydrolase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (0/35);  total (0/35)

CAATATTCCAACGTTGCACATAGAAACCATGATCTTTAACCGTGAATAAAGTTGAAACTTCGCCTTTTTCAAACTCACTAAAACGTGGTATTTCCGGATCAAATGTATGCGTTAAATCAACCCAAGTTGCTTGTTTTAAAGTATTTAATTGTTGCCATAAAGGATATTGTGTCATAAAATCACCCG  >  NC_007793/407851‑408036                                                                                               |                                                                                            cAATATTCCAACGTTGCACATAGAAACCATGATCTTTAACCGTGAATAAAGTTGAAACTTCGCCTTTTTCAAACTCACTAAAACGTGGTATTTCTGGATc                                                                                        <  1:5912173/100‑1 (MQ=255)  cttccgatctcGTTGCACATAGAAACCATGATCTTTAACCGTGAATAAAGTTGAAACTTCGCCTTTTTCAAACTCACTAAAACGTGGTATTTCTGGATCa                                                                                       <  1:2914658/90‑1 (MQ=255)           aCGTTGCACATAGAAACCATGATCTTTAACCGTGAATAAAGTTGAAACTTCGCCTTTTTCAAACTCACTAAAACGTGGTATTTCTGGATCAAATGTATGc                                                                              <  1:1462616/100‑1 (MQ=255)                        ctctgccgATCTTTAGCCGTGAATAAAGTTGAAACTTCGCCTTTTTCAAACTCACTAAAACGTGGTATTTCTGGATCAAATGTATGCGTTAAATCAAccc                                                                 <  1:6055045/93‑1 (MQ=255)                          cttccgATCTTTAATCGTGAATAAAGTTGAAACTTCGCCTTTTTCAAACTCACTAAAACGTGGTATTTCTGGATCAAATGTATGCGTTAAATCAACCCaa                                                               <  1:4936968/95‑1 (MQ=255)                          cttccgATCTTT‑ACCGTGAATAAAGTTGAAACTTCGCCTTTTTCAAACTCACTAAAACGTGGTATTTCTGGATCAAATGTATGCGTTAAATCAACCCAAg                                                              <  1:6567664/95‑1 (MQ=255)                            tccgATCTTTTGCCGTGAATAAAGTTGAAACTTCGCCTTTTTCAAACTCACTAAAACGTGGTATTTCTGGATCAAATGTATGCGTTAAATCAACCCAAGt                                                             <  1:2919307/97‑1 (MQ=255)                                  tctgtgccGTGAATAAAGTTGAAACTTCGCCTTTTTCAAACTCACTAAAACGTGGTATTTCTGGATCAAATGTATGCGTTAAATCAACCCAAGTTGCTTg                                                       <  1:4375981/94‑1 (MQ=255)                                      aaCCGTGAATAAAGTTGAAACTTCGCCTTTTTCAAACTCACTAAAACGTGGTATTTCTGGATCAAATGTATGCGTTAAATCAACCCAAGTTGCTTGtttt                                                   <  1:3466398/100‑1 (MQ=255)                                             aaTAAAGTTGAAACTTCGCCTTTTTCAAACTCACTAAAACGTGGTATTTCTGGATCAAATGTATGCGTTAAATCAACCCAAGTTGCTTGTTTTAAAGTAt                                            <  1:6583470/100‑1 (MQ=255)                                                        aaCTTCGCCTTTTTCAAACTCACTAAAACGTGGTATTTCTGGATCAAATGTATGCGTTAAATCAACCCAAGTTGCTTGTTTTAAAGTATTTAATTGTTGc                                 <  1:5134389/100‑1 (MQ=255)                                                        aaCTTCGCCTTTTTCAAACTCACTAAAACGTGGTATTTCTGGATCAAATGTATGCGTTAAATCAACCCAAGTTGCTTGTTTTAAAGTATTTAATTGTTGc                                 <  1:3285793/100‑1 (MQ=255)                                                          cTTCGCCTTTTTCAAACTCACTAAAACGTGGTATTTCTGGATCAAATGTATGCGTTAAATCAACCCAAGTTGCTTGTTTTAAAGTATTTAATTGTTGCCa                               <  1:5989926/100‑1 (MQ=255)                                                           ctCGCCTTTTTCAAACTCACTAAAACGTGGTATTTCTGGATCAAATGTATGCGTTAAATCAACCCAAGTTGCTTGTTTTAAACTATTTAATTGTTTCCAt                              <  1:1410789/99‑1 (MQ=255)                                                             tgCCTTTTTCAAACTCACTAAAACGTGGTATTTCTGGATCAAATGTATGCGTTAAATCAACCCAAGTTGCTTGTTTTAAAGTATTTAATTGTTGCCATaa                            <  1:2780629/99‑1 (MQ=255)                                                             cGCCTTTTTCAAACTCACTAAAACGTGGTATTTCTGGCTCAAATGTATGCGTTAAATCAACCCAAGTTGCTTGTTTTAAAGTATTTAATTGTTGCCATaa                            <  1:5187052/100‑1 (MQ=255)                                                             cGCCTTTTTCAAACTCACTAAAACGTGGTATTTCTGGATCAAATGTATGCGTTAAATCAACCCAAGTTGCTTGTTTTAAAGTATTTAATTGTTGCCATat                            <  1:933817/100‑2 (MQ=255)                                                             cGCCTTTTTCAAACTCACTAAAACGTGGTATTTCTGGATCAAATGTATGCGTTAAATCAACCCAAGTTGCTTGTTTTAAAGTATTTAATTGTTGCCATaa                            <  1:3094176/100‑1 (MQ=255)                                                                cTTTTTCAAACTCACTAAAACGTGGTATTTCTGGATCAAATGTATGCGTTAAATCAACCCAAGTTGCTTGTTTTAAAGTATTTAATTGTTGCCATAAAgg                         <  1:5550238/100‑1 (MQ=255)                                                                     tcgaaCTCACTAAAACGTGGTATTTCTAGATCAAATGTATGCGTTAAATCAACCCAAGTTGCTTGTTTTAAAGTATTTAATTGTTGCCATAAAGGATAtt                    <  1:1774274/97‑1 (MQ=255)                                                                      cAAACTCACTAAAACGTGGCATTTCTGGATCAAATGTATGCGTTAAATCAACCCAAGTTGCTTGTTTTAAAGTATTTAATTGTTGCCATAAAGGATATtg                   <  1:3282843/100‑1 (MQ=255)                                                                        aaCTCACTAAAACGTGGTATTTCTGGATCAAATGTATGCGTTAAATCAACCCAAGTTGCTTGTTTTAAAGTATTTAATTGTTGCCATAAAGGATATtgtg                 <  1:3261856/100‑1 (MQ=255)                                                                        aaCTCACTAAAACGTGGTATTTCTGGATCAAATGTATGCGTTAAATCAACCCAAGTTGCTTGTTTTAAAGTATTTAATTGTTGCCATAAAGGATATtgtg                 <  1:4426824/100‑1 (MQ=255)                                                                        aaCTCACTAAAACGTGGTATTTCTGGATCAAATGTATGCGTTAAATCAACCCAAGTTGCTTGTTTTAAAGTATTTAATTGTTGCCATAAAGGATATtgtg                 <  1:6311173/100‑1 (MQ=255)                                                                         aCTCACTAAAACGTGGTATTTCTGGATCAAATGTATGCGTTAAATCAACCCAAGTTGCTTGTTTTAAAGTATTTAATTGTTGCCATAAAGGATATtgtgg                <  1:4841818/100‑2 (MQ=255)                                                                         aCTCACTAAAACGTGGTATTTCTGGATCAAATGTATGCGTTAAATCAACCCAAGTTGCTTGTTTTAAAGTATTTAATTGTTGCCATAAAGGATATtgtgg                <  1:109057/100‑2 (MQ=255)                                                                         aCTCACTAAAACGTGGTATTTCTGGATCAAATGTATGCGTTAAATCAACCCAAGTTGCTTGTTTTAAAGTATTTAATTGTTGCCATAAAGGATATtgtgc                <  1:5719048/100‑2 (MQ=255)                                                                         aCTCACTAAAACGTGGTATTTCTGGATCAAATGTATGCGTTAAATCAACCCAAGTTGCTTGTTTTAAAGTATTTAATTGTTGCCATAAAGGATATtgtgc                <  1:4270498/100‑2 (MQ=255)                                                                          cTCACTAAAACGTGGTATTTCTGGATCAAATGTATGCGTTAAATCAACCCAAGTTGCTTGTTTTAAAGTATTTAATTGTTGCCATAAAGGATATtgtgct               <  1:295725/100‑3 (MQ=255)                                                                           tCACTAAAACGTGGTATTTCTGGATCAAATGTATGCGTTAAATCAACCCAAGTTGCTTGTTTTAAAGTATTTAATTGTTGCCATAAAGGATATTGTGTcg              <  1:6107791/100‑2 (MQ=255)                                                                            cACTAAAACGTGGTATTTCTGGATCAAATGTATGCGTTAAATCAACCCAAGTTGCTTGTTTTAAAGTATTTAATTGTTGCCATAAAGGATATTGTGTCAt             <  1:1967737/100‑1 (MQ=255)                                                                             aCTAAAACGTGGTATTTCTGGATCAAATGTATGCGTTAAATCAACCCAAGTTGCTTGTTTTAAAGTATTTAGTTGTTGCCATAAAGGATATTGTGTCATa            <  1:6097686/100‑1 (MQ=255)                                                                             aCTAAAACGTGGTATTGCTGGATCAAATGTATGCGTTAAATCAACCAAAGTTGCTTGTTTTAAAGTATTTAATTGTTGCCATAAAGGATATTGTGTCATa            <  1:5005917/100‑1 (MQ=255)                                                                                     gTGGTAGTTCTGGATCAAATGTATGCGTTAAATCAACCCATGTTGCTTGTTTTAAAGTATTTAATTGTTGCCATAAAGGATATTGTGTCATAAAATCAcc    <  1:4076923/100‑1 (MQ=255)                                                                                       ggTATTTCTGGATCAAATGTATGCGTTAAATCAACCCAAGTTGCTTGTTTTAAAGTATTTAATTGTTGCCATAAAGGATATTGTGTCATAAAATCACCCg  <  1:4112945/100‑1 (MQ=255)                                                                                               |                                                                                            CAATATTCCAACGTTGCACATAGAAACCATGATCTTTAACCGTGAATAAAGTTGAAACTTCGCCTTTTTCAAACTCACTAAAACGTGGTATTTCCGGATCAAATGTATGCGTTAAATCAACCCAAGTTGCTTGTTTTAAAGTATTTAATTGTTGCCATAAAGGATATTGTGTCATAAAATCACCCG  >  NC_007793/407851‑408036

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑