Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,119,897	C→A	M80I (ATG→ATT)	SAUSA300_RS10760 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,119,897	0	C	A	100.0%	23.5 / NA	31	M80I (ATG→ATT)	SAUSA300_RS10760	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (10/21);  total (10/21)

ATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAA  >  NC_007793/2119866‑2119993                                |                                                                                                 ttccgatctcctacgATTTCATGACTAAGTTAATTAGTCTGTCCTGTTCATCTGTGTTCTCTTCAATCCATTCATAAATAGATTGATTTAATATGTCTaa                              <  1:1842274/91‑1 (MQ=14)  agcgacctcctaccATTTCATGACTAAGTTAATTAGTCTGTCCTGTTCATCTGTGTTCTCTTCAATCCATTCATAAATAGATTGATTTAATATGTCTaac                             <  1:2003432/92‑2 (MQ=14)        ctcctaccATTTCATGACTAAGTTAATTAGTCTGTCCTGTTCATCTGTGTTCTCTTCAATCCATTCATAAATAGATTGATTTAATATGTATAATGCtgtg                       <  1:3021953/98‑1 (MQ=11)         tcctaccATTTCATGACTAAGTTAATTAGTCTGTCCTGTTCATCTGTGTTCTCTTCAATCCATTCATAAATAGATTGATTTAATATGTCTAATGCTgtgt                      <  1:715537/99‑1 (MQ=11)         cctgtccATCTCATGACTAAGTTAATTAGTCTGTCCTGTTCATCTGTGTTCTCTTCAATCCATTCATAAATAGATTGATTTAATATGTCTAATGCTgtgt                      >  1:1846213/6‑100 (MQ=2)         ccgatctaTTTCCTGACTAAGTTAATTAGTCTGTCCTGTTCATCTGTGTTCTCTTCAATCCATTCACAAATAGAGTGATTTAATATGTCTAATGCtgtca                      <  1:2263688/93‑3 (MQ=9)          cctaccATTTCATGACTAAGTTAATTAGTCTGTCCTGTTCATCTGTGTTCTCTTCAATCCCTTCCTAAATAGATTGATTTAATATGTCTAATGCTGTGta                     >  1:2872532/1‑100 (MQ=2)          cctaccATTTCATGACTAAGTTAATTAGTCTGTCCTGTTCATCTGTGTTCTCTTCAATCCATTCATAAATAGATTGATTTAATATGTCTAATGCTGTGta                     >  1:5822417/1‑100 (MQ=1)          cctaccATTTCATGACTAAGTTAATTAGTCTGTCATAATCATCTGCGTTTTCTTCAATCCATTCGTAAATAGATTGATTTAATATGTCTAATGCtgtggt                     <  1:1015559/100‑3 (MQ=2)          cctaccATTTCATGACTAAGTTAATTAGTCTGTCATAATCATCTGCGTTTTCTTCAATCCATTCGTAAATAGATTGATTTAATATGTCTAATGCtgtgca                     >  1:5617270/1‑98 (MQ=2)          cctaccATTTCATGACTAAGTTAATTAGTCTGTCATAATCATCTGCGTTTTCTTCAATCCATTCGTAAATAGATTGATTTAATATGTCTAATGCTGCGta                     >  1:1432937/1‑100 (MQ=2)          cctaacATTTCATGACTAAGTTAATTAGTCTGTCCTGTTCATCTGTGTTCTCTTCAATCCATTCATAAATAGATTGATTTAATATATCTAATGCTGTGta                     >  1:727510/1‑100 (MQ=11)          ccgcccATTTCATGACTAAGTTAATTAGTCTGTCCTGTTCATCTGTGTTCTCTTCAATCCATTCATAAACAGATTGATTTAATATGTCTAATGCTGTGta                     >  1:4059011/5‑100 (MQ=11)          ccaaccATTTCATGACTAAGTTAATTAGTCTGTCCTGTTCATCTGTGTTCTCTTTAATCCATTCATAAATAGATTGATTTAATATGTCTAATGCTGTGta                     <  1:1821872/97‑1 (MQ=0)           ctgccATTTCATGACTAAGTTAATTAGTCTGTCCTGTTCATCTGTGTTCTCTTCAATCCATTCATAAATAGATTGATTTAATATGTCTAATGCTGTGtat                    <  1:232383/97‑1 (MQ=2)           ctgccATTTCATGACTAAGTTAATTAGTCTGTCCTGTTCATCTGTGTTCTCTTCAATCCATTCATAAATAGATTGATTTAATATGTCTAATGCTGTGtat                    <  1:4646530/97‑1 (MQ=2)           ctcacATTTCATGACTAAGTTAATTAGTCTGTCCTGTTCATCTGTGTTCTCTTCAATCCATTCATAAATAGATTGATTTAATATGTCTAATGCTGTGtat                    <  1:4036766/96‑1 (MQ=2)           ctactaTTTCATGACTAAGTTAATTAGTCTGTCCTGTTCATCTGTGTTCTCTTCAATCCATTCATAAATAGATTGATTTAATATGTCTAATGCTGTGtat                    <  1:4056617/100‑1 (MQ=2)           ctaccATTTCATGACTAAGTTAATTAGTCTGTCCTGTTCATCTGTGTTCTCTTCAATCCATTCATAAATAGATTGATTTAATATGTCTAATGCTGTGtat                    <  1:2758902/100‑1 (MQ=11)           ctaccATTTCATGACTAAGTTAATTAGTCTGTCCTGTTCATCTGTGTTCTCTTCAATCCATTCATAAATAGATTGATTTAATATGTCTAATGCTGTGtat                    <  1:917267/100‑1 (MQ=11)           ctaccATTTCATGACTAAGTTAATTAGTCTGTCATAATCATCTGCGTTTTCTTCAATCCATTCGTAAATAGATTGATTTAATATGTCTAATGCTGTGtat                    <  1:4051655/100‑1 (MQ=2)           ctaccATTTCATGACTAAGTTAATTAGTCTGTCATAATCATCTGCGTTTTCTTCAATCCATTCGTAAATAGATTGATTTAATATGTCTAATGCTGTGtat                    <  1:6122751/100‑1 (MQ=2)           ctaccATTTCATGACTAAGTTAATTAGTCTGTCATAATCATCTGCGTTTTCTTCAATCCATTCGTAAATAGATTGATTTAATATGTCTAATGCTATGcat                    >  1:956122/1‑100 (MQ=2)           ctaccATATCATGACTAAGTTAATTAGTCTGTCCTGTTCATCTGTGTTCTCTTCAATCCATTCATAAATAGAGTGATTTAGTATGTCTAATGCTGTGtat                    <  1:5496132/100‑1 (MQ=2)           cctccATTTCATGACTAAGTTAATTAGTCTGTCCTGTTCATCTGTGTTCTCTTCAATCCATTCATAAATAGATTGATTTAATATGTCTAATGCTGTGtat                    >  1:3682866/4‑100 (MQ=2)           cctcattttCATGACTAAGTTAATTAGTCTGTCCTGTTCATCTGTGTTCTCTTCAATCCATTCATAAATAGATTGATTTAATATGTCTAATGCTGTGtat                    <  1:6615137/94‑1 (MQ=2)           ccaccATTTCATGACTAAGTTAATTAGTCTGTCCTGTTCATCTGTGTTCTCTTCAATCCATTCATAAATAGATTGATTTAATATGTCTAATGCTGTGtat                    <  1:1169752/98‑1 (MQ=2)           ataccATTTCATGACTAAGTTAATTAGTCTGTCCTGTTCATCTGTGTTCTCTTCAATCCATTCATAAATAGATTGATTTAATATGTCTAATGCTGTGtat                    <  1:1194277/99‑1 (MQ=11)            ttccATTTCATGACTAAGTTAATTAGTCTGTCCTGTTCATCTGTGTTCTCTTCAATCCATTCATAAATAGATTGATTTAATATGTCTAATGCTGTGtatg                   <  1:2887773/98‑2 (MQ=2)             accATTTCATGACTAAGTTAATTAGCCTGTCCTGTTCATCTGTGTTCTCTTCAATCCATTCATAAATAGATTGATTTAATATGTCTAATGCTGTGTATAg                  >  1:3074759/1‑100 (MQ=2)                             gTTAATTAGTCTGTTATAATCATCTGCGTTTTCTTCAATCCATTCGTAAATAGATTGATTTAATATGTCTAATGCTGTGTATAGATCGTTCTCATTAgtt  <  1:4942602/100‑3 (MQ=9)                                |                                                                                                 ATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAA  >  NC_007793/2119866‑2119993

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑