Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	402,447	C→T	Y229Y (TAC→TAT)	SAUSA300_RS01860 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	402,447	0	C	T	100.0%	56.1 / NA	21	Y229Y (TAC→TAT)	SAUSA300_RS01860	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/0);  total (21/0)

TATTCAATTACTACTGGAATTAATCAAAGCTTTAGTTTGCTACTTATCATTGCTATTTTCATATATAACGCCTTTAGTTACTTATTGAAGAGAAGACGTTTTTATTAAAATTAAACAAAGAGGAGCATGGATATGACAACATTGTTAAACGTAGATAGTGTGAACAAACAATAC  >  NC_007793/402367‑402540                                                                                 |                                                                                              tATTCAATTACTACTGGAATTAATCAAAGCTTTAGTTTGCTACTTATCATTGCTATTTTCATATATAACGCCTTTAGTTATTTATTGAAGAGAAGACGtt                                                                            >  1:4371385/1‑100 (MQ=255)     ctaTTACTACTGGAATTAATCAAAGCTTTAGTTTGCTACTTATCATTGCTATTTTCATATATAACGCCTTTAGTTATTTATTGAAGAGAAGACGTTTtta                                                                        >  1:699563/3‑100 (MQ=255)     cAATTACTACTGGAATTAATCAAAGCTTTAGTTTGCTACTTATCATTGCTATTTTCATATATAACGCCTTTAGTTATTTATTGAAGAGAAGACGTTTtta                                                                        >  1:5066809/1‑100 (MQ=255)      caTTACTACTGGAATTAATCAAAGCTTTAGTTTGCTACTTATCATTGCTATTTTCATATATAACGCCTTTAGTTATTTATTGAAGAGAAGACGTTTttat                                                                       >  1:379200/2‑100 (MQ=255)       aTTACTACTGGAATTAATCAAAGCTTTAGTTTGCTACTTATCATTGCTATTTTCATATATAACGCCTTTAGTTATTTATTggagagaagatcggaagagc                                                                      >  1:129487/1‑90 (MQ=255)        ttACTACTGGAATTAATCAAAGCTTTAGTTTGCTACTTATCATTGCTATTTTCATATATAACGCCTTTAGTTATTTATTGAAGAGAAGACGTTTttatta                                                                     >  1:1807111/1‑100 (MQ=255)        gtactacTGGAATTAATCAAAGCTTTAGTTTGCTACTTATCATTGCTATTTTCATATATAACGCCTTTAGTTATTTATTGAAGAGAAGACGTTTttatta                                                                     >  1:6266016/2‑100 (MQ=255)         ccctactGGAATTAATCAAAGCTTTAGTTTGCTACTTATCATTGCTATTTTCATATATAACGCCTTTAGTTATTTATTGAAGAGAAGACGTTTTTattaa                                                                    >  1:4727637/3‑100 (MQ=255)          actactGGAATTAATCAAAGCTTTAGTTTGCTACTTATCATTGCTATTTTCATATATAACGCCTTTAGTTATTTATTGAAGAGAAGACGTTTTTattaaa                                                                   >  1:5839908/1‑100 (MQ=255)          actactGGAATTAATCAAAGCTTTAGTTTGCTACTTATCATTGCTATTTTCATATATAACGCCTTTAGTTATTTACTGAAGAGAAGACGTTTTTattaaa                                                                   >  1:2972400/1‑100 (MQ=255)            tactGGAATTAATCAAAGCTTTAGTTTGCTACTTATCATTGCTATTTTCATATATAACGCCTTTAGTTATTTATTGAAGAGAAGACGTTTTTattaaaat                                                                 >  1:2882406/1‑100 (MQ=255)             actGGAATTAATCAAAGCTTTAGTTTGCTACTTATCATTGCTATTTTCATATATAACGCCTTTAGTTATTTATTGAAGAGAAGACGTTTTTattaaaatt                                                                >  1:6615916/1‑100 (MQ=255)             actGGAATTAATCAAAGCTTTAGTTTGCTACTTATCATTGCTATTTTCATATATAACGCCTTTAGTTATTTATTGAAGAGAAGACGTTTTTattaaaatt                                                                >  1:2721108/1‑100 (MQ=255)                         cAAAGCTTTAGTTTGCTACTTATCATTGCTATTTTCATATATAACGCCTTTAGTTATTTATTGAAGAGAAGACGTTTTTATTAAAATTAAACAAAgagga                                                    >  1:3725999/1‑100 (MQ=255)                                 tAGTTTGCTACTTATCATTGCTATTTTCATATATAACGCCTTTAGTTATTTATTGAAGAGAAGACGTTTCTATTAAAATTAAACAAAGAGGAGCATGGat                                            >  1:3618165/1‑100 (MQ=255)                                     ttGCTACTTATCATTGCTATTTTCATATATAACGCCTTTAGTTATTTATTGAAGAGAAGACGTTTTTATTAAAATTAAACAAAGAGGAGCATGGATATGa                                        >  1:3257965/1‑100 (MQ=255)                                          aCTTATCATTGCTATTTTCATATATAACGCCTTTAGTTATTTATTGAAGAGAAGACGTTTTTATTAAAATTAAACAAAGAGGAGCATGGATATGacaaca                                   >  1:1633886/1‑100 (MQ=255)                                                    gCTATTTTCATATATAACGCCTTTAGTTATTTATTGAAGAGAAGACGTTTTTATTAAAATTAAACAAAGAGGAGCATGGATATGACAACATTGTTAAACg                         >  1:5520792/1‑100 (MQ=255)                                                                      gCCTTTAGTTATTTATTGAAGAGAAGACGTTTTTATTAAAATTAAACAAAGAGGAGCATGGATATGACAACATTGTTAAACGTAGATAGTGTGaacaaac       >  1:1301089/1‑100 (MQ=255)                                                                      agggttAGTTATTTATTGAAGAGAAGACGATTTTATTAAAATTAAACAATGAGGAGCGTGGATATGACAACATTGTTAAACGTAGATAGCGTGaacaaac       >  1:2257005/5‑100 (MQ=255)                                                                           tAGTTATTTATTGAAGAGAAGACGTTTTTATTAAAATTAAACAAAGAGGAGCATGGATATGACAACATTGTTAAACGTAGATAGTGTGAACAAACAATAc  >  1:3112202/1‑100 (MQ=255)                                                                                 |                                                                                              TATTCAATTACTACTGGAATTAATCAAAGCTTTAGTTTGCTACTTATCATTGCTATTTTCATATATAACGCCTTTAGTTACTTATTGAAGAGAAGACGTTTTTATTAAAATTAAACAAAGAGGAGCATGGATATGACAACATTGTTAAACGTAGATAGTGTGAACAAACAATAC  >  NC_007793/402367‑402540

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑