Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,113,526	A→G	I30I (ATT→ATC)	SAUSA300_RS10715 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,113,526	0	A	G	100.0%	24.5 / NA	14	I30I (ATT→ATC)	SAUSA300_RS10715	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (2/12);  total (2/12)

CTGTATCAAATGTCTTTTTCACGCCTTGCCAACCTGGTACGATATGCCCATGAAAGTAATAAGTGCCGTTTACTACATGAATATGTGCCACTCGTTCGTTATCCTGATACAGATATCTCTTAGATCCAAAGAATTGATTTAGGTATTCTTTGCGTGCGTTATCTGTCATGATC  >  NC_007793/2113447‑2113619                                                                                |                                                                                              cTGTATCAAATGTCTTTTTCACGCCTTGCCAACCTGGTACGATATGCCCGTGAAAGTAATAAGTGTCATTTAATACATGGATATGTGCCACTcgctcgtt                                                                           <  1:1119652/100‑1 (MQ=14)  tGTATCAAATGTCTTTTTCACGCCTTGCCAACCTGGTACGATATGCCCGTGAAAGTAATAAGTGTCATTTAATACATGGATATGTGCCACTcgctcgttt                                                                          <  1:629069/100‑2 (MQ=14)   gTACCAAACGTCTTCTTCACACCTTGCCAACCTGGTACGATATGCCCATGAAAATAATAAATGTCATTCACTACATGGATATGTGCCACTAGTTCGTTAt                                                                         >  1:4960593/1‑100 (MQ=14)         aaTGTCTTTTTCACGCCTTGCCAACCTGGTACGATATGCCCGTGAAAGTAATAAGTGTCATTTAATACATGGATATGTGCCACTCGCTCGTTATTCTggt                                                                   <  1:5020927/100‑3 (MQ=255)          gtGTCTTTTTCACGCCTTGCCAACCTGGTACGATATGCCCGTGAAAGTAATAAGTGTCATTTAATACATGGATATGTGCCACTCGCTCGTTATTCTgtgt                                                                  <  1:1432218/99‑4 (MQ=255)           tGTCTTTTTCACGCTTTGCCAACCTGGTACGATATGCCCGTGAAAGTAATAAGTGTCATTTAATACATGGATATGTGCCACTCGCTCGTTATTCTGATAc                                                                 <  1:4944125/100‑1 (MQ=11)           tGTCTTTTTCACGCCTTGCCAACCTGGTACGATATGCCCGTGAAAGTAATAAGTGTCATTTAATACATGGATATGTGCCACTCGCTCGTTATTCTGATAc                                                                 <  1:3350828/100‑1 (MQ=255)           tGTCTTTTTCACGCCTTGCCAACCTGGTACGATATGCCCGTGAAAGTAATAAGTGTCATTTAATACATGGATATGTGCCACTCGCTCGTTATTCTGATAc                                                                 <  1:4708655/100‑1 (MQ=255)                      gatcTTGCCGACCTGGTACGATATGCCCATGAAAATAATAAATGTCATTCACTACATGGATATGTGCCACTCGTTCGTTATCCCGATACAGATATCTCtt                                                      <  1:1616764/97‑1 (MQ=14)                      caccTTGCCAACCTGGTACGATATGCCCATGAAAATAATAAATGTCATTCACTACATGGATATGTGCCACTCGTTCGTTATCCCGATACAGATATCTCtt                                                      <  1:1184040/98‑1 (MQ=14)                      caccTTGCCAACCTGGTACGATATGCCCATGAAAATAATAAATGTCATTCACTACATGGATATGTGCCACTCGTTCGTTATCCCGATACAGATATCTCtt                                                      <  1:4285366/98‑1 (MQ=14)                      caccTTGCCAACCTGGTACGATATGCCCATGAAAATAATAAATGTCATTCACTACATGGATATGTGCCACCCGTTCGTTATCCCGATACAGATATCTCtt                                                      <  1:4040322/98‑1 (MQ=14)                             ccAACCTGGTACGATATGCCCATGAAAATAATAAATGTCATTCACTACATGGATATGTGCCACTCGTTCGTTATCCCGATACAGATATCCCCTAGATcgg                                               >  1:2053131/1‑98 (MQ=14)                                                                          tacATGGATATGTGCCACTCGTTCGTTATCCCGATACAGATATCTCTTAGATCCGAAAAATTGATTTAAGTATTCTTTGCGTGCGTTATCTGTCAtagtc  <  1:6184296/100‑5 (MQ=14)                                                                                |                                                                                              CTGTATCAAATGTCTTTTTCACGCCTTGCCAACCTGGTACGATATGCCCATGAAAGTAATAAGTGCCGTTTACTACATGAATATGTGCCACTCGTTCGTTATCCTGATACAGATATCTCTTAGATCCAAAGAATTGATTTAGGTATTCTTTGCGTGCGTTATCTGTCATGATC  >  NC_007793/2113447‑2113619

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑