Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,113,512	C→T	G35D (GGC→GAC)	SAUSA300_RS10715 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,113,512	0	C	T	100.0%	24.7 / NA	14	G35D (GGC→GAC)	SAUSA300_RS10715	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (2/12);  total (2/12)

TTGCTTTATATATGTTTCAAGCTCTTCAGCTGTATCAAATGTCTTTTTCACGCCTTGCCAACCTGGTACGATATGCCCATGAAAGTAATAAGTGCCGTTTACTACATGAATATGTGCCACTCGTTCGTTATCCTGATACAGATATCTCTTAGATCCA  >  NC_007793/2113418‑2113574                                                                                               |                                                               ttGCTTTATATATTTTTCAAGCTCTTCCGCTGTATCAAATGTCGTTTTCACGCCTTGCCAACCTGGTACGATATGCCCGTGAAAGTAATAAGTGTCAttt                                                           <  1:4294568/100‑1 (MQ=255)                              cTGTATCAAATGTCTTTTTCACGCCTTGCCAACCTGGTACGATATGCCCGTGAAAGTAATAAGTGTCATTTAATACATGGATATGTGCCACTcgctcgtt                              <  1:1119652/100‑1 (MQ=14)                               tGTATCAAATGTCTTTTTCACGCCTTGCCAACCTGGTACGATATGCCCGTGAAAGTAATAAGTGTCATTTAATACATGGATATGTGCCACTcgctcgttt                             <  1:629069/100‑2 (MQ=14)                                gTACCAAACGTCTTCTTCACACCTTGCCAACCTGGTACGATATGCCCATGAAAATAATAAATGTCATTCACTACATGGATATGTGCCACTAGTTCGTTAt                            >  1:4960593/1‑100 (MQ=14)                                      aaTGTCTTTTTCACGCCTTGCCAACCTGGTACGATATGCCCGTGAAAGTAATAAGTGTCATTTAATACATGGATATGTGCCACTCGCTCGTTATTCTggt                      <  1:5020927/100‑3 (MQ=255)                                       gtGTCTTTTTCACGCCTTGCCAACCTGGTACGATATGCCCGTGAAAGTAATAAGTGTCATTTAATACATGGATATGTGCCACTCGCTCGTTATTCTgtgt                     <  1:1432218/99‑4 (MQ=255)                                        tGTCTTTTTCACGCTTTGCCAACCTGGTACGATATGCCCGTGAAAGTAATAAGTGTCATTTAATACATGGATATGTGCCACTCGCTCGTTATTCTGATAc                    <  1:4944125/100‑1 (MQ=11)                                        tGTCTTTTTCACGCCTTGCCAACCTGGTACGATATGCCCGTGAAAGTAATAAGTGTCATTTAATACATGGATATGTGCCACTCGCTCGTTATTCTGATAc                    <  1:3350828/100‑1 (MQ=255)                                        tGTCTTTTTCACGCCTTGCCAACCTGGTACGATATGCCCGTGAAAGTAATAAGTGTCATTTAATACATGGATATGTGCCACTCGCTCGTTATTCTGATAc                    <  1:4708655/100‑1 (MQ=255)                                                   gatcTTGCCGACCTGGTACGATATGCCCATGAAAATAATAAATGTCATTCACTACATGGATATGTGCCACTCGTTCGTTATCCCGATACAGATATCTCtt         <  1:1616764/97‑1 (MQ=14)                                                   caccTTGCCAACCTGGTACGATATGCCCATGAAAATAATAAATGTCATTCACTACATGGATATGTGCCACTCGTTCGTTATCCCGATACAGATATCTCtt         <  1:1184040/98‑1 (MQ=14)                                                   caccTTGCCAACCTGGTACGATATGCCCATGAAAATAATAAATGTCATTCACTACATGGATATGTGCCACTCGTTCGTTATCCCGATACAGATATCTCtt         <  1:4285366/98‑1 (MQ=14)                                                   caccTTGCCAACCTGGTACGATATGCCCATGAAAATAATAAATGTCATTCACTACATGGATATGTGCCACCCGTTCGTTATCCCGATACAGATATCTCtt         <  1:4040322/98‑1 (MQ=14)                                                          ccAACCTGGTACGATATGCCCATGAAAATAATAAATGTCATTCACTACATGGATATGTGCCACTCGTTCGTTATCCCGATACAGATATCCCCTAGATcgg  >  1:2053131/1‑98 (MQ=14)                                                                                               |                                                               TTGCTTTATATATGTTTCAAGCTCTTCAGCTGTATCAAATGTCTTTTTCACGCCTTGCCAACCTGGTACGATATGCCCATGAAAGTAATAAGTGCCGTTTACTACATGAATATGTGCCACTCGTTCGTTATCCTGATACAGATATCTCTTAGATCCA  >  NC_007793/2113418‑2113574

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑