Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,113,415	C→A	Q67H (CAG→CAT)	SAUSA300_RS10715 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,113,415	0	C	A	90.9%	13.1 / NA	11	Q67H (CAG→CAT)	SAUSA300_RS10715	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (0/10);  minor base G (0/1);  total (0/11)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCTCCTCTAAAATAAAGTTAGTTGCTTCTGTTCCTCATATTCCAAACCCTGTTGCTTTATATATGTTTCAAGCTCTTCAGCTGTATCAAATGTCTTTTTCACGCCTTGCCAACCTGGTACGATATGCCCATG  >  NC_007793/2113367‑2113498                                                 |                                                                                    gatctcttAAAATAAAGTTAGTTGCTTCTGTCCTTCGTATTCCAAACCATGTTGCTTTATATATGTTTCAAGCTCTTCAGCTGTATCAAACGTCttcttc                                  <  1:4018640/93‑1 (MQ=11)      tttAAAATAAAGTTAGTTGCTTCTGTCCTTCGTATTCCAAACCATGTTGCTTTATATATGTTTCAAGCTCTTCAGCTGTATCAAACGTCTTCTTCACAcc                             <  1:177493/98‑1 (MQ=11)      catgaaaTAAAGTTAGTTGCTTCTGTTCCTCATATTCCAAACCATGTTGCTTTATATATTTTTCAAGCTCTTCCGCTATATCAAATGTCTTTTTCACGcc                             <  1:382851/96‑1 (MQ=14)       ttAAAAGAAAGTTAGTTGCTTCTGTTCCTCATATTCCAAACCATGTTGCTTTATATATTTTTCAAGCTCTTCCGCTGTATCAAATGTCTTTTTCACGCCt                            <  1:3378141/99‑1 (MQ=14)         atgaTAAGGTTAGTTGCTACTGTCCTTCGTGTTCCAAACCGTGTTGCTTTATATATGTTTCAAGCTCTTCAGCTGTATCAAACGTCGTCTTCACACCTTg                          <  1:5837845/97‑1 (MQ=255)           aaTAAAGTTAGTTGCTTATGTTACTCATATTCCAAACCATGTTGCTTTATATATTTTTCAAGCTCTTCCGCTGTATCAAATGTCTTTTTCACGCCTTGcc                        <  1:4568279/100‑1 (MQ=14)            aTAAAGTTAGTTGCTTCTGTTCCTCATATTCCAAACCATGTTGCTTTATATATTTTTCAAGCTCTTCCGCTGTATCAAATGTCTTTTTCACGCCTTGCCa                       <  1:2141278/100‑1 (MQ=255)                  ctcttccgatCTGTTCCTCATATTCCAAACCATGTTGCTTTATATATTTTTCAAGCTCTTCCGCTGTATCAAATGTCTTTTTCACGCCTTGCCAACCTgg                 <  1:6324825/91‑1 (MQ=14)                   tcttccgatCTGTTCCCCATATTCCAAACCATGTTGCTTTATATATTTTTCAAGCTCTTCCGCTGTATCAAATGTCTTTTTCACGCCTTGCCAACCTGGt                <  1:6229980/92‑1 (MQ=14)                   tagttGCTTCTGTCCTTCGTATTCCAAACCATGTTGCTTTATATATGTTTCAAGCTCTTCAGCTGTATCAAACGTCTTCTTCACACCTTGCCAACCTGGt                <  1:6559205/100‑1 (MQ=14)                                 ccTCATATTCCAAACCATGTTGCTTTATATATTTTTCAAGCTCTTCCGCTGTATCAAATGTCTTTTTCACGCCTTGCCAACCTGGTACGATATGCCCGTg  <  1:2290817/100‑1 (MQ=17)                                                 |                                                                                    CCTCCTCTAAAATAAAGTTAGTTGCTTCTGTTCCTCATATTCCAAACCCTGTTGCTTTATATATGTTTCAAGCTCTTCAGCTGTATCAAATGTCTTTTTCACGCCTTGCCAACCTGGTACGATATGCCCATG  >  NC_007793/2113367‑2113498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑