Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,113,334	T→G	I11L (ATA→CTA)	SAUSA300_RS10710 ←	acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,113,334	0	T	G	100.0%	38.3 / NA	16	I11L (ATA→CTA)	SAUSA300_RS10710	acetyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (2/14);  total (2/14)

CTTAACTTAGCTATATCCCCAATAAGCTCGTCACGTTGCTTCTTGTACTCATCACGTTGTTTTCTCATCTTCTTCAACCTAGCTTCCATTACGCCTATTTGGAATCCTGTTTCATAGTTCACTTTC  >  NC_007793/2113237‑2113362                                                                                                  |                             ctTAACTTACCTATATCCCCAATAAGCTCATCTCGTTGCCTCTTGTACTCATCACGTTGTTTTCTCATCTTCTTCAACCTAGCGTCCATTACACCTAgtt                            <  1:1847414/100‑1 (MQ=255)      cTTACCTATATCCCCAATAAGCTCATCTCGTTGCTTCTTGTACTCATCACGTTGTTTTCTCATCTTCTTCAACCTAGCGTCCATTACACCTAGTTGGaaa                       <  1:5213454/100‑2 (MQ=255)           cTATATCCCCAATAAGCTCATCTCGTTGCTTCTTGTACTCATCACGTTGTTTTCTCATCTTCTTCAACCTAGCGTCCATTACACCTAGTTGGAACCCTgg                  <  1:3041088/100‑2 (MQ=255)           cTATATCCCCAATAAGCTCATCTCGTTGCTTCTTGTACTCATCACGTTGTTTTCTCATCTTCTTCAACCTAGCGTCCATTACACCTAGTTGGAACCCTgc                  <  1:516544/100‑2 (MQ=255)           cTATATCCCCAATAAGCTCATCTCGTTGCTTCTTGTACTCATCACGTTGTTTTCTCATCTTCTTCAACCTAGCGTCCATTACACCTAGTTGGAACCCTga                  <  1:4125618/100‑2 (MQ=255)            tgtatCCCCAATAAGCTCATCTCGTTGCTTCTTGTACTCATCACGTTGTTTTCTCATCTTCTTCAACCTAGCGTCCATTACACCTAGTTGGAACCCTGtt                 <  1:1901454/98‑1 (MQ=255)                tCCCCATTAAGCTCATCTCGTTGCTTCTTGTACTCATCACGTTGTTTTCTCATCTTCTTCAACCTAGCGTCCATTACACCTAGTTGGAACCCTGTTTCAt             <  1:714117/100‑1 (MQ=255)                tCCCCAATAAGCTCATCTCGTTGCTTCTTGTACTCATCACGTTGTTTTCTCATCTTCTTCAACCTAGCGTCCATTACACCTAGTTGGAACCCTGTTTCAt             <  1:2679683/100‑1 (MQ=255)                tCCCCAATAAGCTCATCTCGTTGCTTCTTGTACTCATCACGTTGTTTTCTCATCTTCTTCAACCTAGCGTCCATTACACCTAGTTGGAACCCTGTTTCAt             <  1:2123368/100‑1 (MQ=255)                 ccccCATAAGCTCATCTCGTTGCTTCTTGTACTCATCACGTTGTTTTCTCATCTTCTTCAACCTAGCGTCCATTACACCTAGTTGAAACCCTGTTTCATa            >  1:4977265/1‑100 (MQ=255)                  cccAATAAGCTCATCTCGTTGCTTCTTGTACTCATCACGTTGTTTTCTCATCTTCTTCAACCTAGCGTCCATTACACCTAGTTGGAACCCTGTTTCATAg           <  1:2010765/100‑1 (MQ=255)                   ccAATAAGCTCATCTCGTTGCTTCTTGTACTCATCACGTTGTTTTCTCATCTTCTTCAACCTAGCGTCCATTACACCTAGTTGGAACCCTGTTTCATAGt          >  1:5072436/1‑100 (MQ=255)                    cAATAAGCTCATCTCGTTGCTTCTTGTACTCATCACGTTGTTTTCTCATCTTCTTCAACCTAGCGTCCATTACACCTAGTTGGAACCCTGTTTCATAGtt         <  1:6355599/100‑1 (MQ=255)                      aTAAGCTCATCTCGTTGCTTCTTGTACTCATCACGTTGTTTTCTCATCTTCTTCAACCTAGCGTCCATTACGCCTAGTTGGAGCCCTGTTTCATAGTTCa       <  1:3590348/100‑1 (MQ=255)                      aTAAGCTCATCTCGTTGCTTCTTGTACTCATCACGTTGTTTTCTCATCTTCTTCAACCTAGCGTCCATTACACCTAGTTGGAACCCTGTTTCATAGTTCa       <  1:77492/100‑1 (MQ=255)                        aaGCTCATCTCGTTGCTTCTTGTACTCATCACGTTGTTTTCTCATCTTCTTCAACCTAGCGTCCATTACACCTAGTTGGAACCCTGTTTCATAGTTCatg     <  1:1277790/100‑3 (MQ=255)                           cTCATCTCGTTGCTTCTTGTACTCATCACGTTGTTTTCTCATCTTCTTCAACCTAGCGTCCATTACGCCTAGTTGGAACCCTGTTTCATAGTTCattctg  <  1:6447816/100‑6 (MQ=255)                                                                                                  |                             CTTAACTTAGCTATATCCCCAATAAGCTCGTCACGTTGCTTCTTGTACTCATCACGTTGTTTTCTCATCTTCTTCAACCTAGCTTCCATTACGCCTATTTGGAATCCTGTTTCATAGTTCACTTTC  >  NC_007793/2113237‑2113362

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑