Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	402,167	G→A	S136N (AGT→AAT)	SAUSA300_RS01860 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	402,167	0	G	A	100.0%	100.7 / NA	39	S136N (AGT→AAT)	SAUSA300_RS01860	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (39/0);  total (39/0)

CTGCAGACAGTTATTGCTTTTTTAGTATTGCTTATTTTTGTGGTAGGGCACGCTGCAGCAAATGCAATACTATTCTTTTTAATACCATTTTTTGCTAGTGCTATTTTCAATACACAATTTACACTGTTTAATAGAAGGTTTGATGACAGAATACCAAAAATTGCAGATAAGAATTACACTGAAAAGCGATTG  >  NC_007793/402070‑402261                                                                                                  |                                                                                               cTGCAGACAGTTATTGCTTTTTTAGTATTGCTTATTTTTGTGGTAGGGCACGCTGCAGCAAATGCAATACTATTCTTTTTAATACCATTTTTTGCTAATg                                                                                              >  1:1041880/1‑100 (MQ=255)  tGCAGACAGTTATTGCTTTTTTAGTATTGCTTATTTTTGTGGTAGGGCACGCTGCAGCAAATGCAATACTATTCTTTTTAATACCATTTTTTGCTAATGc                                                                                             >  1:883999/1‑100 (MQ=255)   gCAGACAGTTATTGCTTTTTTAGTATTGCTTATTTTTGTGGTAGGGCACGCTGCAGCAAATGCAATACTATTCTTTTTAATACCATTTTTTGCTAATGCt                                                                                            >  1:5363655/1‑100 (MQ=255)   gCAGACAGTTATTGCTTTTTTAGTATTGCTTATTTTTGTGGTAGGGCACGCTGCAGCAAATGCAATACTATTCTTTTTAATACCATTTTTTGCTAATGCt                                                                                            >  1:4725308/1‑100 (MQ=255)   gCAGACAGTTATTGCTTTTTTAGTATTGCTTATTTTTGTGGTAGGGCACGCTGCAGCAAATGCAATACTATTCTTTTTAATACCATTTTTTGCTAATGCt                                                                                            >  1:2910278/1‑100 (MQ=255)   gCAGACAGTTATTGCTTTTTTAGTATTGCTTATTTTTGTGGTAGGGCACGCTGCAGCAAATGCAATACTATTCTTTTTAATACCATTTTTTGCTAATGCt                                                                                            >  1:3968534/1‑100 (MQ=255)   gCAGACAGTTATTGCTTTCTTAGTATTGCTTATTTTTGTGGTAGGGCACGCTGCAGCAAATGCAATACTATTCTTTTTAATACCATTTTTTGCTAATGCt                                                                                            >  1:1245245/1‑100 (MQ=255)   acAGACAGTTATTGCTTTTTTAGTATTGCTTATTTTTGTGGTAGGGCACGCTGCAGCAAATGCAATACTATTCTTTTTAATACCATTTTTTGCTAATGCt                                                                                            >  1:1159758/2‑100 (MQ=255)    taGACAGTTATTGCTTTTTTAGTATTGCTTATTTTTGTGGTAGGGCACGCTGCAGCAAATGCAATACTATTCTTTTTAATACCATTTTTTGCTAATGCTa                                                                                           >  1:6062794/2‑100 (MQ=255)    taGACAGTTATTGCTTTTTTAGTATTGCTTATTTTTGTGGTAGGGCACGCTGCAGCAAATGCAATACTATTCTTTTTAATACCATTTTTTGCTAATGCTa                                                                                           >  1:3611820/2‑100 (MQ=255)    cAGACAGTTATTGCTTTTTTAGTATTGCTTATTTTTGTGGTAGGGCACGCTGCAGCAAATGCAATACTATTCTTTTTAATACCATTTTTTGCTAATGCTa                                                                                           >  1:5650847/1‑100 (MQ=255)    cAGACAGTTATTGCTTTTTTAGTATTGCTTATTTTTGTGGTAGGGCACGCTGCAGCAAATGCAATACTATTCTTTTTAATACCATTTTTTGCTAATGCTa                                                                                           >  1:280988/1‑100 (MQ=255)       ccAGTTATTGCTTTTTTAGTATTGCTTATTTTTGTGGTAGGGCACGCTGCAGCAAATGCAATACTAGTCTTTTTAATACCATTTTTTGCTAATGCTAttt                                                                                        >  1:4356509/2‑100 (MQ=255)           gcaTTGCTTTTTTAGTATTGCTTATTTTTGTGGTAGGGCACGCTGCAGCAAATGCAATACTATTCTTTTTAATACCATTTTTTGCTAATGCTATTTTCaa                                                                                    >  1:475427/3‑100 (MQ=255)            tATTGCTTTTTTAGTATTGCTTATTTTTGTGGTAGGGCACGCTGCAGCAAATGCAATACTATTCTTTTTAATACCATTTTTTGCTAATGCTATTTTCAAt                                                                                   >  1:967072/1‑100 (MQ=255)                          caTTGCTTATTTTTGTGGTAGGGCACGCTGCAGCAAATGCAATACTATTCTTTTTAATACCATTTTTTGCTAATGCTATTTTCAATACACAATTTACACt                                                                     >  1:320820/2‑100 (MQ=255)                           aTTGCTTATTTTTGTGGTAGGGCACGCTGCAGCAAATGCAATACTATTCTTTTTAATACCATTTTTTGCTAATGCTATTTTCAATACACAATTTACACTg                                                                    >  1:5324247/1‑100 (MQ=255)                             tGCTTATTTTTGTGGTAGGGCACGCTGCAGCAAATGCAATACTATTCTTTTTAATACCATTTTTTGCTAATGCTATTTTCAATACACAATTTACACTGtt                                                                  >  1:5080065/1‑100 (MQ=255)                                 tATTTTTGTGGTAGGGCACGCTGCAGCAAATGCAATACTATTCTTTTTAATACCATTTTTTGCTAATGCTATTTTCAATACACAATTTACACTGTTTAAt                                                              >  1:2398008/1‑100 (MQ=255)                                      ctgtgGTAGGGCACGCTGCAGCAAATGCAATACTATTCTTTTTAATACCATTTTTTGCTAATGCTATTTTCAATACACAATTTACACTGTTTAATAGAAg                                                         >  1:2685220/2‑100 (MQ=255)                                        gtgGTAGGGCACGCTGCAGCAAATGCAATACTATTCTTTTTAATACCATTTTTTGCTAATGCTATTTTCAATACACAATTTACACTGTTTAATAGAAGGt                                                       >  1:1034585/1‑100 (MQ=255)                                        gtgGTAGGGCACGCTGCAGCAAATGCAATACTATTCTTTTTAATACCATTTTTTGCTAATGCTATTTTCAATACACAATTTACACTGTTTAATAGAAGGt                                                       >  1:5744313/1‑100 (MQ=255)                                        gtgGTAGGGCACGCTGCAGCAAATGCAATACTATTCTTTTTAATACCATTTTTTGCTAATGCTATTTTCAATACACAATTTACACTGTTTAATAGAAGGt                                                       >  1:5611305/1‑100 (MQ=255)                                        gtgGTAGGGCACGCTGCAGCAAATGCAATACTATTCTTTTTAATACCATTTTTTGCTAATGCTATTTTCAATACACAATTTACACTGTTTAATAGAAGGt                                                       >  1:362075/1‑100 (MQ=255)                                        ctgGTAGGGCACGCTGCAGCAAATGCAATACTATTCTTTTTAATACCATTTTTTGCTAATGCTATTTTCAATACACAATTTACACTGTTTAATAGAAGGt                                                       >  1:3679519/2‑100 (MQ=255)                                          cttAGGGCACGCTGCAGCAAATGCAATACTATTCTTTTTAATACCATTTTTTGCTAATGCTATTTTCAATACACAATTTACACTGTTTAATAGAAGGttt                                                     >  1:2209096/3‑100 (MQ=255)                                             aGGGCACGCTGCAGCAAATGCAATACTATTC‑TTTTAATACCATTTTTTGCTAATGCTATTTTCAATACACAATTTACACTGTTTAATAGAAGGTTtgatg                                                 >  1:4727660/1‑100 (MQ=255)                                             aGGGCACGCTGCAGCAAATGCAATACTATTC‑TTTTAATACCATTTTTTGCTAATGCTATTTTCAATACACAATTTACACTGTTTAATAGAAGGTTtgatg                                                 >  1:3881858/1‑100 (MQ=255)                                               ggCACGCTGCAGCAAATGCAATACTATTCTTTTTAATACCATTTTTTGCTAATGCTATTTTCAATACACAATTTACACTGTTTAATAGAAGGTTtgatga                                                >  1:1494790/1‑100 (MQ=255)                                               agCACGCTGCAGCAAATGCAATACTATTCTTTTTAATACCATTTTTTGCTAATGCTATTTTCAATACACAATTTACACTGTTTAATAGAAGGTTtgatga                                                >  1:5481866/2‑100 (MQ=255)                                                 cACGCTGCAGCAAATGCAATACTATTCTTTTTAATACCATTTTTTGCTAATGCTATTTTCAATACACAATTTACACTGTTTAATAGAAGGTTTGATGACa                                              >  1:2988838/1‑100 (MQ=255)                                                 agcGCTGCAGCAAATGCAATACTATTCTTTTTAATACCATTTTTTGCTAATGCTATTTTCAATACACAATTTACACTGTTTAATAGAAGGTTTGA‑GACAg                                             >  1:4586631/3‑100 (MQ=255)                                                  aCGCTGCAGCAAATGCAATACTATTCTTTTTAATACCATTTTTTGCTAATGCTATTTTCAATACACAATTTACACTGTTTAATAGAAGGTTTGATGACAg                                             >  1:3017743/1‑100 (MQ=255)                                                  aCGCTGCAGCAAATGCAATACTATTCTTTTTAATACCATTTTTTGCTAATGCTATTTTCAATACACAATTTACACTGTTTAATAGAAGGTTTGATGACAg                                             >  1:3437697/1‑100 (MQ=255)                                                    acTGCAGCAAATGCAATACTATTCTTTTTAATACCATTTTTTGCTAATGCTATTTTCAATACACAATTTACACTGTTTAATAGAAGGTTTGATGACAGaa                                           >  1:2567663/2‑100 (MQ=255)                                                             aaTGCAATACTATTCTTTTTAATACCATTTTTTGCTAATGCTATTTTCAATACACAATTTACACTGTTTAATAGTAGGTTTGATGCCAGAATACCaagaa                                  >  1:4837991/1‑100 (MQ=255)                                                                  aaTACTATTCTTTTTAATACCATTTTTTGCTAATGCTATTTTCAATACACAATTTACACTGTTTAATAGAAGGTTTGATGACAGAATACCAAAAATTGCa                             >  1:2496047/1‑100 (MQ=255)                                                                        aTTCTTTTTAATACCATTTTTTGCTAATGCTATTTTCAATACACAATTTACACTGTTTAATAGAAGGTTTGATGACAGAATACCAAAAATTGCAGATAAg                       >  1:6486015/1‑100 (MQ=255)                                                                                             tGCTAATGCTATTTTCAATACACAATTTACACTGTTTAATAGAAGGTTTGATGACAGAATACCAAAAATTGCAGATAAGAATTACACTGAAAAGCGATTg  >  1:364341/1‑100 (MQ=255)                                                                                                  |                                                                                               CTGCAGACAGTTATTGCTTTTTTAGTATTGCTTATTTTTGTGGTAGGGCACGCTGCAGCAAATGCAATACTATTCTTTTTAATACCATTTTTTGCTAGTGCTATTTTCAATACACAATTTACACTGTTTAATAGAAGGTTTGATGACAGAATACCAAAAATTGCAGATAAGAATTACACTGAAAAGCGATTG  >  NC_007793/402070‑402261

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑