Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,113,246	G→C	A40G (GCT→GGT)	SAUSA300_RS10710 ←	acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,113,246	0	G	C	100.0%	46.3 / NA	18	A40G (GCT→GGT)	SAUSA300_RS10710	acetyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (1/17);  total (1/17)

CCAGCTCTTCGTTACGCTCTCTTAACTTAGCTATATCCCCAATAAGCTCGTCACGTTGCTTCTTGTACTCATCACGTTGTTTTCTCATCTTCTTCAACCTAGCTTCCATTACGCCTATTTGGAAT  >  NC_007793/2113217‑2113341                              |                                                                                                ctcgCTCTTTGTTGCGTTCTCTTAACTTACCTATATCCCCAATAAGCTCATCTCGTTGCTTCTTGTACTCATCACGTTGTTTTCTCATCTTCTTCAACCt                           <  1:3020436/97‑1 (MQ=255) ccAGCTCTTTGTTGCGTTCTCTTAACTTACCTATATCCCCAATAAGCTCATCTCGTTGCTTCTTGTACTCATCACGTTGTTTTCTCATCTTCTTCAACCt                           <  1:2713815/100‑1 (MQ=255) ccAGCTCTTTGTTGCGTTCTCTTAACTTACCTATATCCCCAATAAGCTCATCTCGTTGCTTCTTGTACTCATCACGTTGTTTTCTCATCTTCTTCAACCt                           >  1:4482760/1‑100 (MQ=255) ccAGCTCTTTGTTGCGTTCTCTTAACTTACCTATATCCCCAATAAGCTCATCTCGTTGCTTCTTGTACTCATCACGTTGTTTTCTCATCTTCTTCAACCt                           <  1:4000838/100‑1 (MQ=255) ccAGCTCTTTGTTGCGTTCTCTTAACTTACCTATATCCCCAATAAGCTCATCTCGTTGCTTCTGGTACTCATCACGTTGTTTTCTCATCTTCTTCAACCt                           <  1:688685/100‑1 (MQ=255) ccAGCACTTTGTTGCGTTCTCTTAACTTACCTATATCCCCAATAAGCTCATCTCGTTGCTTCTTGTACTCATCACGTTGTTTTCTCATCTTCTTCAACCt                           <  1:5690221/100‑1 (MQ=255)   aGCTCTTTGTTGCGTTCTCTTAACTTACCTATATCCCCAATAAGCTCATCTCGTTGCTTCTTGTACTCATCACGTTGTTTTCTCATCTTCTTCAACCTAg                         <  1:1566697/100‑1 (MQ=255)    gCTCTTTGTTGCGTTCTCTTAACTTACCTATATCCCCAATAAGCTCATCTCGTTGCTTCTTGTACTCATCACGTTGTTTTCTCATCTTCTTCAACCTAgt                        <  1:900139/100‑2 (MQ=255)     ctctTTGTTGCGTTCTCTTAACTTACCTATATCCCCAATAAGCTCATCTCGTTGCTTCTTGTACTCATCACGTTGTTTTCTCATCTTCTTCAACCTAgag                       <  1:4369498/100‑3 (MQ=255)     ctctTTGTTGCGTTCTCTTAACTTACCTATATCCCCAATAAGCTCATCTCGTTGCTTCTTGTACTCATCACGTTGTTTTCTCATCTTCTTCAACCTAGcg                       <  1:5327704/100‑2 (MQ=255)     ctctTTGTTGCGTTCTCTTAACTTACCTATATCCCCAATAAGCTCATCTCGTTGCTTCTTGTACTCATCACGTTGTTTTCTCATCTTCTTCAACCTAGcg                       <  1:2272290/100‑2 (MQ=255)          tgTTGCGTTCTCTTAACTTACCTATATCCCCAATAAGCTCATCTCGTTGCTTCTTGTACTCATCACGTTGTTTTCTCATCTTCTTCAACCTAGCGTCCAt                  <  1:5273051/99‑1 (MQ=255)             tgcgttctctTAACTTACCTATATCCCCAATAAGCTCATCTCGTTGCTTCTTGTACTCATCACGTTGTTTTCTCATCTTCTTCAACCTAGCGTCCATTAc               <  1:1546978/95‑1 (MQ=255)               cgttctctTAACTTACCTATATCCCCAATAAGCTCATCTCGTTGCTTCTTGTACTCATCACGTTGTTTTCTCATCTTCTTCAACCTAGCGTCCATTAcac             <  1:1541887/97‑3 (MQ=255)                gttctctTAACTTACCTATATCCCCAATAAGCTCATCTCGTTGCTTCTTGTACTCATCACGTTGTTTTCTCATCTTCTTCAACCTAGCTTCCATTACAcc            <  1:6299136/98‑1 (MQ=255)                gttctctTAACTTACCTATATCCCCAATAAGCTCATCTCGTTGCTTCTTGTACTCATCACGTTGTTTTCTCATCTTCTTCAACCTAGCGTCCATTACAcc            <  1:2781762/98‑1 (MQ=255)                     ctTAACTTACCTATATCCCCAATAAGCTCATCTCGTTGCCTCTTGTACTCATCACGTTGTTTTCTCATCTTCTTCAACCTAGCGTCCATTACACCTAgtt       <  1:1847414/100‑1 (MQ=255)                          cTTACCTATATCCCCAATAAGCTCATCTCGTTGCTTCTTGTACTCATCACGTTGTTTTCTCATCTTCTTCAACCTAGCGTCCATTACACCTAGTTGGaaa  <  1:5213454/100‑2 (MQ=255)                              |                                                                                                CCAGCTCTTCGTTACGCTCTCTTAACTTAGCTATATCCCCAATAAGCTCGTCACGTTGCTTCTTGTACTCATCACGTTGTTTTCTCATCTTCTTCAACCTAGCTTCCATTACGCCTATTTGGAAT  >  NC_007793/2113217‑2113341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑