Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,113,230	A→G	R45R (CGT→CGC)	SAUSA300_RS10710 ←	acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,113,230	0	A	G	100.0%	26.3 / NA	12	R45R (CGT→CGC)	SAUSA300_RS10710	acetyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (1/11);  total (1/11)

CCAGCTCTTCGTTACGCTCTCTTAACTTAGCTATATCCCCAATAAGCTCGTCACGTTGCTTCTTGTACTCATCACGTTGTTTTCTCATCTTCTTCAACCTAGCTTCCAT  >  NC_007793/2113217‑2113325              |                                                                                                ctcgCTCTTTGTTGCGTTCTCTTAACTTACCTATATCCCCAATAAGCTCATCTCGTTGCTTCTTGTACTCATCACGTTGTTTTCTCATCTTCTTCAACCt           <  1:3020436/97‑1 (MQ=255) ccAGCTCTTTGTTGCGTTCTCTTAACTTACCTATATCCCCAATAAGCTCATCTCGTTGCTTCTTGTACTCATCACGTTGTTTTCTCATCTTCTTCAACCt           <  1:2713815/100‑1 (MQ=255) ccAGCTCTTTGTTGCGTTCTCTTAACTTACCTATATCCCCAATAAGCTCATCTCGTTGCTTCTTGTACTCATCACGTTGTTTTCTCATCTTCTTCAACCt           <  1:4000838/100‑1 (MQ=255) ccAGCTCTTTGTTGCGTTCTCTTAACTTACCTATATCCCCAATAAGCTCATCTCGTTGCTTCTTGTACTCATCACGTTGTTTTCTCATCTTCTTCAACCt           >  1:4482760/1‑100 (MQ=255) ccAGCTCTTTGTTGCGTTCTCTTAACTTACCTATATCCCCAATAAGCTCATCTCGTTGCTTCTGGTACTCATCACGTTGTTTTCTCATCTTCTTCAACCt           <  1:688685/100‑1 (MQ=255) ccAGCACTTTGTTGCGTTCTCTTAACTTACCTATATCCCCAATAAGCTCATCTCGTTGCTTCTTGTACTCATCACGTTGTTTTCTCATCTTCTTCAACCt           <  1:5690221/100‑1 (MQ=255)   aGCTCTTTGTTGCGTTCTCTTAACTTACCTATATCCCCAATAAGCTCATCTCGTTGCTTCTTGTACTCATCACGTTGTTTTCTCATCTTCTTCAACCTAg         <  1:1566697/100‑1 (MQ=255)    gCTCTTTGTTGCGTTCTCTTAACTTACCTATATCCCCAATAAGCTCATCTCGTTGCTTCTTGTACTCATCACGTTGTTTTCTCATCTTCTTCAACCTAgt        <  1:900139/100‑2 (MQ=255)     ctctTTGTTGCGTTCTCTTAACTTACCTATATCCCCAATAAGCTCATCTCGTTGCTTCTTGTACTCATCACGTTGTTTTCTCATCTTCTTCAACCTAgag       <  1:4369498/100‑3 (MQ=255)     ctctTTGTTGCGTTCTCTTAACTTACCTATATCCCCAATAAGCTCATCTCGTTGCTTCTTGTACTCATCACGTTGTTTTCTCATCTTCTTCAACCTAGcg       <  1:2272290/100‑2 (MQ=255)     ctctTTGTTGCGTTCTCTTAACTTACCTATATCCCCAATAAGCTCATCTCGTTGCTTCTTGTACTCATCACGTTGTTTTCTCATCTTCTTCAACCTAGcg       <  1:5327704/100‑2 (MQ=255)          tgTTGCGTTCTCTTAACTTACCTATATCCCCAATAAGCTCATCTCGTTGCTTCTTGTACTCATCACGTTGTTTTCTCATCTTCTTCAACCTAGCGTCCAt  <  1:5273051/99‑1 (MQ=255)              |                                                                                                CCAGCTCTTCGTTACGCTCTCTTAACTTAGCTATATCCCCAATAAGCTCGTCACGTTGCTTCTTGTACTCATCACGTTGTTTTCTCATCTTCTTCAACCTAGCTTCCAT  >  NC_007793/2113217‑2113325

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑