Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,111,571	C→T	V53I (GTT→ATT)	SAUSA300_RS10685 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,111,571	0	C	T	100.0%	18.1 / NA	11	V53I (GTT→ATT)	SAUSA300_RS10685	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (2/9);  total (2/9)

TTTTAATCATTTCCCACACTCCCTTATATTTTCAAACAACTGACCCACTTTAATAACTGCATCCCTTTTAACTTGTTTCTCGTACTTCTCTTTCGCTTCTTCTTTACTCTCTGCCTCAACAACTGTAAACCTTTGATTACTCTTAGCTTT  >  NC_007793/2111515‑2111664                                                         |                                                                                              ccaccgtCATTTCCCACACTCCCTTATATTTTCAAACAACTGACTCACTTTAATAATTGCATCCCTTTTAACTTGCGCCTCGCACTTCTCTTTCGcttct                                                    <  1:2438005/94‑1 (MQ=14)   tccgatctttCCCCCACTCCCTTATATTTTCAAACAACTGACTCACTTTAATAATTGCATCCCTTTTAACTTGCGCCTCGTACTTCTCTTTCGcttctgg                                                  <  1:4781932/93‑3 (MQ=17)              ccACGCTTCCTTATATTTTCAAATAACTGACTCACTTTAATAATTGCATCCCTTTTAACTTGTTTCTCGTACTTCTCTTTCGCTTCTTCTTTACTCTCTg                                       <  1:2989507/100‑1 (MQ=18)                    tCCCTTATATTTTCAAACAACTGACTCACTTTAATAATTGCATCCCTTTTAACTTGCGCCTCGTACTTCTCTTTCGCTTCTTCTTTACTCTCTGCCTcat                                 <  1:3031839/100‑2 (MQ=17)                     cccTAATATTTTCAAACAACTGACTCACTTTAATAATTGCATCACTTTTAACTTGCGCCTCGTACTTCTCTTTCGCTTCTTCTTTACTCTCTGccagatc                                >  1:678923/1‑95 (MQ=17)                      ccTTGTATTTTCAAACAACTGACTCACTTTAATAATTGCATCCCTTTTAACTTGCGCCTCGTACTTCTCTTTCGCTTCTTCTTTACTCTCTGCCTcaaca                               <  1:1744293/100‑1 (MQ=17)                           tatTTTCAAACAACTGACTCACTTTAATAATTGCATCCCTTTTAACTTGCGCCTCGTACTTCTCTTTCGCTTCTTCTTTACTCTCTGCCTCAACAACTGt                          <  1:3316700/100‑1 (MQ=17)                             atttCAAATAACTGACTCACTTTAATAATTGCATCCCTTTTAACTTGTTTCTCGTACTTCTCTTTCGCTTCTTCTTTACTCTCTGCCTCAACAACTGTaa                        >  1:6466512/2‑100 (MQ=255)                                    acaacTGACTCACTTTAATAATTGCATCCCTTTTAACTTGCGCCTCGTACTTCTCTTTCGCTTCTTCTTTACTCTCTGCCTCAACAACTGTAAAcgtctg                 <  1:366099/100‑6 (MQ=17)                                      aacTGACTCACTTTAATAATTGCATCCCTTTTAACTTGTTTCTCGTACTTCTCTTTCGCTTCTTCTTTACTCTCTGCCTCAACAACTGTAAACCTTTGAt               <  1:2596221/100‑1 (MQ=255)                                                   taataaTTGCATCCCTTTTAACTTGTTTCTCGTACTTCTCTTTCGCTTCTTCTTTACTCTCTGCCTCAACAACTGTAAACCTTTGATTGCTATTAGCttt  <  1:6059695/100‑1 (MQ=255)                                                         |                                                                                              TTTTAATCATTTCCCACACTCCCTTATATTTTCAAACAACTGACCCACTTTAATAACTGCATCCCTTTTAACTTGTTTCTCGTACTTCTCTTTCGCTTCTTCTTTACTCTCTGCCTCAACAACTGTAAACCTTTGATTACTCTTAGCTTT  >  NC_007793/2111515‑2111664

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑