Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,111,103	C→T	A13A (GCG→GCA)	SAUSA300_RS10675 ←	transcriptional activator RinB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,111,103	0	C	T	100.0%	17.7 / NA	13	A13A (GCG→GCA)	SAUSA300_RS10675	transcriptional activator RinB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (2/11);  total (2/11)

CCGCTCGAAAGACGTAATCACTCGGCGCCTCTACATCATCATTAGCCGTCATCATAATATATACTTGCTCAGTTACATACTTACCTAACTCATACATCGCTAGTAAGAATAATAATCTTAGTATTTGCTTAATCATTTTTTATCTAC  >  NC_007793/2111006‑2111152                                                                                                  |                                                  ccGCTCGAAAGACGTAATCACTCGGTGCCTCTACATCATCATTAGCCGTCATCATAATATATACTTGCTCCGTTACATACTTACCTAGCTCATACATTGc                                                 >  1:1712763/1‑100 (MQ=14) ccGCTCGAAAGACGTAATCACTCGGCGCCTCTACATCATCATTAGCCGTCATCATAATATATACTTGCTCCGTTACATACTTACCTAGCTCATACATTGc                                                 <  1:3028779/100‑1 (MQ=255)  cgcgcgAAAGACGTAATCACTCGGCGCCTCTACATCATCATTAGCCGTCATCATAATATATACTTGCTCCGTTACATACTTACCTAGCTCATACATTGCt                                                <  1:277965/100‑1 (MQ=14)      cGAAAGACGTAATCACTCGGCGCCTCTACATCATCATTAGCCGTCATCATAATATATACTTGCTCCGTTACATACTTACCTAGCTCATACATTGCTAgca                                            <  1:265603/100‑3 (MQ=11)      cGAAAGACGTAATCACTCGGCGCCTCTACATCATCATTAGCCGTCATCATAATATATACTTGCTCCGTTACATACTTACCTAGCTCATACATTGCTAgca                                            <  1:6193939/100‑3 (MQ=11)         aaGACGTAATCACTCGGCGCCTCTACATCATCATTAGCCGTCATCATAATATATACTTGCTCCGTTACATACTTACCTAGCTCATACATTGCTAGCAAgc                                         <  1:5740780/100‑2 (MQ=11)         aaGACGTAATCACTCGGCGCCTCTACATCATCATTAGCCGTCATCATAATATATACTTGCTCCGTTACATACTTACCTAGCTCATACATTGCTAGCAAGa                                         <  1:5322803/100‑1 (MQ=11)                     cttccgatctctACATCATCATTAGCCGTCATCATAATATATACTTGCTCAGTTACATACATGCCTAACTCGTACATTGCTAGTAAGAATAATAATCttg                             <  1:3830587/92‑2 (MQ=11)                                          tctgCCGTCATCATAATATATACTTGCTCAGCTACATACTTACCTAACTCGTACATTGCTAGTAAGAATAATAATCTTAGTATTTGCTTAATCAtttccc        <  1:3958920/97‑4 (MQ=11)                                            ggCCGTCATCATAATATATACTTGCGCAGTTACATACTTACCTAACTCGTACATTGCTAGTAAGAATAATAATCTTAGTATTTGCTTAATCAtttcccac      >  1:5830470/2‑95 (MQ=11)                                              ccGTCATCATAATATATACTTGCTCAGTTACATACTTACCTAACTCGTACATTGCTAGTAAGAATAATAATCTTAGTATTTGCTTAATCAtttcccagtg    <  1:4074214/100‑8 (MQ=11)                                              ccGTCATCATAATATATACTTGCTCAGTTACATACTTACCTAACTCGTACATTGCTAGTAAGAATAATAATCTTAGTATTTGCTTAATCAtttcccacaa    <  1:5787890/100‑8 (MQ=11)                                                gTCATCATAATATATACTTGCTCAGTTACATACTTACCTAACTCGTACATTGCTAGTAAGAATAATAATCTTAGTATTTGCTTAATCAtttcccacactt  <  1:1704189/100‑10 (MQ=11)                                                                                                  |                                                  CCGCTCGAAAGACGTAATCACTCGGCGCCTCTACATCATCATTAGCCGTCATCATAATATATACTTGCTCAGTTACATACTTACCTAACTCATACATCGCTAGTAAGAATAATAATCTTAGTATTTGCTTAATCATTTTTTATCTAC  >  NC_007793/2111006‑2111152

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑