Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	402,035	A→G	N92S (AAT→AGT)	SAUSA300_RS01860 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	402,035	0	A	G	100.0%	66.7 / NA	27	N92S (AAT→AGT)	SAUSA300_RS01860	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/1);  total (26/1)

GAATCAAAGACGTGCATTGAAGTATAAGCAACTTGTAGATAAAGAGGTAGATATCGATGCAACAGATCAATATGAGTTGCTTGCGAATCGTTATGTTTTAAATGGAAGTATATTAAGTATTCTGCAGACAGTTATTGCTTTTTTAGTATTGCTTATTTTTGTGGTAGGGCACGCTGCAGCAA  >  NC_007793/401949‑402130                                                                                       |                                                                                                gaATCAAAGACGTGCATTGAAGTATAAGCAACTTGTAGATAAAGAGGTAGATATCGATGCAACAGATCAATATGAGTTGCTTGCGAGTCGTTATGTTTTa                                                                                    >  1:4693254/1‑100 (MQ=255)      aaaGACGTGCATTGAAGTATAAGCAACTTGTAGATAAAGAGGTAGATATCGATGCAACAGATCAATATGAGTTGCTTGCGAGTCGTTATGTTTTAAATgg                                                                               >  1:2221527/1‑100 (MQ=255)         gACGTGCATTGAAGTATAAGCAACTTGTAGATAAAGAGGTAGATATCGATGCAACAGATCAATATGAGTTGCTTGCGAGTCGTTACGtttaagatcggaa                                                                            >  1:5328830/1‑90 (MQ=255)           cGTGCATTGAAGTATAAGCAACTTGTAGATAAAGAGGTAGATATCGATGCAACAGATCAATATGAGTTGCTTGCGAGTCGTTATGTTTTAAATGGAAGta                                                                          >  1:2183750/1‑100 (MQ=255)                  tGAAGTATAAGCAACTTGTAGATAAAGAGGTAGATATCGATGCAACAGATCAATATGAGTTGCTTGCGAGTCGTTATGTTTTAAATGGAAGTATATTAAg                                                                   >  1:1573996/1‑100 (MQ=255)                            gCAACTTGTAGATAAAGAGGTAGATATCGATGCAACAGATCAATATGAGTTGCTTGCGAGTCGTTATGTTTTAAATGGAAGTATATTAAGTATTCTGCAg                                                         >  1:6057120/1‑100 (MQ=255)                               aCTTGTAGATAAAGAGGTAGATATCGATGCAACAGATCAATATGAGTTGCTTGCGAGTCGTTATGTTTTAAATGGAAGTATATTAAGTATTCTGCAGACa                                                      >  1:3939702/1‑100 (MQ=255)                                   gTAGATAAAGAGGTAGATATCGATGCAACAGATCAATATGAGTTGCTTGCGAGTCGTTATGTTTTAAATGGAAGTATATTAAGTATTCTGCAGACAGTTa                                                  >  1:2912061/1‑100 (MQ=255)                                        tAAAGAGGTAGATATCGATGCAACAGATCAATATGCGTTGCTTGCGAGTCGTTATGTTTTAAATGGCAGTATATTAAGTATTCTGCAGACCGTTATTGCt                                             >  1:1284336/1‑100 (MQ=255)                                                              aCAGATCAATATGAGTTGCTTGCGAGTCGTTATGTTTTAAATGGAAGTATATTAAGTATTCTGCAGACAGTTATTGCTTTTTTAGTATTGCTTATTTTtg                       >  1:1464982/1‑100 (MQ=255)                                                                      atatGAGTTGCTTGCGAGTCGTTATGTTTTAAATGGAAGTATATTAAGTATTCTGCAGACAGTTATTGCTTTTTTAGTATTGCTTATTTTTGTGGTAggg               >  1:5614222/1‑100 (MQ=255)                                                                          gAGTTGCTTGCGAGTCGTTATGTTTTAAATGGAAGTATATTAAGTATTCTGCAGACAGTTATTGCTTTTTTAGTATTGCTTATTTTTGTGGTAGGGCAca           >  1:1912064/1‑99 (MQ=255)                                                                          gAGTTGCTTGCGAGTCGTTATGTTTTAAATGGAAGTATATTAAGTATTCTGCAGACAGTTATTGCTTTTTTAGTATTGCTTATTTTTGTGGTAGGGCACg           >  1:382870/1‑100 (MQ=255)                                                                              tgcttgcGAGTCGTTATGTTTTAAATGGAAGTATATTAAGTATTCTGCAGACAGTTATTGCTTTTTTAGTATTGCTTATTTTTGTGGTAGGGCagatcgg       >  1:3480065/1‑94 (MQ=255)                                                                              tgcttgcGAGTCGTTATGTTTTAAATGGAAGTATATTAAGTATTCTGCAGACAGTTATTGCTTTTTTAGTATTGCTTATTTTTGTGGTAGGGCACGCTgc       >  1:4006568/1‑100 (MQ=255)                                                                              tgcttgcGAGTCGTTATGTTTTAAATGGAAGTATATTAAGTATTCTGCAGACAGTTATTGCTTTTTTAGTATTGCTTATTTTTGTGGTAGGGCACGCTgc       >  1:4475800/1‑100 (MQ=255)                                                                              tgcttgcGAGTCGTTATGTTTTAAATGGAAGTATATTAAGTATTCTGCAGACAGTTATTGCTTTTTTAGTATTGCTTATTTTTGTGGTAGGGCACGCTgc       >  1:662007/1‑100 (MQ=255)                                                                              tgcttgcGAGTCGTTATGTTTTAAATGGAAGTATATTAAGTATTCTGCAGACAGTTATTGCTTTTTTAGTATTGCTTATTTTTGTGGCCGGGCagatcgg       >  1:3293767/1‑94 (MQ=255)                                                                              tgcttgcGAGTCGTTATGTTTTAAATGGAAGTATATTAAGTATTCTGCAGACAGTTATTGCTTTTTTAGTATTGCTTATTTTTGTGGCCGGGCagatcgg       >  1:6501634/1‑94 (MQ=255)                                                                              tgcttgcGAGTCGTTATGTTTTAAATGGAAGTATATTAAGTATTCTGCAGACAGTTATTGCTTTTTTAGTATTGCTTATTTTTGTGGCAgatcggaagag       >  1:2008713/1‑90 (MQ=255)                                                                              tgcttgcGAGTCGTTATGTTTTAAATGGAAGTATATTAAGTATTATGCAGACAGTTATTGCTTTTTTAGTATTGCTTATTTTCGTGGCAgatcggaagag       >  1:3568408/1‑90 (MQ=255)                                                                               gcttgcGAGTCGTTATGTTTTAAATGGAAGTATATTAAGTATTCTGCAGACAGTTATTGCTTTTTTAGTATTGCTTATTTTTGTGGTAGGGCACgtaaga      >  1:2908105/1‑95 (MQ=255)                                                                               gcttgcGAGTCGTTATGTTTTAAATGGAAGTATATTAAGTATTCTGCAGACAGTTATTGCTTTTTTAGTATTGCTTATTTTTGTGGTAGGGCACGCTgca      >  1:12214/1‑100 (MQ=255)                                                                               gcttgcGAGTCGTTATGTTTTAAATGGAAGTATATTAAGTATTCTGCAGACAGTTATTGCTTTTTTAGTATTGCTTATTTTTGTGATAGGGCAcaagatc      >  1:4925962/1‑94 (MQ=255)                                                                                  ttccgaTCTTTATGTTTTAAATGGAAGTATATTAAGTATTCTGCAGACAGTTATTGCTTTTTTAGTATTGCTTATTTTTGTGGTAGGGCACGCTgcagca   <  1:5160261/95‑1 (MQ=255)                                                                                  tgcGAGTCGTTATGTTTTAAATGGAAGTATATTAAGTATTCTGCAGACAGTTATTGCTTTTTTAGTATTGCTTATTTTTGTGGTAGGGCACGCTgcagca   <  1:2812916/100‑1 (MQ=255)                                                                                  tgcGAGTCGTTATGTTTTAAATGGAAGTATATTAAGTATTCTGCAGACAGTTATTGCTTTTTTAGTATTGCTTATTTTTGTGGTAGGGCACGCTgcagca   >  1:603684/1‑100 (MQ=255)                                                                                   gcGAGTCGTTATGTTTTAAATGGAAGTATATTAAGTATTCTGCAGACAGTTATTGCTTTTTTAGTATTGCTTATTTTTGTGGTAGGGCACGCTGCAGCaa  >  1:4273987/1‑100 (MQ=255)                                                                                       |                                                                                                GAATCAAAGACGTGCATTGAAGTATAAGCAACTTGTAGATAAAGAGGTAGATATCGATGCAACAGATCAATATGAGTTGCTTGCGAATCGTTATGTTTTAAATGGAAGTATATTAAGTATTCTGCAGACAGTTATTGCTTTTTTAGTATTGCTTATTTTTGTGGTAGGGCACGCTGCAGCAA  >  NC_007793/401949‑402130

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑