Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,099,226	A→T	G694G (GGT→GGA)	SAUSA300_RS10590 ←	phage tail tape measure protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,099,226	0	A	T	100.0%	12.2 / NA	7	G694G (GGT→GGA)	SAUSA300_RS10590	phage tail tape measure protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (2/5);  total (2/5)

CTCGCCAATCTCCAGTAAATAAACTTGAGAAGAACTTAATTAAACCTAGTATGATATTTAAAGTTCCTTGTATCACACCTTTTATATTCTCCCAAGTACTGACAATCAAGGCTTTAACCGCCGGCCAAATAAATTGCATCA  >  NC_007793/2099150‑2099290                                                                             |                                                                 ctcGCCAATTACCAGTGAATAAACTTGAAAAGAACTTAATAAAGCCAAGTATTATATTTAAAGCACCTTGTATTACTCCTTTTATATTCTCCCAAGTACt                                           >  1:5863472/1‑100 (MQ=255)  tcGCCAGTTACCAGTGAATAAACTTGAAAAGAACTTAATAAAGCCAAGTATTATATTTAAAGCACCTTGTATTACTCCTTTTATATTCTCCCAAGTACTg                                          <  1:2735956/100‑1 (MQ=255)  tcGCCAATTACCAGTGAATAAACTTGAAAAGAACTTAATAAAGCCAAGTATTATATTTAAAGCACCTTGTATTACTCCTTTTATATTCTCCCAAGTACTg                                          <  1:6060518/100‑1 (MQ=255)    gCCAATTACCAGTGAATAAACTTGAGAAGAACTTAATAAAGCCAAGTATTATATTTAAAGCACCTTGTATTACTCCTTTTATATTCTCCCAAGTACTGAc                                        <  1:6431194/100‑1 (MQ=255)        attaccAGTGAATAAACTTGAAAAGAACTTAATAAAGCCAAGTATTATATTTAAAGCACCTTGTATTACTCCTTTTATATTCTCCCAAGTACTGACAATc                                    <  1:1275287/96‑1 (MQ=255)         gtaccAGTGAATAAACTTGAAAAGAACTTAATAAAGCCAAGTATTATATTTAAAGCACCTTGTATTACTCCTTTTATATTCTCCCAAGTACTGACAATCa                                   >  1:3349795/4‑100 (MQ=255)                                          aagccaaGTATTATATTTAAAGCACCTTGTATTACTCCTTTTATATTCTCCCAAGTACTGACAATCAAGGCTTTAACCGCCGGCCAAATAAATTGCATCa  <  1:6531184/94‑1 (MQ=255)                                                                             |                                                                 CTCGCCAATCTCCAGTAAATAAACTTGAGAAGAACTTAATTAAACCTAGTATGATATTTAAAGTTCCTTGTATCACACCTTTTATATTCTCCCAAGTACTGACAATCAAGGCTTTAACCGCCGGCCAAATAAATTGCATCA  >  NC_007793/2099150‑2099290

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑