Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,099,190	T→A	L706F (TTA→TTT)	SAUSA300_RS10590 ←	phage tail tape measure protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,099,190	0	T	A	100.0%	10.4 / NA	6	L706F (TTA→TTT)	SAUSA300_RS10590	phage tail tape measure protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (2/4);  total (2/4)

CTCGCCAATCTCCAGTAAATAAACTTGAGAAGAACTTAATTAAACCTAGTATGATATTTAAAGTTCCTTGTATCACACCTTTTATATTCTCCCAAGTACTGACAATCA  >  NC_007793/2099150‑2099257                                         |                                                                    ctcGCCAATTACCAGTGAATAAACTTGAAAAGAACTTAATAAAGCCAAGTATTATATTTAAAGCACCTTGTATTACTCCTTTTATATTCTCCCAAGTACt          >  1:5863472/1‑100 (MQ=255)  tcGCCAGTTACCAGTGAATAAACTTGAAAAGAACTTAATAAAGCCAAGTATTATATTTAAAGCACCTTGTATTACTCCTTTTATATTCTCCCAAGTACTg         <  1:2735956/100‑1 (MQ=255)  tcGCCAATTACCAGTGAATAAACTTGAAAAGAACTTAATAAAGCCAAGTATTATATTTAAAGCACCTTGTATTACTCCTTTTATATTCTCCCAAGTACTg         <  1:6060518/100‑1 (MQ=255)    gCCAATTACCAGTGAATAAACTTGAGAAGAACTTAATAAAGCCAAGTATTATATTTAAAGCACCTTGTATTACTCCTTTTATATTCTCCCAAGTACTGAc       <  1:6431194/100‑1 (MQ=255)        attaccAGTGAATAAACTTGAAAAGAACTTAATAAAGCCAAGTATTATATTTAAAGCACCTTGTATTACTCCTTTTATATTCTCCCAAGTACTGACAATc   <  1:1275287/96‑1 (MQ=255)         gtaccAGTGAATAAACTTGAAAAGAACTTAATAAAGCCAAGTATTATATTTAAAGCACCTTGTATTACTCCTTTTATATTCTCCCAAGTACTGACAATCa  >  1:3349795/4‑100 (MQ=255)                                         |                                                                    CTCGCCAATCTCCAGTAAATAAACTTGAGAAGAACTTAATTAAACCTAGTATGATATTTAAAGTTCCTTGTATCACACCTTTTATATTCTCCCAAGTACTGACAATCA  >  NC_007793/2099150‑2099257

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑