Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	381,188	A→G	S327G (AGC→GGC)	SAUSA300_RS01745 →	lipoate‑‑protein ligase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	381,188	0	A	G	100.0%	169.4 / NA	61	S327G (AGC→GGC)	SAUSA300_RS01745	lipoate‑‑protein ligase A
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (61/0);  total (61/0)

AAGATGTGGAAGAAGCATTACAAGGAACAAAAATGACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTAGCAATGTTACGGTAGAAGCATTAGTGGATATGATTTTAAGTTAATATTGTTATTTTATGTATGCTGAATCATTGGAAGTGTTTGCTTGCTCTTGAA  >  NC_007793/381099‑381284                                                                                          |                                                                                                 aaGATGTGGCAGAAGCATTACAAGGAACAAAAATGACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTAc                                                                                        >  1:3715278/1‑100 (MQ=255) aaGATGTGGAAGAAGCATTACAAGGAACAAAAATGACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTAc                                                                                        >  1:2571689/1‑100 (MQ=255)  cgATGTGGAAGAAGCATTACAAGGAACAAAAATGACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACg                                                                                       >  1:6470910/2‑100 (MQ=255)      gtgGAAGAAGCATTACAAGGAACAAAAATGACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAAtagatcggaag                                                                                   >  1:1797278/1‑90 (MQ=255)      gtgGAAGAAGCATTACAAGGAACAAAAATGACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAg                                                                                   >  1:11308/1‑100 (MQ=255)           tcacaccttACAAGGAACAAAAATGACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCa                                                                              >  1:1114538/8‑100 (MQ=255)              agCATTACAAGGAACAAAAATGACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGagatcgga                                                                           >  1:1929769/1‑93 (MQ=255)                acttACAAGGAACAAAAATGACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGt                                                                         >  1:5492254/3‑100 (MQ=255)                 gttACAAGGAACAAAAATGACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTg                                                                        >  1:700641/2‑100 (MQ=255)                 cttACAAGGAACAAAAATGACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAgatcggaaa                                                                        >  1:2988841/2‑92 (MQ=255)                  ttACAAGGAACAAAAATGACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTgg                                                                       >  1:3089099/1‑100 (MQ=255)                  ttACAAGGAACAAAAATGACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTgg                                                                       >  1:2858470/1‑100 (MQ=255)                   tACAATGAACAAAAATGACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGa                                                                      >  1:6123087/1‑100 (MQ=255)                   tACAAGGAACAAAAATGACAAGAGAAGATTTAACGCATCAGTTAAAGCACTTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGa                                                                      >  1:5030800/1‑100 (MQ=255)                   tACAAGGAACAAAAATGACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGTATTAGTGGa                                                                      >  1:192698/1‑100 (MQ=255)                   tACAAGGAACAAAAATGACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGa                                                                      >  1:5713926/1‑100 (MQ=255)                   tACAAGGAACAAAAATGACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGa                                                                      >  1:3802918/1‑100 (MQ=255)                   tACAAGGAACAAAAATGACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGa                                                                      >  1:2869076/1‑100 (MQ=255)                   tACAAGGAACAAAAATGACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGa                                                                      >  1:3927249/1‑100 (MQ=255)                   tACAAGGAACAAAAATGACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGa                                                                      >  1:1168349/1‑100 (MQ=255)                   tACAAGGAACAAAAATGACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGa                                                                      >  1:6627639/1‑100 (MQ=255)                   tACAAGGAACAAAAATGACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGa                                                                      >  1:5607033/1‑100 (MQ=255)                   tACAAGGAACAAAAATGACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGa                                                                      >  1:1296370/1‑100 (MQ=255)                   tACAAGGAACAAAAATGACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGAGGa                                                                      >  1:1878470/1‑100 (MQ=255)                   tACAAGGAACAAAAATGACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGCAGAAGCATTAGTGGa                                                                      >  1:5506315/1‑100 (MQ=255)                   tACAAGGAAAAAAAATGACAAGAGAAGATTTAAAGCATCAGTTAAAGCAATTAGAAATCGTTTATTATTTTGACAATGTTACGGTAGAAGCATTAGAGGa                                                                      >  1:3803988/1‑100 (MQ=255)                       aGGAACAAAAATGACAAGAGGAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGATATg                                                                  >  1:2251242/1‑100 (MQ=255)                          caCAAAAATGACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGATATGAtt                                                               >  1:453925/2‑100 (MQ=255)                              caaaTGACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGATATGATTTTaa                                                           >  1:2853668/2‑100 (MQ=255)                              aaaaTGACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGATATGATTTTaa                                                           >  1:3135623/1‑100 (MQ=255)                                cttGACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGATATGATTTTAAGt                                                         >  1:1868692/3‑100 (MQ=255)                                agtGACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGATATGATTTTAAGt                                                         >  1:396147/3‑100 (MQ=255)                                aaTGACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGATATGATTTTAAGt                                                         >  1:1302532/1‑100 (MQ=255)                                aaTGACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGATATGATTTTAAGt                                                         >  1:3932383/1‑100 (MQ=255)                                 ttGACAAGAGAAGATTTAACGCATCAGATAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAAAAGCATTAGTGGATATGATTTTAAGtt                                                        >  1:4206774/2‑100 (MQ=255)                                 accccAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGATATGATTTTAAGtt                                                        >  1:3630115/5‑100 (MQ=255)                                 aTGACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGATATGATTTTAAGtt                                                        >  1:853071/1‑100 (MQ=255)                                 aTGACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGATATGATTTTAAGtt                                                        >  1:684599/1‑100 (MQ=255)                                  tGACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGATATGATTTTAAGTTa                                                       >  1:470587/1‑100 (MQ=255)                                  tGACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGATATGATTTTAAGTTa                                                       >  1:2164396/1‑100 (MQ=255)                                  tGACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGATATGATTTTAAGTTa                                                       >  1:1437630/1‑100 (MQ=255)                                  tGACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGATATGATTTTAAGTTa                                                       >  1:3875798/1‑100 (MQ=255)                                   tccAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGATCTGATTTTAAGTTaa                                                      >  1:5993713/3‑100 (MQ=255)                                   gACAAGAGAAGATTTAACGCATCAGTTAATGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGATATGATTTTAAGTTaa                                                      >  1:1022752/1‑100 (MQ=255)                                   gACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGATATGATTTTAAGTTaa                                                      >  1:4125256/1‑100 (MQ=255)                                   gACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGATATGATTTTAAGTTaa                                                      >  1:5425222/1‑100 (MQ=255)                                   gACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGATATGATTTTAAGTTaa                                                      >  1:894317/1‑100 (MQ=255)                                   gACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGATATGATTTTAAGTTaa                                                      >  1:500303/1‑100 (MQ=255)                                   gACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGATATGATTTTAAGTTaa                                                      >  1:432328/1‑100 (MQ=255)                                                 tAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGATATGATTTTAAGTTAATATTGTTATTTtat                                        >  1:5753107/1‑100 (MQ=255)                                                 ccaCGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGATATGATTTTAAGTTAATATTGTTATTTtat                                        >  1:1639882/3‑100 (MQ=255)                                                  ctcGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGATATGATTTTAAGTTAATATTGTTATTTtatg                                       >  1:1540535/3‑100 (MQ=255)                                                  aaCGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGATATGATTTTAAGTTAATATTGTTATTTtatg                                       >  1:3231007/1‑100 (MQ=255)                                                      cctCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGATATGATTTTAAGTTAATATTGTTATTTtatgtatg                                   >  1:5202588/3‑100 (MQ=255)                                                       ctCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGATATGATTTTAAGTTAATATTGTTATTTTATGTACGc                                  >  1:5032792/2‑100 (MQ=255)                                                         cAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGATATGATTTTAAGTTAATATTGTTATTTTATGTATGCTg                                >  1:3637760/1‑100 (MQ=255)                                                         cAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGATATGATTTTAAGTTAATATTGTTATTTTATGTATGCTg                                >  1:3918499/1‑100 (MQ=255)                                                              aaaGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGATATGATTTTAAGTTAATATTGTTATTTTATGTACGCTGaagat                           >  1:5191917/1‑97 (MQ=255)                                                                       ggACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGATATGATTTTAAGTTAATATTGTTATTTTATGTATGCTGAATCATTGGAAgtg                  >  1:2569738/2‑100 (MQ=255)                                                                       aGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGATATGATTTTAAGTTAATATTGTTATTTTATGTATGCTGAATCATTGGACgtg                  >  1:27693/1‑100 (MQ=255)                                                                                       tttGGCAATGCTACGGTAGAAGCATTAGTGGATATGATTTTAAGTTAATATTGTTATTTTATGTATGCTGAATCATTGGAAGTGTTTGCTTGCTCTTGaa  >  1:4715106/1‑100 (MQ=255)                                                                                          |                                                                                                 AAGATGTGGAAGAAGCATTACAAGGAACAAAAATGACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTAGCAATGTTACGGTAGAAGCATTAGTGGATATGATTTTAAGTTAATATTGTTATTTTATGTATGCTGAATCATTGGAAGTGTTTGCTTGCTCTTGAA  >  NC_007793/381099‑381284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑