| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_007793 | 380,670 | C→T | S154L (TCG→TTG) | SAUSA300_RS01745 → | lipoate‑‑protein ligase A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_007793 | 380,670 | 0 | C | T | 86.7% | 18.0 / ‑3.8 | 15 | S154L (TCG→TTG) | SAUSA300_RS01745 | lipoate‑‑protein ligase A |
| Reads supporting (aligned to +/- strand): ref base C (1/1); new base T (13/0); total (14/1) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.33e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.60e-01 | |||||||||||
ATGTGGTACAAAGCGGTAGAAATGATTTAACATTGAATGGTAAAAAAGTGTCAGGCGCCGCAATGACATTAATGAATAATCGTATTTATGGCGGTTATTCGCTATTACTTGATGTTAATTATGAAGCAATGGATAAAG‑TGTTAAAGCCTAA‑TCGCAAAAAGA > NC_007793/380571‑380732 | aTGTGGTACAAAGCGGTAGAAATGATTTAACATTGAATGGTAAAAAAGTGTCAGGCGCCGCAATGACATTAATGAATAATCGTATTTATGGCGGGTTTTc > 1:340136/1‑100 (MQ=255) ggTACAAAGCGGTAGAAATGATTTAACATTGAATGGTAAAAAAGTGTCAGGCGCCGCAATGACATTAATGAATAATCGTATTTATGGCGGTTTTTTGcct > 1:4373784/1‑98 (MQ=255) ggTACAAAGCGGTAGAAATGATTTAACATTGAATGGTAAAAAAGTGTCAGGCGCCGCAATGACATTAATGAATAATCGTATTTATGGCGGGTTTTCCCtt > 1:5467258/1‑99 (MQ=255) ggTACAAAGCGGTAGAAATGATTTAACATTGAATGGTAAAAAAGTGTCAGGCGCCGCAATGACATTAATGAATAATCGTATTTATGGCGGGTATTTGCTa > 1:1117297/1‑100 (MQ=255) caaGCGGTAGAAATGATTTAACATTGAATGGTAAAAAAGTGTCAGGCGCCGCAATGACATTAATGAATAATCGTATTTATGGCGGTTATTTGCTCTTTCt > 1:4858656/2‑100 (MQ=255) aaaGCGGTAGAAATGATTTAACATTGAATGGTAAAAAAGTGTCAGGCGCCGCAATGACATTAATGAATAATCGTATTTATGGCGGTTATTTGccctttat > 1:5506707/1‑93 (MQ=255) aggCGGTAGAAATGATTTAACATTGAATGGTAAAAAAGTGTCAGGCGCCGCAATGACATTAATGAATAATCGTATTTATGGCGGCTATTTGCTATTTCtt > 1:1035833/3‑100 (MQ=255) tAGAAATGATTTAACATTGAATGGTAAAAAAGTGTCAGGCGCCGCAATGACATTAATGAATAATCGTATTTATGGCGGCTTTTTTCCATTACTTTTTGtt > 1:5174638/1‑100 (MQ=255) aGAAATGATTTAACATTGAATGGTAAAAAAGTGTCAGGCGCCGCAATGACATTAATGAATAATCGTATTTATGGCGGTTATTTGCTTTTACTTGTTGTTa > 1:3168238/1‑100 (MQ=255) cATTGAATGGTAAAAAAGTGTCAGGCGCCGCAATGACATTAATGAATAATCGTATTTATGGCGGTTATTCGCTATTACTTGATGTTAATTATGAAGCAAt < 1:54689/100‑1 (MQ=255) ctGGTAAAAAAGTGTCAGGCGCCGCAATGACATTAATGAATAATCGTATTTATGGCGGTTTTTTCCTTTTATTTGGTGGTTTTTTTGAAAAAAAGGATaa > 1:2628528/2‑100 (MQ=255) cctggCGCCGCAACGACATTAATGAATAATCGTATTTATGGCGGTTATTTTCTCTTTATTTATGTTTATTATTAAGAAAAAGGTAAAG‑AGTTTAAgacca > 1:4432411/4‑96 (MQ=255) aGGCGCCGCAATGACATTAATGAATAATCGTATTTATGGCGGTTTTTTCGTTTTATTTTTTTTTAATTTTTAAGCCAAGGGTAAAG‑AGTTtaatacacaa > 1:3972312/1‑93 (MQ=255) ggCGCCGCAATGACATTAATGAATAATCGTATTTATGGCGGTTTTTTTCTATTTTTTTAAGTTAATTTTTAAGAAATTGATAAAA‑AGTTTAAACCTAA‑Tc > 1:6393287/1‑100 (MQ=255) gcAATGACATTAATGAATAATCGTATTTATGGCGGTTCTTTTCTCTTTCTTTTTTTTTATTATTAAACAAAGGATAAAGAGGTTAAAACC‑AA‑TCGCcaaa > 1:2934673/1‑100 (MQ=255) aTGACATTAATGAATAATCGTATTTATGGCGGTTTTTTGCTATTTCTTTATGTTTATTTTTAAGCAATGGGTAAAA‑TGTTTAAAACCAA‑TCTCaaaaaaa > 1:3279133/1‑98 (MQ=255) aTGACATTAATGAATAATCGTATTTATGGCGGTTATTTGCTTTTTTTTTTTGTTTATTTTTAAGCAATGGGTAAAG‑AGTTAAAACCTAATTCCCaaaaaa > 1:2747371/1‑99 (MQ=255) | ATGTGGTACAAAGCGGTAGAAATGATTTAACATTGAATGGTAAAAAAGTGTCAGGCGCCGCAATGACATTAATGAATAATCGTATTTATGGCGGTTATTCGCTATTACTTGATGTTAATTATGAAGCAATGGATAAAG‑TGTTAAAGCCTAA‑TCGCAAAAAGA > NC_007793/380571‑380732 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |