Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,094,628	A→G	N218N (AAT→AAC)	SAUSA300_RS10580 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,094,628	0	A	G	100.0%	12.7 / NA	7	N218N (AAT→AAC)	SAUSA300_RS10580	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (0/7);  total (0/7)

TGGTTCATATATCCCCCAAATATAGCGCATAGGTAGGTTGAATTGACTTTGCGCTTCGTCATCTGTCACAACTAGCTCTAAACGCTTTCCTTTGTCATTTTCGGGTCCCACAGCAATTAATGCTGTTTTGATTTCTGACATATCAATCTTCCTAGTTAACCCAACCAAATCTTTACCAT  >  NC_007793/2094532‑2094710                                                                                                 |                                                                                   aggTTCGTATATTCCCCAAATATAACGGGTAGGTAAGTTGAATTGACTTTGTGCTTCGTCATCTGTCACAACTAACTCTAAACGCTTTCCTTTGTCGttc                                                                                 <  1:3163662/99‑2 (MQ=255)               cccAAATATAACGGGCAGGTAAGTTGAATTGACTTTGTGCTTCGTCATCTGTCACAACTAACTCTAAACGCTTTCCTTTGTCGTTTTCGGGTCCCACAga                                                                   <  1:3280727/100‑2 (MQ=255)                                           ttGACTTTGTGCTTCGTCATCTGTCACAACTAACTCTAAACGCTTTCCTTTGTCGTTTTCGGGTCCCACAGCAATTAATGCTGTTTTGATTTCTGACatt                                       <  1:5055318/100‑2 (MQ=255)                                           ttGACTTTGTGCTTCGTCATCTGTCACAACTAACTCTAAACGCTTTCCTTTGTCGTTTTCGGGTCCCACAGCAATTAATGCTGTTTTGATTTCTGACatt                                       <  1:6585904/100‑2 (MQ=255)                                                                    tcaACTAACTCTAAACGCTTTCCTTTGTCGTTTTCGGGTCCCACAGCAATTAATGCTGTTTTGATTTCTGACATATCAATCTTCCTAGTTAACccaacca              <  1:1842445/99‑1 (MQ=255)                                                                       aCTAACTCTAAACGCTTTCCTTTGTCGTTTTCGGGTCCCACAGCAATTAATGCTGTTTTGATTTCTGACATATCAATCTTCCTAGTTAACCCAACCaaag           <  1:3927973/100‑2 (MQ=255)                                                                                aaaCGCTTTCCTTTGTCGTTTTCGGGTCCCACAGCAATTAATGCTGTTTTGATTTCTGACATATCAATCTTCCTAGTTAACCCAACCAAATCTTTACCAt  <  1:2379792/100‑1 (MQ=255)                                                                                                 |                                                                                   TGGTTCATATATCCCCCAAATATAGCGCATAGGTAGGTTGAATTGACTTTGCGCTTCGTCATCTGTCACAACTAGCTCTAAACGCTTTCCTTTGTCATTTTCGGGTCCCACAGCAATTAATGCTGTTTTGATTTCTGACATATCAATCTTCCTAGTTAACCCAACCAAATCTTTACCAT  >  NC_007793/2094532‑2094710

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑