Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,094,008	G→T	A425E (GCA→GAA)	SAUSA300_RS10580 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,094,008	0	G	T	100.0%	16.4 / NA	8	A425E (GCA→GAA)	SAUSA300_RS10580	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (0/8);  total (0/8)

TTTAATTCACTGAATAGCGCTTTCTCTCTTGTTATACCACCTAATTTTTCAACATCATTTGGTGTTGCTTCAATCCATCGACCATTCCAATATCTACGCAAGACAGCAACATCAGGGTTACTTGTATCATACCAAAGCGTATCAT  >  NC_007793/2093942‑2094086                                                                   |                                                                               tttAATTCACTGAATAGCGCTTTCTCTCTTGTTATACCACCTAATTTTTCAACATCATTTGGTGTTTCTTCAATCCATCGACCATTCCAATATCTACGCa                                               <  1:1767519/100‑1 (MQ=255)           tGAATAGCGCTTTCTCTCTTGTTATACCACCTAATTTTTCAACATCATTTGGTGTTTCTTCAATCCATCGACCATTCCAATATCTACGCAAGACAGCAAc                                     <  1:302373/100‑1 (MQ=255)              ctcttccgatctctcTTGTTATACCACCTAATTTTTCAACATCATTTGGTGTTTCTTCAATCCATCGACCATTCCAATATCTACGCAAGACAGCAACATc                                  <  1:2829976/91‑1 (MQ=255)                    tccgatctctTGTCATACCACCTAATTTTTCAACATCATTTGGTGTTTCTTCAATCCATCGACCATTCCAATATCTACGCAAGACAGCAACATCAgcatg                            <  1:6578653/95‑5 (MQ=255)                    cttccgatctTGTTTTACCACCTAATTTTTCAACATCATTTGGTGTTTCTTCAATCCATCGACCATTCCAATATCTACGCAAGACAGCAACATCAGGGtt                            <  1:61613/93‑1 (MQ=255)                    ccgatctcctTGTTATACCACCTAATTTTTCAACATCATTTGGTGTTTCTTCAATCCATCGACCATTCCAATATCTACGCAAGACAGCAACATCAGGGtt                            <  1:997273/92‑1 (MQ=255)                    cTTTCTCTCTTGTTATACCACCTAATTTTTCAACATCATTTGGTGTTTCTTCAATCCATCGACCATTCCAATATCTACGCAAGACAGCAACATCAGGGtt                            <  1:3988214/100‑1 (MQ=255)                                              ttttCAACATCATTTGGTGTTTCTTCAATCCATCGACCATTCCAATATCTACGCAAGACAGCAACATCAGGGTTACTTGTATCATACCAAAGCATATCAt  <  1:4591016/100‑1 (MQ=255)                                                                   |                                                                               TTTAATTCACTGAATAGCGCTTTCTCTCTTGTTATACCACCTAATTTTTCAACATCATTTGGTGTTGCTTCAATCCATCGACCATTCCAATATCTACGCAAGACAGCAACATCAGGGTTACTTGTATCATACCAAAGCGTATCAT  >  NC_007793/2093942‑2094086

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑