Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,093,733	T→G	K517Q (AAA→CAA)	SAUSA300_RS10580 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,093,733	0	T	G	100.0%	13.1 / NA	7	K517Q (AAA→CAA)	SAUSA300_RS10580	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (2/5);  total (2/5)

GAAATGGCGATTTTGACATCTTCTACATCTGTATAAACATCTTGTAATTTCTTTCTATACTCAAGAAATAAAGTTTTTGTATCTACCAACCGACCAATCGTTGCAGTTTCGGGTGTCATAGATTCTAAATTATTTTTAATTTGATTATAAACATCAATCACAGCGTCTA  >  NC_007793/2093657‑2093825                                                                             |                                                                                             gAAATGGCGATTTTGACATCTTCTACATCTGTATAAACATCTTGTAATTTCTTTCTATACTCAAGAAATAAAGCTTGTGTATCTACCAACTGACCagatc                                                                       >  1:1275589/1‑96 (MQ=255)      ggCGATTTTGACATCTTCTACATCTGTATAAACATCTTGTAATTTCTTTCTATACTCAAGAAATAAAGCTTGTGTATCTACCAACCGACCAATCGTTGCa                                                                  <  1:4608635/100‑1 (MQ=255)                    cttctACATCTGTATAAACATCTTGTAATTTCTTTCTATACTCAAGAAATAAAGCTTGTGTATCTACCAACCGACCAATCGTTGCAGTTTCGGGTGTCAt                                                    <  1:1845993/100‑1 (MQ=255)                    cttctACATCTGTATAAACATCTTGTAATTTCTTTCTATACTCAAGAAATAAAGCTTGTGTATCTACCAACCGACCAATCGTTGCAGTTTCGGGTGTCAt                                                    <  1:2846420/100‑1 (MQ=255)                    cttctACATCTGTATAAACATCTTGTAATTTCTTTCTATACTCAAGAAATAAAGCTTGTGTATCTACCAACCGACCAATCGTTGCAGTTTCGGGTGTCAt                                                    <  1:3884039/100‑1 (MQ=255)                           aTCTGGATAAACATCTTGTAATTTCTTGCTATACTCAAGAAATAAAGCTTGTGTATCTACCAACCGACCAATCGTTGCAGTTTGGGGTGTCATAGATTCt                                             <  1:3394096/100‑1 (MQ=255)                                                                      aaaGCTTGTGTATCTACCAACCGACCAATCGTTGCAGTTTCGGGTGTCATAGATTCTAAATTATTTTTAATTTGATTATAAACATCAATCACAGCGTCTa  >  1:3925406/1‑100 (MQ=255)                                                                             |                                                                                             GAAATGGCGATTTTGACATCTTCTACATCTGTATAAACATCTTGTAATTTCTTTCTATACTCAAGAAATAAAGTTTTTGTATCTACCAACCGACCAATCGTTGCAGTTTCGGGTGTCATAGATTCTAAATTATTTTTAATTTGATTATAAACATCAATCACAGCGTCTA  >  NC_007793/2093657‑2093825

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑