Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,093,013	G→A	L757L (CTG→TTG)	SAUSA300_RS10580 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,093,013	0	G	A	100.0%	47.6 / NA	18	L757L (CTG→TTG)	SAUSA300_RS10580	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (1/17);  total (1/17)

GATTGGTTGCATTAAACTCTTCTTTAGTAGTTCTTAATTTGATTTCCTTACCATTTTGTATAATTTGAGAACCATAGCGAGTCAGTGTTCTCCTCTGTGCATCTGTGCTTTCTTTGACCTTGTTGTCTGTATAAGCATTAGCTTTCTTTTCAGCGTTTCTAGCCTTTAGTTCTGCGTTTT  >  NC_007793/2092929‑2093108                                                                                     |                                                                                                gATTGGTTTCATTAAACTCTTCTTTACTCGTTCTTAATTTCATTTCCTTACCATTTTTTCTAATTTGTTCACCATATCCAGTCAATGTTTTCCTCTGTGc                                                                                  <  1:1406979/100‑1 (MQ=255) gATTGGTTGCATTAAACTCTTCTTTAGTAGTTCTTAATTTGATTTCCTTACCATTTTGTATAATTTGAGAACCATAGCGAGTCAATGTTTTCCTCTGTGc                                                                                  <  1:5595085/100‑1 (MQ=255)  aTTGGTTGCATTAAACTCTTCTTTAGTAGTTCTTAATTTGATTTCCTTACCGTTTTGTATAATTTGAGAACCATAGCGAGTCAATGTTTTCCTCTGTGCa                                                                                 <  1:6160804/100‑1 (MQ=255)   ttGGTTGCATTAAACTCTTCTTTAGTAGTTCTTAATTTGATTTCCTTACCATTTTGTATAATTTGAGAACCATAGCGAGTCAATGTTTTCCTCTGTGCAt                                                                                <  1:2947957/100‑1 (MQ=255)   ttGGTTGCATTAAACTCTTCTTTAGTAGTTCTTAATTTGATTTCCTTACCATTTTGTATAATTTGAGAACCATAGCGAGTCAATGTTTTCCTCTGTGCAt                                                                                <  1:4332590/100‑1 (MQ=255)   ttGGTTGCATTAAACTCTTCTTTAGTAGTTCTTAATTTGATTTCCTTACCATTTTGTATAATTTGAGAACCATAGCGAGTCAATGTTTTCCTCTGTGCAt                                                                                <  1:4516966/100‑1 (MQ=255)      gTTGCATTAAACTCTTCTTTAGTAGTTCTTAATTTGATTTCCTTACCATTTTGTATAATTTGAGAACCATAGCGAGTCAATGTTTTCCTCTGTGCATCtg                                                                             <  1:6141485/100‑1 (MQ=255)       ttGCATTAAACTCTTCTTTAGTAGTTCTTAATTTGGTTTCCTTACCATTTTGTATAATTTGAGAACCATAGCGAGTCAATGTTTTCCTCTGTGCATCtgt                                                                            <  1:2942905/100‑1 (MQ=255)        tGCATTAAACTCTTCTTTAGTAGTTCTTAATTTGATTTCCTTACCATTTTGTATAATTTGAGAACCATAGCGAGTCAATGTTTTCCTCTGTGCATCtgtg                                                                           <  1:1437616/100‑1 (MQ=255)        tGCATTAAACTCTTCTTTAGTAGTTCTTAATTTGATTTCCTTACCATTTTGTATAATTTGAGAACCATAGCGAGTCAATGTTTTCCTCTGTGCATCtggg                                                                           <  1:261234/100‑3 (MQ=255)          cATTAAACTCTTCTTTAGTAGTTCTTAATTTGATTTCCTTACCATTTTGTATAATTTGAGAACCATAGCGAGTCAATGTTTTCCTCTGTGCATCTGTGct                                                                         <  1:3124066/100‑1 (MQ=255)           aTTAAACTCTTCTTTAGTAGTTCTTAATTTGATTTCCTTACCATTTTGTATAATTTGAGAACCATAGCGAGTCAATGTTTTCCTCTGTGCATCTGTGctt                                                                        <  1:316526/100‑1 (MQ=255)           aTTAAACTCTTCTTTAGTAGTTCTTAATTTGATTTCCTTACCATTTTGTATAATTTGAGAACCATAGCGAGTCAATGTTTTCCTCTGTGCATCTGTGcta                                                                        <  1:751462/100‑2 (MQ=255)                                        ttccgatcTTACCATTTTGTATAATTTGAGAACCATAGCGAGTCAATGTTTTCCTCTGTGCATCTGTGCTTTCTTTGACCTTGTTGTCTGTATAAGCAtt                                           <  1:6641725/93‑1 (MQ=255)                                               cTTACCATTTTGTATAATTTGAGAACCATAGCGAGTCAATGTTTTCCTCTGTGCATCTGTGCTTTCTTTGACCTTGTTGTCTGTATAAGCATTAGctttc                                    >  1:1557029/1‑100 (MQ=255)                                                             taATTTGAGAACCATAGCGAGTCAATGTTTTCCTCTGTGCATCTGTGCTTTCTTTGACCTTGTTGTCTGTATAAGCATTAGCTTTCTTTTCAGCGTTTCt                      <  1:3625361/100‑1 (MQ=255)                                                                    agaACCATAGCGAGCCAATGTTTTCCTCTGTGCATCTGTGCTTTCTTTGACCTTGTTGTCTGTATAAGCATTAGCTTTCTTTTCAGCGTTTCTAGCCttt               <  1:4972805/100‑1 (MQ=255)                                                                                 gTCAATGTTTTCCTCTGTGCATCTGTGCTTTCTTTGACCTTGTTGTCTGTATAAGCATTAGCTTTCTTTTCAGCGTTTCTAGCCTTTAGTTCTGCgtttt  <  1:2669548/100‑1 (MQ=255)                                                                                     |                                                                                                GATTGGTTGCATTAAACTCTTCTTTAGTAGTTCTTAATTTGATTTCCTTACCATTTTGTATAATTTGAGAACCATAGCGAGTCAGTGTTCTCCTCTGTGCATCTGTGCTTTCTTTGACCTTGTTGTCTGTATAAGCATTAGCTTTCTTTTCAGCGTTTCTAGCCTTTAGTTCTGCGTTTT  >  NC_007793/2092929‑2093108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑