Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	376,231	T→G	S200R (AGT→CGT)	SAUSA300_RS01715 ←	NADH‑dependent flavin oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	376,231	0	T	G	100.0%	38.4 / NA	17	S200R (AGT→CGT)	SAUSA300_RS01715	NADH‑dependent flavin oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (2/15);  total (2/15)

AAAATAAAATTGTCAGGTGCTTCTTTATCAATCACTTCTTGTACCGCACGCATAACTTCTAAACATAATCTTGCACTATTTTTTAATGAGTCGGCACCGTAATGGTCTGTACGTTTATTCGAAAAAGTTGAGAAAAATGTTTGAATCAGCAAACGTTGTGCAATCGAAATT  >  NC_007793/376155‑376325                                                                             |                                                                                               aaaataaaatTGTCAGGTGCTTCTTTATCAATCACTTCTTGTACCGCACGCATAACTTCTAAACATAATCTTGCACGATTTTTTAATGAGTCGGCACCGt                                                                         <  1:2335177/100‑1 (MQ=255)      aaaatTGTCAGGTGCTTCTTTATCAATCACTTCTTGTACCGCACGCATAACTTCTAAACATAATCTTGCACGATTTTTTAATGAGTCGGCACCGTAATgg                                                                    <  1:5967588/100‑1 (MQ=255)          ttGTCAGGTGCTTCTTTATCAATCACTTCTTGTACCGCACGCATAACTTCTAAACATAATCTTGCACGATTTTTTAATGAGTCGGCACCGTAATGGTCTg                                                                <  1:5914758/100‑1 (MQ=255)           tGTCAGGTGCTTCTTTATCAATCACTTCTTGTACCGCACGCATAACTTCTAAACATAATCTTGCACGATTTTTTAATGAGTCGGCACCGTAATGGTCTga                                                               <  1:2782483/100‑2 (MQ=255)           tGTCAGGTGCTTCTTTATCAATCACTTCTTGTACCGCACGCATAACTTCTAAACATAATCTTGCACGATTTTTTAATGAGTCGGCACCGTAATGGTCTGt                                                               <  1:4137962/100‑1 (MQ=255)           tGTCAGGTGCTTCTTTATCAATCACTTCTTGTACCGCACGCATAACTTCTAAACATAATCTTGCACGATTTTTTAATGAGTCGGCACCGTAATGGTCTGt                                                               <  1:81790/100‑1 (MQ=255)           tGTCAGGTGCTTCTTTATCAATCACTTCTTGTACCGCACGCATAACTTCTAAACATAATCTTGCACGATTTTTTAATGAGTCGGCACCGTAATGGTCTGt                                                               <  1:596506/100‑1 (MQ=255)           tGTCAGGTGCTTCTTTATCAATCACTTCTTGTACCGCACGCATAACTTCTAAACATAATCTTGCACGATTTTTTAATGAGTCGGCACCGTAATGGTCTGt                                                               <  1:3760860/100‑1 (MQ=255)           ccgatcttGCTTCTTTATCAATCACTTCTTGTACCGCACGCATAACTTCTAAACATAATCTTGCACGATTTTTTAATGAGTCGGCACCGTAATGGTCTGt                                                               <  1:1301693/93‑1 (MQ=255)            gTCAGGTGCTTCTTTATCAATCACTTCTTGTTCCGCACGCATAACTTCTAAACATAATCTTGCACGATTTTTTAATGAGTCGGCACCGTAATGGTCTGtt                                                              <  1:6638142/100‑2 (MQ=255)             tCAGGTGCTTCTTTATCAATCACTTCTTGTACCGCACGCATAACTTCTAAACATAATCTTGCACGATTTTTTAATGAGTCGGCACCGTAATGGTCTGTAc                                                             <  1:5981678/100‑1 (MQ=255)                 gTGCTTCTTTATCAATCACTTCTTGTACCGCACGCATAACTTCTAAACATAAGCTTGCACGATTTTTTAATGAGTCGGCACCGTAATGGTCTGTACGttt                                                         <  1:5684923/100‑1 (MQ=255)                               atcaCTTCTTGTACCGCACGCATAACTTCTAAACATAATCTTGCACGATTTTTTAATGAGTCGGCACCGTAATGGTCTGTACGTTTATTCGAAAAAGTTg                                           <  1:519766/100‑1 (MQ=255)                                           aCCGCACGCATAACTTCTAAACATAATCTTGCACGATTTTTTAATGAGTCGGCACCGTAATGGTCTGTACGTTTATTCGAAAAAGTTGAGAAAAATGttt                               >  1:4017201/1‑100 (MQ=255)                                                             aaaCATAATCTTGCACGATTTTTTAATGAGTCGGCACCGTAATGGTCTGTACGTTTATTCGAAAAAGTTGAGAAAAATGTTTGAATCAGCAAACGTtgtg             <  1:3665908/100‑1 (MQ=255)                                                                   aaTCTTGCACGATTTTTTAATGAGTCGGCACCGTAATGGTCTGTACGTTTATTCGAAAAAGTTGAGAAAAATGTTTGAATCAGCAAACGTTGTGCAATCg       <  1:1165439/100‑1 (MQ=255)                                                                        tGCACGATTTTTTAATGAGTCGGCACCGTAATGGTCTGTACGTTTATTCGAAAAAGTTGAGAAAAATGTTTGAATCAGCAAACGTTGTGCAATCGAAAtt  >  1:3226376/1‑100 (MQ=255)                                                                             |                                                                                               AAAATAAAATTGTCAGGTGCTTCTTTATCAATCACTTCTTGTACCGCACGCATAACTTCTAAACATAATCTTGCACTATTTTTTAATGAGTCGGCACCGTAATGGTCTGTACGTTTATTCGAAAAAGTTGAGAAAAATGTTTGAATCAGCAAACGTTGTGCAATCGAAATT  >  NC_007793/376155‑376325

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑