Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	376,057	C→T	D258N (GAC→AAC)	SAUSA300_RS01715 ←	NADH‑dependent flavin oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	376,057	0	C	T	100.0%	42.8 / NA	17	D258N (GAC→AAC)	SAUSA300_RS01715	NADH‑dependent flavin oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/13);  total (4/13)

GTACGAGACGTATTTTGATAAATATGTCGACCCCAGCTAGCGATTGCTAAGTATTGGATGTCTGAAACGTCCATGACCCAATTGATTAATTGGTTGAACTCGTCAATGGTATATCCTAAATCACTGCCTCTGGTTTCTTCTGGCGTTGCTC  >  NC_007793/375996‑376146                                                              |                                                                                          gtACGAGACGTATTTTGATAAATATGTCGACCCCAGCTAGCGATTGCTAAGTATTGGATGTTTGAAACGTCCATGACCCAATTGATTAATTGGTTGAACt                                                     >  1:1183279/1‑100 (MQ=255)             ttttGATAAATATGTCGACCCCAGCTAGCGATTGCTAAGTATTGGATGTTTGAAACGTCCATGACCCAATTGATTAATTGGTTGAACTCGTCAATGGtat                                         <  1:1487819/100‑1 (MQ=255)             ttttGATAAATATGTCGACCCCAGCTAGCGATTGCTAAGTATTGGATGTTTGAAACGTCCATGACCCAATTGATTAATTGGTTGAACTCGTCAATGGtat                                         <  1:456334/100‑1 (MQ=255)             ttttGATAAATATGTCGACCCCAGCTAGCGATTGCTAAGTAGTGGATGTTTGAAACGTCCATGACCCAATTGATTAATTGGTTGAACTCGTCAATGGtat                                         <  1:251048/100‑1 (MQ=255)                              gccccAGCTAGCGATTGCTAAGGATTGGATGTTTGAAACGTCCATGACCCAATTGATTAATTGGTTGAACTCGTCAATGGTATATCCTAAATCACTGCct                        >  1:5023004/2‑100 (MQ=255)                                 ccAGCTAGCGATTGCTAAGTATTGGATGTTTGAAACGTCCATGACCCAATTGATTAATTGGTTGAACTCGTCAATGGTATATCCTAAATCACTGCctcaa                     <  1:3544850/100‑3 (MQ=255)                                    gCTAGCGATTGCTAAGTATTGGATGTTTGAAACGTCCATGACCCAATTGATTAATTGGTTGAACTCGTCAATGGTATATCCTAAATCACTGCCTCTGGtt                  <  1:2167167/100‑1 (MQ=255)                                              gCTAAGTATTGGATGTTTGAAACGTCCATGACCCAATTGATTAATTGGTTGAACTCGTCAATGGTATATCCTAAATCACTGCCTCTGGTTTCTTCTGGCg        >  1:6565067/1‑100 (MQ=255)                                               cTAAGTATTGGATGTTTGAAACGTCCATGACCCAATTGATTAATTGGTTGAACTCGTCAATGGTATATCCTAAATCACTGCCTCTGGTTTCTTCTGGCGt       <  1:1260656/100‑1 (MQ=255)                                               cTAAGTATTGGATGTTTGAAACGTCCATGACCCAATTGATTAATTGGTTGAACTCGTCAATGGTATATCCTAAATCACTGCCTCTGGTTTCTTCTGGCGt       <  1:3091728/100‑1 (MQ=255)                                                tAAGTATTGGATGTTTGAAACGTCCATGACCCAATTGATTAATTGGTTGAACTCGTCAATGGTATATCCTAAATCACTGCCTCTGGTTTCTTCTGGCGta      <  1:892087/100‑2 (MQ=255)                                                tAAGTATTGGATGTTTGAAACGTCCATGACCCAATTGATTAATTGGTTGAACTCGTCAATGGTATATCCGAAATCACTGCCTCTGGTTTCTTCTGGCGta      <  1:137000/100‑2 (MQ=255)                                                 aaGTATTGGATGTTTGAAACGTCCATGACCCAATTGATTAATTGGTTGAACTCGTCAATGGTATATCCTAAATCACTGCCTCTGGTTTCTTCTGGCGtat     <  1:3682363/100‑3 (MQ=255)                                                 aaGTATTGGATGTTTGAAACGTCCATGACCCAATTGATTAATTGGTTGAACTCGTCAATGGTATATCCTAAATCACTGCCTCTGGTTTCTTCTGGCGTTg     >  1:2224989/1‑100 (MQ=255)                                                 aaGTATTGGATGTTTGAAACGTCCATGACCCAATTGATTAATTGGTTGAACTCGTCAATGGCATATCCTAAATCACTGCCTCTGGTTTCTTCTGGCGtaa     <  1:5609040/100‑3 (MQ=255)                                                  aGTATTGGATGTTTGAAACGTCCATGACCCAATTGATTAATTGGTTGAACTCGTCAATGGTATATCCTAAATCACTGCCTCTGGTTTCTTCTGGCGtcgg    <  1:2726414/100‑4 (MQ=255)                                                    tATTGGATGTTTGAAACGTCCATGACCCAATTGATTAATTGGTTGAACTCGTCAATGGTATATCCTAAATCACTGCCTCTGGTTTCTTCTGGCGTTGCta  <  1:4072113/100‑2 (MQ=255)                                                              |                                                                                          GTACGAGACGTATTTTGATAAATATGTCGACCCCAGCTAGCGATTGCTAAGTATTGGATGTCTGAAACGTCCATGACCCAATTGATTAATTGGTTGAACTCGTCAATGGTATATCCTAAATCACTGCCTCTGGTTTCTTCTGGCGTTGCTC  >  NC_007793/375996‑376146

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑