Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,088,904	C→T	G5S (GGT→AGT)	SAUSA300_RS10540 ←	staphylokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,088,904	0	C	T	100.0%	54.5 / NA	22	G5S (GGT→AGT)	SAUSA300_RS10540	staphylokinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (0/22);  total (0/22)

TCCTTTGTCGAATGAACTTGATGCACTTACCTCATTAGTAATTGAAGAAAATGAGAATAATAACAATAAAACAGTTAAAAATAATAAACCTCTTTTGAGCATGGCGCTTCCTCCAAATATAATATATTTGTTTTTATTTATCTCTTTTTATAGCTAACACCCTTATTTAAGATG  >  NC_007793/2088815‑2088988                                                                                          |                                                                                     tCCTTTGTCGAATGAACTTGATGCACTTACCTCATTAGTAATTGAAGAAAATGAGAATAATAACAATAAAACAGTTAAAAATAATAAACTTCTTTTGAGc                                                                            <  1:5706575/100‑1 (MQ=255) tCCTTTGTCGAATGAACTTGATGCACTTACCTCATTAGTAATTGAAGAAAATGAGAATAATAACAATAAAACAGTTAAAAATAATAAACTTCTTTTGAGc                                                                            <  1:480336/100‑1 (MQ=255)   cTTTGTCGAATGAACTTGATGCACTTTCCTCATTAGTAATTGAAGAAAATGAGAATAATAACAATAAAACAGTTAAAAATAATAAACTTCTTTTGAGCAt                                                                          <  1:6049478/100‑1 (MQ=255)   cTTTGTCGAATGAACTTGATGCACTTACCTCATTAGTAATTGAAGAAAATGAGAATAATAACAATAAAACAGTTAAAAATAATAAACTTCTTTTGAGCaa                                                                          <  1:5495863/100‑2 (MQ=255)   cTTTGTCGAATGAACTTGATGCACTTACCTCATTAGTAATTGAAGAAAATGAGAATAATAACAATAAAACAGTTAAAAATAATAAACTTCTTTTGAGCAt                                                                          <  1:702957/100‑1 (MQ=255)    tttGTCGAATGAACTTGATGCACTTACCTCATTAGTAATTGAAGAAAATGAGAATAATAACAATAAAACAGTTAAAAATAATAAACTTCTTTTGAGCATg                                                                         <  1:3633391/100‑1 (MQ=255)     ttGTCGAATGAACTTGATGCACTTACCTCATTAGTAATTGAAGAAAATGAGAATAATAACAATAAAACAGTTAAAAATAATAAACTTCTTTTGAGCATgg                                                                        <  1:4097452/100‑1 (MQ=255)      tGTCGAATGAACTTGATGCACTTACCTCATTAGTAATTGAAGAAAATGAGAATAATAACAATAAAACAGTTAAAAATAATAAACTTCTTTTGAGCATggg                                                                       <  1:2648265/100‑2 (MQ=255)      tGTCGAATGAACTTGATGCACTTACCTCATTAGTAATTGAAGAAAATGAGAATAATAACAATAAAACAGTTAAAAATAATAAACTTCTTTTGAGCATGgc                                                                       <  1:5881350/100‑1 (MQ=255)                   tGATGCACTTACCTCATTAGTAATTGAAGAAAATGAGAATAATAACAATAAAACAGTTAAAAATAATAAACTTCTTTTGAGCATGGCGCTTCCTCCAaat                                                          <  1:3729203/100‑1 (MQ=255)                        cACTTACCTCATTAGTAATTTAAGAAAATGAGAATAATAACAATAAATCAGTTAAAAATAATAAACTTCTTTTGAGCATGGCGCTTCCTCCAaatataat                                                     <  1:1113031/100‑1 (MQ=255)                        cACTTACCTCATTAGTAATTGAAGAAAATGAGAATAATAACAATAAAACAGTTAAAAATAATAAACTTCTTTTGAGCATGGCGCTTCCTCCAaatataat                                                     <  1:3945169/100‑1 (MQ=255)                        cACTTACCTCATTAGTAATTGAAGAAAATGAGAATAATAACAATAAAACAGTTAAAAATAATAAACTTCTTTTGAGCATGGCGCTTCCTCCAaatataat                                                     <  1:655089/100‑1 (MQ=255)                                                        aataatAACAATAAAACAGTTAAAAATAATAAACTTCTTTTGAGCATGGCGCTTCCTCCAAATATAATATATTTGTTTTTATTTATCTCTTTTTATAGCt                     <  1:1045879/100‑1 (MQ=255)                                                        aataatAACAATAAAACAGTTAAAAATAATAAACTTCTTTTGAGCATGGCGCTTCCTCCAAATATAATATATTTGTTTTTATTTATCTCTTTTTATAGCt                     <  1:5621712/100‑1 (MQ=255)                                                         ataataACAATAAAACAGTTAAAAATAATAAACTTCTTTTGAGCATGGCGCTTCCTCCAAATATAATATATTTGTTTTTATTTATCTCTTTTTATAGCTa                    <  1:1702708/100‑1 (MQ=255)                                                                    aaaaCAGTTAAAAATAATAAACTTCTTTTGAGCATGGCGCTTCCTCCAAATATAATATATTTGTTTTTATTTATCTCTTTTTATAGCTAACACCCTTAtt         <  1:4085774/100‑1 (MQ=255)                                                                      aaCAGTTAAAAATAATAAACTTCTTTTGAGCATGGCGCTTCCTCCAAATATAATATATTTGTTTTTATTTATCTCTTTTTATAGCTAACACCCTTATTTa       <  1:5375093/100‑1 (MQ=255)                                                                      aaCAGTTAAAAATAATAAACTTCTTTTGAGCATGGCGCTTCCTCCAAATATAATATATTTGTTTTTATTTATCTCTTTTTATAGCTAACACCCTTATTTa       <  1:2756205/100‑1 (MQ=255)                                                                      aaCAGTTAAAAATAATAAACTTCTTTTGAGCATGGCGCTTCCTCCAAATATAATATATTTGTTTTTATTTATCTCTTTTTATAGCTAACACCCTTATTTa       <  1:288264/100‑1 (MQ=255)                                                                           ttAAAAATAATAAACTTCTTTTGAGCATGGCGCTTCCTCCAAATATAATATATTTGTTTTTATTTATCTCTTTTTATAGCTAACACCCTTATTTAAgatg  <  1:5274007/100‑1 (MQ=255)                                                                           ttAAAAATAATAAACTTCTTTTGAGCATGGCGCTTCCTCCAAATATAATATATTTGTTTTTATTTATCTCTTTTTATAGCTAACACCCTTATTTAAgatg  <  1:6153887/100‑1 (MQ=255)                                                                                          |                                                                                     TCCTTTGTCGAATGAACTTGATGCACTTACCTCATTAGTAATTGAAGAAAATGAGAATAATAACAATAAAACAGTTAAAAATAATAAACCTCTTTTGAGCATGGCGCTTCCTCCAAATATAATATATTTGTTTTTATTTATCTCTTTTTATAGCTAACACCCTTATTTAAGATG  >  NC_007793/2088815‑2088988

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑