Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,088,140	A→G	intergenic (‑404/+285)	SAUSA300_RS10535 ← / ← SAUSA300_RS10540	peptidoglycan hydrolase/staphylokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,088,140	0	A	G	100.0%	56.4 / NA	23	intergenic (‑404/+285)	SAUSA300_RS10535/SAUSA300_RS10540	peptidoglycan hydrolase/staphylokinase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (2/21);  total (2/21)

ACTATGCTCTCTATCATAAG‑AAAAAACACCCAGTGACATGCTTGGGTGAACAAGGATAGATGTAAATAGTTGATGCATGTGTAACACATCATAACAAAAAACTAGCCCGAAAGCTAGCTATAACATACAATCTAAAAAGACGTCCCTTGA  >  NC_007793/2088047‑2088196                                                                                               |                                                         ctcttccgatctATCATAAGAAAAAAACACCCAGTGACATGCTTGGGTGAACAAGGATAGATGTAAATAGTTGATGCATGTGTAACACATCATAGCaaaa                                                     <  1:4094286/91‑1 (MQ=255)           ctATCGTAAGAAAAAAACGCCCAGTGACATGCTTGGGTGAACAAGGATAGATGTAAATAGTTGATGCATGTGTAACACATCATAGCAAAAAACTAGCCCg                                           <  1:2704453/100‑1 (MQ=255)                 ttagaaaaaaaCACCCAGTGACATGCTTGGGTGAACAAGGATAGATGTAAATAGTTGATGCATGTGTAACACATCATAGCAAAAAACTAGCCCGAAagct                                     <  1:4334204/95‑1 (MQ=255)                     aaaaaaaCACCCAGTGACATGCATGGGTGAACAAGGATAGATGTAAATAGTTGATGCATGTGTAACACATCATAGCAAAAAACTAGCCCGAAagctagct                                 <  1:948596/99‑1 (MQ=255)                      aaaaaaCACCCAGTGACATGCTTGGGTGAACAAGGATAGATGTAAATAGTTGATGCATGCGTAACACATCATAGCAAAAAACTAGCCCGAAAgctagcta                                <  1:432173/100‑1 (MQ=255)                       aaaaaCACCCAGTGACATGCTTGGGTGAACAAGGATAGATGTAAATAGTTGATGCATGTGTAACACATCATAGCAAAAAACTAGCCCGAAAgctagctag                               <  1:5171156/100‑2 (MQ=255)                              cccAGTGACATGCTTGGGTGAACAAGGATAGATGTAAATAGTTGATGCATGTGTAACACATCATAGCAAAAAACTAGCCCGAAAGCTAGCTATAACATAc                        <  1:3982055/100‑1 (MQ=255)                                    gACATGCTTGGGTGAACAAGGATAGATGTAAATAGTTGATGCATGTGTAACACATCATAGCAAAAAACTAGCCCGAAAGCTAGCTATAACATACAATCTa                  >  1:1185439/1‑100 (MQ=255)                                    gACATGCTTGGGTGAACAAGGATAGATGTAAATAGTTGATGCATGTGTAACACATCATAGCAAAAAACTAGCCCGAAAGCTAGCTATAACATACAATCTa                  <  1:1710712/100‑1 (MQ=255)                                     aCATGCTTGGGTGAACAAGGATAGATGTAAATAGTTGATGCATGTGTAACACATCATAGCAAAAAACTAGCCCGAAAGCTAGCTATAACATACAATCTaa                 >  1:1258501/1‑100 (MQ=255)                                     aCATGCTTGGGTGAACAAGGATAGATGTAAATAGTTGATGCATGTGTAACACATCATAGCAAAAAACTAGCCCGAAAGCTAGCTATAACATACAATCTaa                 <  1:6399588/100‑1 (MQ=255)                                      cATGCTTGGGTGAACAAGGATAGATGTAAATAGTTGATGCATGTGTAACACATCATAGCAAAAAGCTAGCCCGAAAGCTAGCTATAACATACAATCTaca                <  1:152911/100‑3 (MQ=255)                                      cATGCTTGGGTGAACAAGGATAGATGTAAATAGTTGATGCATGTGTAACACATCATAGCAAAAAACTAGCCCGAAAGCTAGCTATAACATACAATCTacc                <  1:5908878/100‑3 (MQ=255)                                       aTGCTTGGGTGAACAAGGATAGATGTAAATAGTTGATGCATGTGTAACACATCATAGCAAAAAACTAGCCCGAAAGCTAGCTATAACATACAATCTaaaa               <  1:851458/100‑1 (MQ=255)                                                 gAACAAGGATAGATGTAAATAGTTGATGCATGTGTAACACATCATAGCAAAAAACTAGCCCGAAAGCTAGCTATAACATACAATCTAAAAAGACGTcctt     <  1:1103536/100‑3 (MQ=255)                                                  aaCAAGGATAGATGTAAATAGTTGATGCATGTGTAACACATCATAGCAAAAAACTAGCCCGAAAGCTAGCTATAACATACAATCTAAAAAGACGTCCttt    <  1:5279721/100‑1 (MQ=255)                                                   aCAAGGATAGATGTAAATAGTTGATGCATGTGTAACACATCATAGCAAAAAACTAGCCCGAAAGCTAGCTATAACATACAATCTAAAAAGACGTCCTTTg   <  1:3397233/100‑1 (MQ=255)                                                   aCAAGGATAGATGTAAATAGTTGATGCATGTGTAACACATCATAGCAAAAAACTAGCCCGAAAGCTAGCTATAACATACAATCTAAAAAGACGTCCTTTg   <  1:498459/100‑1 (MQ=255)                                                   aCAAGGATAGATGTAAATAGTTGATGCATGTGTAACACATCATAGCAAAAAACTAGCCCGAAAGCTAGCTATAACATACAATCTAAAAAGACGTCCTTTg   <  1:5191272/100‑1 (MQ=255)                                                   aCAAGGATAGATGTAAATAGTTGATGCATGTGTAACACATCATAGCAAAAAACTAGCCCGAAAGCTAGCTATAACATACAATCTAAAAAGACGTCCTTTg   <  1:6099085/100‑1 (MQ=255)                                                   aCAAGGATAGATGTAAATAGTTGATGCATGTGTAACACATCATAGCAAAAAACTAGCCCGAAAGCTAGCTATAACATACAATCTAAAAAGACGTCCTTTg   <  1:6224370/100‑1 (MQ=255)                                                   aCAAGGATAGATGTAAATAGTTGATGCATGTGTAACACATCATAGCAAAAAACTAGCCCGAAAGCTAGCTATAACATACAATCTAAAAAGACGTCCTTTg   <  1:2814565/100‑1 (MQ=255)                                                    cgaGGATAGATGTAAATAGTTGATGCATGTGTAACACATCATAGCAAAAAACTAGCCCGAAAGCTAGCTATAACATACAATCTAAAAAGACGTCCTTTGa  <  1:2948823/98‑1 (MQ=255)                                                                                               |                                                         ACTATGCTCTCTATCATAAG‑AAAAAACACCCAGTGACATGCTTGGGTGAACAAGGATAGATGTAAATAGTTGATGCATGTGTAACACATCATAACAAAAAACTAGCCCGAAAGCTAGCTATAACATACAATCTAAAAAGACGTCCCTTGA  >  NC_007793/2088047‑2088196

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑