Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,088,031	A→G	intergenic (‑295/+394)	SAUSA300_RS10535 ← / ← SAUSA300_RS10540	peptidoglycan hydrolase/staphylokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,088,031	0	A	G	100.0%	42.5 / NA	20	intergenic (‑295/+394)	SAUSA300_RS10535/SAUSA300_RS10540	peptidoglycan hydrolase/staphylokinase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (1/19);  total (1/19)

GGCTATATAGTTCACTCCTACTATATACAGTTAATTATAACATAAAAAGCACCCCGTAAACTATTATACGGGAATGCTAAAGTCATATATACTACGAGGGAGTAGTATGAAAACTATGCTCTCTATCATAAG‑AAAAAACACCCAGTGACATGCTTGGGTGAACAAGGATAGATGTAAATAGTTGATGCATGT  >  NC_007793/2087935‑2088126                                                                                                 |                                                                                                 ggCTATATAGTTCACTCCTACTATATACACTTAATTATAACATAAAAAGCACCCCGTAAACTATTATACGGGAATGCTAAAGTCATATATACTACGgggg                                                                                               <  1:6131401/100‑1 (MQ=255)    gatataGTGCACTCCTGCTATATACACTTAATTATAACATAAAAAGGACCCCGTAAACTATTAGACGGGAATGCTAAAGTCATATATACTACGGGGGagt                                                                                            <  1:3814821/99‑1 (MQ=38)     atataGTTCACTCCTACTATATACACTTAATTATAACATAAAAAGCACCCCGTAAACTATTATACGGGAATGCTAAAGTCATATATACTACGGGGGagta                                                                                           <  1:3119567/100‑1 (MQ=255)                    actaTATACACTTAATTATAACATAAAAAGCACCCCGTAAACTATTATACGGGAATGCTAAAGTCATATATACTACGGGGGAGTAGTATGAAAACTagag                                                                            >  1:127455/1‑97 (MQ=255)                                             aaaaGCACCCCGTAAACTATTATACGGGAATGCTAAAGTCATATATACTACGGGGGAGTAGTATGAAAACTATGCTCTCTATCATAAGAAAAAAACAccc                                                   <  1:1866495/100‑1 (MQ=255)                                             aaaaGCACCCCGTAAACTATTATACGGGAATGCTAAAGTCATATATACTACGGGGGAGTAGTATGAAAACTATGCTCTCTATCATAAGAAAAAAACAccc                                                   <  1:4426058/100‑1 (MQ=255)                                               aaGCACCCCGTAAACTGTTATGCGGGAATGGTAAAGTCATATATACTACGGGGGAGTAGTATGAAAACTATGCTCTCTATCATAAG‑AAAAAAAACCCAGt                                                <  1:3947062/100‑1 (MQ=255)                                                      ccGTAAACTATTATACGGGAATGCTAAAGTCATATATACTGCGGGGGAGTAGTATGAAAACTATGCTCTCTATCATAAG‑AAAAAAAACCCAGTGACATGc                                         <  1:537606/100‑1 (MQ=255)                                                      ccGTAAACTATTATACGGGAATGCTAAAGTCATATATACTACGGGGGAGTAGTATGAAAACTATGCTCTCTATCATAAGAAAAAAACACCCAGTGACATg                                          <  1:5619602/100‑1 (MQ=255)                                                       cGTAAACTATTATACGGGAATGCTAAAGTCATATATACTACGGGGGAGTAGTATGAAAACCATGCTCTCTATCATAAGAAAAAAACACCCAGTGACATGc                                         <  1:1445654/100‑1 (MQ=255)                                                                               aaaGTCATATATACTACGGGGGAGTAGTATGAAAACTATGCTCTCTATCATAAGAAAAAAACACCCAGTGACATGCTTGGGTGAACAAGGATAGATGTaa                 <  1:5583925/100‑1 (MQ=255)                                                                                              acGGGGGAGTAGTATGAAAACTATGCTCTCTATCATAAGAAAAAAACACCCAGTGACATGCTTGGTTGAACAAGGATAGATGTAAATAGTTGATGCAtgt  <  1:6144547/100‑1 (MQ=255)                                                                                              acGGGGGAGTAGTATGAAAACTATGCTCTCTATCATAAGAAAAAAACACCCAGTGACATGCTTGGGTGAACAAGGATAGATGTAAATAGTTGATGCaggg  <  1:280926/100‑4 (MQ=255)                                                                                              acGGGGGAGTAGTATGAAAACTATGCTCTCTATCATAAGAAAAAAACACCCAGTGACATGCTTGGGTGAACAAGGATAGATGTAAATAGTTGATGCAtgt  <  1:4306776/100‑1 (MQ=255)                                                                                              acGGGGGAGTAGTATGAAAACTATGCTCTCTATCATAAGAAAAAAACACCCAGTGACATGCTTGGGTGAACAAGGATAGATGTAAATAGTTGATGCAtgt  <  1:4768279/100‑1 (MQ=255)                                                                                              acGGGGGAGTAGTATGAAAACTATGCTCTCTATCATAAGAAAAAAACACCCAGTGACATGCTTGGGTGAACAAGGATAGATGTAAATAGTTGATGCAtgt  <  1:3083999/100‑1 (MQ=255)                                                                                              acGGGGGAGTAGTATGAAAACTATGCTCTCTATCATAAGAAAAAAACACCCAGTGACATGCTTGGGTGAACAAGGATAGATGTAAATAGTTGATGCAtgt  <  1:6071262/100‑1 (MQ=255)                                                                                              acGGGGGAGTAGTATGAAAACTATGCTCTCTATCATAAGAAAAAAACACCCAGTGACATGCTGGGGTGAACAAGGATAGATGTAAATAGTTGATGCAtgt  <  1:5807678/100‑1 (MQ=255)                                                                                              acGGGGGAGTAGTATGAAAACTATGCTCTCTATCATAAGAAAAAAACACCCAGTGACAGGCTTGGGTGAACAAGGATAGATGTAAATAGTTGATGCAtgt  <  1:3797526/100‑1 (MQ=255)                                                                                              acGGGGGAGTAGTATGAAAACTATGCTCTCTATCATAAGAAAAAAACACCCAGTGACAGGCTTGGGTGAACAAGGATAGATGTAAATAGTTGATGCAtgt  <  1:6624402/100‑1 (MQ=255)                                                                                              acGGGGGAGTAGTACGAAAACTATGCTCTCTATCATAAGAAAAAAACACCCAGTGACATGCTTGGGTGAACAAGGATAGATGTAAATAGTTGATGCAtgt  <  1:4912068/100‑1 (MQ=255)                                                                                                 |                                                                                                 GGCTATATAGTTCACTCCTACTATATACAGTTAATTATAACATAAAAAGCACCCCGTAAACTATTATACGGGAATGCTAAAGTCATATATACTACGAGGGAGTAGTATGAAAACTATGCTCTCTATCATAAG‑AAAAAACACCCAGTGACATGCTTGGGTGAACAAGGATAGATGTAAATAGTTGATGCATGT  >  NC_007793/2087935‑2088126

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑