Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,087,514	G→A	R75C (CGT→TGT)	SAUSA300_RS10535 ←	peptidoglycan hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,087,514	0	G	A	100.0%	72.6 / NA	31	R75C (CGT→TGT)	SAUSA300_RS10535	peptidoglycan hydrolase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (2/29);  total (2/29)

ACTTACCAAAACTGCTTATTCTATTACCTGCCTTGTCTACCTCTCCTGTCGCTATATAACGACGTTGTCCACTATTAGCAATATAAGTAATCCATCTATAGCCATTAATGCAATATGCGCCGTCATATTTGATAGTTGCGTTATTGGGTAATACACC  >  NC_007793/2087454‑2087610                                                             |                                                                                                 tcTTACCAGAACTACTTATTCTATTACCTGCCTTGTCTACCTCTCCTGTCGCTATATAACAACGTTGTCCATTATTAGCAATATAAGTAATCCATCtatg                                                           <  1:5484705/99‑2 (MQ=18)      tcttccgatcTTATTCTATTACCTGCCTTGTCTACCTCTCCTGTCGCTATATAACAACGTTGTCCATTATTAGCAATATAAGTAATCCATCTATAGCCAt                                                      <  1:6491606/91‑1 (MQ=18)        ttccgatcTTATTCTATTACCTGCCTTGTCTACCTCTCCTGTCGCTATATAACAACGTTGTCCATTATTAGCAATATAAGTAATCCATCTATAGCCATTa                                                    <  1:1897204/93‑1 (MQ=18)        tctactactTATTCTATTACCTGCCTTGTCTACCTCTCCTGTCGCTATATAACAACGTTGTCCATTATTAGCAATATAAGTAATCCATCTATAGCCATTa                                                    <  1:2526755/97‑1 (MQ=18)        cgatctacTTACTCTATTACCTGCCTTGTCTACCTCTCCTGTCGCTATATAACAACGTTGTCCATTATTAGCAATATAAGTAATCCATCTATAGCCATTa                                                    <  1:4246491/93‑1 (MQ=18)        cgatcTGCTTATTCTATTACCTGCCTTGTCTACCTCTCCTGTCGCTATATAACAACGTTGTCCATTATTAGCAATATAAGTAATCCATCTATAGCCATTa                                                    <  1:3160112/96‑1 (MQ=255)        ccgatctattATTCTATTACCTGCCTTGTCTACCTCTCCTGTCGCTATATAACAACGTTGTCCATTATTAGCAATATAAGTAATCCATCTATAGCCATTa                                                    <  1:4323084/92‑1 (MQ=18)         tccgatcTTATTCTATTACCTGCCTTGTCTACCTCTCCTGTCGCTATATAACAACGTTGTCCATTATTAGCAATATAAGTAATCCATCTATAGCCATTaa                                                   <  1:4913488/94‑1 (MQ=18)               tccgatCTGTTACCTGCCTTGTCTACCTCTCCTGTCGCTATATAACAACGTTGTCCATTATTAGCAATATAAGTAATCCATCTATAGCCATTAATGCAat                                             <  1:3296505/95‑1 (MQ=18)               cTTATTCTATTACCTGCCTTGTCTACCTCTCCTGTCGCTATATAACAACGTTGTCCATTATTAGCAATATAAGTAATCCATCTATAGCCATTAATGCAat                                             <  1:2144556/100‑1 (MQ=255)                tccgatctctACCTGCCTTGTCTACCTCTCCTGTCGCTATATAACAACGTTGTCCATTATTAGCAATATAAGTAATCCATCTATAGCCATTAATGCAata                                            <  1:3886764/91‑1 (MQ=18)                    tCTATTACCTGCCTTGTCTACCTCTCCTGTCGCTATATAACAACGTTGTCCATTATTAGCAATATAAGTAATCCATCTATAGCCATTAATGCAATATgca                                        <  1:6617588/100‑2 (MQ=255)                     cTATTACCTGCCTTGTCTACCTCTCCTGTCGCTATATAACAACGTTGTCCATTATTAGCAATATAAGTAATCCATCTATAGCCATTAATGCAATATgcgc                                       >  1:3752002/1‑100 (MQ=255)                         atcttGCCTTGTCTACCTCTCCTGTCGCTATATAACAACGTTGTCCATTATTAGCAATATAAGTAATCCATCTATAGCCATTAATGCAATATGCGCCGTc                                   <  1:274075/96‑1 (MQ=255)                           ccTGCCTTGTCTACCTCTCCTGTCGCTATATAACAACGTTGTCCATTATTAGCAATATAAGTAATCCATCTATAGCCATTAATGCAATATGCGCCGTCat                                 <  1:641398/100‑1 (MQ=255)                             tGCCTTATCTACCTCTCCTGTCGCTATATAACAACGTTGTCCATTATTAGCAATATAAGTAATCCATCTATAGCCATTAATGCAATATGCGCCGTCatgt                               <  1:4424813/100‑3 (MQ=17)                                cTTGTCTACCTCTCCTGTCGCTATATAACAACGTTGTCCATTATTAGCAATATAAGTAATCCATCTATAGCCATTAATGCAATATGCGCCGTCATATTTg                            <  1:2141144/100‑1 (MQ=255)                                       aCCTTTCCTGTCGCTATATAACAACGTTGTCCATTATTAGCAATATAAGTAATCCATCTATAGCCATTAATGCAATATGCGCCGTCATATTTGATCGTTg                     >  1:3207972/1‑100 (MQ=18)                                        tctctCCTTTCGCTATATAACAACGTTGTCCATTATTAGCAATATAAGTAATCCATCTATAGCCATTAATGCAATATGCGCCGTCATATTTGATCGTTGc                    <  1:1986802/99‑1 (MQ=18)                                        gatctcCTGTTGCTATATAACAACGTTGTCCATTATTAGCAATATAAGTAATCCATCTATAGCCATTAATGCAATATGCGCCGTCATATTTGATCGTTGc                    <  1:1290352/98‑1 (MQ=18)                                         cactcCTTTCCCTAAATGACAACGTTGTCCATTATTAGCCAAGAAAGTAATCCATCTATAGCCATTAATGCAATATGCGCCGTCATATTTGATCGTTGct                   <  1:291149/98‑2 (MQ=255)                                           cttcTGTCGCTATATAACAACGTTGTCCATTATTAGCAATATAAGTAATCCATCTATAGCCATTAATGCAATATGCGCCGTCATATTTGATCGTTGCGtt                 <  1:6102368/97‑1 (MQ=18)                                           ctcCTTTCGCTATATAACAACGCTGTCCATTATTAGCAATATAAGTAATCCATCTATAGCCATTAATACAATATGCGCCGTCATATTTGATCGTTGCGtt                 <  1:442493/100‑1 (MQ=18)                                           ctcCTGTTGCTATATAACAACGTTGTCCATTATTAGCAATATAAGTAATCCATCTATAGCCATTAATGCAATATGCGCCGTCATATTTGATCGTTGCGtt                 <  1:355760/100‑1 (MQ=21)                                           ctcCTGTCGATATATAACAACGTTGTCCATTATTAGCAATATAAGTAATCCATCTATAGCCATTAATGCAATATGCGCCGTCATATTTGATCGTTGCGtt                 <  1:6091706/100‑1 (MQ=21)                                            tcCTGTTGCTATATAACAACGTTGTCCATTATTAGCAATATAAGTAATCCATCTATAGCCATTAATGCAATATGCGCCGTCATATTTGATCGTTGCggtc                <  1:1103465/100‑4 (MQ=18)                                            tcCTGTCGCTATATAACAACGTTGTCCATTATTAGCAATATAAGTAATCCATCTATAGCCATTAATGCAATATGCGCCGTCATATTTGATCGTTGCGTTa                <  1:2743182/100‑1 (MQ=255)                                            tcCTGTCGCTATATAACAACGTTGTCCATTATTAGCAATATAAGTAATCCATCTATAGCCATTAATGCAATATGCGCCGTCATATTTGATCGTTGCGTTa                <  1:6575457/100‑1 (MQ=255)                                             ccTGTCGCTATATAACAACGTTGTCCATTATTGTCAATATAAGTAATCCATCTATAGCCATTAATGCAATATGCGCCGTCATATTTGAgcgttgcgttgg               <  1:6269135/100‑3 (MQ=18)                                             ccTGTCGCTATATAACAACGTTGTCCATTATTAGCAATATAAGTAATCCATCTATAGCCATTAATGCAATATGCGCCGTCATATTTGATCGTTGCGttgt               <  1:5954870/100‑3 (MQ=18)                                             ccTGTCGCTATATAACAACGTTGTCCATTATTAGCAATATAAGTAATCCATCTATAGCCATTAATGCAATATGCGCCGTCATATTTGATCGTTGCGTTag               <  1:4417628/100‑2 (MQ=255)                                                          aacaacGTTGTCCATTATTAGCAATATAAGTAATCCATCTATAGCCATTAATGCAATATGCGCCGTCATATTTGATCGTTGCGTTATTGGGTAATACAcc  <  1:1139326/100‑1 (MQ=255)                                                          aacaacGTTGTCCATTATTAGCAATATAAGTAATCCATCTATAGCCATTAATGCAATATGCGCCGTCATATTTGATCGTTGCGTTATTGGGTAATACAcc  <  1:3198487/100‑1 (MQ=255)                                                             |                                                                                                 ACTTACCAAAACTGCTTATTCTATTACCTGCCTTGTCTACCTCTCCTGTCGCTATATAACGACGTTGTCCACTATTAGCAATATAAGTAATCCATCTATAGCCATTAATGCAATATGCGCCGTCATATTTGATAGTTGCGTTATTGGGTAATACACC  >  NC_007793/2087454‑2087610

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑