Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,085,622	T→C	A2A (GCT→GCC)	SAUSA300_RS10520 →	membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,085,622	0	T	C	100.0%	218.1 / NA	84	A2A (GCT→GCC)	SAUSA300_RS10520	membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (77/7);  total (77/7)

TATTTATTTATGAAGGGTGTGTATTGTTATTTATCTAATTTATAGTCTAATTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCTGCACCATTCTTAATGACTAATGCTGATTATGTATGGACGCCTATGACTACAGTTACGTTATTTATTTTGAGTTTGATTATTTTCTTGATATTTATA  >  NC_007793/2085532‑2085718                                                                                           |                                                                                                 aatttatttatGAAGGGTGTGTATTGTTATTTACCTAATTTATAGTCTAATTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCCGCACCATTc                                                                                         >  1:5163377/2‑100 (MQ=255)          tcttaGGGTGTGTATTGTTATTTACCTAATTTATAGTCTAATTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACt                                                                                >  1:6371741/5‑100 (MQ=255)          gtGAAGGGTGTGTATTGTTATTTACCTAATTTATAGTCTAATTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACt                                                                                >  1:6441308/2‑100 (MQ=255)          atGAAGGGTGTGTATTGTTATTTACCTAATTTATAGTCTAATTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACt                                                                                >  1:5582369/1‑100 (MQ=255)          atGAAGGGTGTGTATTGTTATTTACCTAATTTATAGTCTAATTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACt                                                                                >  1:1765905/1‑100 (MQ=255)          atGAAGGGTGTGTATTGTTATTTACCTAATTTATAGTCTAATTGTCTATTTCATCTGCATCATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACt                                                                                >  1:5940493/1‑100 (MQ=255)           tGAAGGGTGTGTATTGTTATTTACCTAATTTATAGTCTAATTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACTa                                                                               >  1:368058/1‑100 (MQ=255)           tGAAGGGTGTGTATTGTTATTTACCTAATTTATAGTCTAATTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACTa                                                                               >  1:5666306/1‑100 (MQ=255)           tGAAGGGTGTGTATTGTTATTTACCTAATTTATAGTCTAATTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACTa                                                                               >  1:1524875/1‑100 (MQ=255)            tcaGGGTGTGTATTGTTATTTACCTAATTTATAGTCTAATTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACTaa                                                                              >  1:4360632/3‑100 (MQ=255)            gAAGGGTGTGTATTGTTATTTACCTAATTTATAGTCTAATTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACTaa                                                                              >  1:558133/1‑100 (MQ=255)            gAAGGGTGTGTATTGTTATTTACCTAATTTATAGTCTAATTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACTaa                                                                              >  1:4756706/1‑100 (MQ=255)            ccaGGGTGTGTATTGTTATTTACCTAATTTATAGTCTAATTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACTaa                                                                              >  1:4163040/3‑100 (MQ=255)             gaGGGTGTGTATTGTTATTTACCTAATTTATAGTCTAATTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACTAAt                                                                             >  1:4207691/2‑100 (MQ=255)             gaGGGTGTGTATTGTTATTTACCTAATTTATAGTCTAATTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACTAAt                                                                             >  1:5463485/2‑100 (MQ=255)               gggTGTGTATTGTTATTTACCTAATTTATAGTCTAATTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCCGCACGATTCTTAATGACTaagtt                                                                           <  1:4073154/100‑4 (MQ=255)                ggTGTGTATTGTTATTTACCTAATTTATAGTCTAATTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACTAATGCt                                                                          >  1:965503/1‑100 (MQ=255)                 ctgtgtATTGTTATTTACCTAATTTATAGTCTAATTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACTAATGCTg                                                                         >  1:2141846/2‑100 (MQ=255)                  tgtgtATTGTTATTTACCTAATTTATAGTCTAATTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCCGCACCATTCTTGATGACTAATGCTGa                                                                        >  1:4982191/1‑100 (MQ=255)                   gtgtATTGTTATTTACCTAATTTATAGTCTAATTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCCGCACCATTCTTACTGACTAATGCTGAt                                                                       >  1:4974925/1‑100 (MQ=255)                   gtgtATTGTTATTTACCTAATTTATAGTCTAATTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACTAATGCTGAt                                                                       >  1:6364453/1‑100 (MQ=255)                   gtgtATTGTTATTTACCTAATTTATAGTCTAATTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACTAATGCTGAt                                                                       >  1:35642/1‑100 (MQ=255)                   gtgtATTGTTATTTACCTAATTTATAGTCTAATTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACTAATGCTGAt                                                                       >  1:3633564/1‑100 (MQ=255)                   gtgtATTGTTATTTACCTAATTTATAGTCTAATTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACTAATGCTGAt                                                                       >  1:4709487/1‑100 (MQ=255)                   gtgtATTGTTATTTACCTAATTTATAGTCTAATTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACTAATGCTGAt                                                                       >  1:3090003/1‑100 (MQ=255)                   gtgtATTGTTATTTACCTAATTTATAGTCTAATTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACTAATGCTGAt                                                                       >  1:160310/1‑100 (MQ=255)                   gtgtATTGTTATTTACCTAATTTATAGTCTAATTGTCTATCTCATCTGCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACTAATGCTGAt                                                                       >  1:4081710/1‑100 (MQ=255)                       aTTGTTATTTACCTAATTTATAGTCTAATTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACTAATGCTGATtatg                                                                   >  1:5444619/1‑100 (MQ=255)                       aTTGTTATTTACCTAATTTATAGTCTAATTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACTAATGCTGATtatg                                                                   >  1:5573341/1‑100 (MQ=255)                         tGTTATTTACCTAATTTATAGTCTAATTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACTAATGCTGATtatgta                                                                 >  1:4066915/1‑100 (MQ=255)                            tatttacCTAATTTATAGTCTAATTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACTAATGCTGATTATGTATgg                                                              <  1:646319/100‑1 (MQ=255)                              cttacCTAATTTATAGTCTAATTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACTAATGCTGATTATGTATGGAc                                                            >  1:5919566/2‑100 (MQ=255)                                tacccaaTTTATAGTCTAATTGTCTACTTCATCTGCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACTAATGCTGATTATGTATGGACGc                                                          >  1:4070221/6‑100 (MQ=255)                                     aaTTTATAGTCTAATTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACTAATGCTGATTATGTATGGACGCCTaat                                                     <  1:3694391/100‑3 (MQ=255)                                     aaTTTATAGTCTAATTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACTAATGCTGATTATGTATGGACGCCTATg                                                     <  1:4423326/100‑1 (MQ=255)                                          ataGTCTAATTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACTAATGCTGATTATGTATGGACGCCTATGACTAc                                                <  1:1115797/100‑1 (MQ=255)                                               cTAATTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACTAATGCTGATTATGTATGGACGCCTATGACTACAGTTa                                           >  1:3063417/1‑100 (MQ=255)                                                 acttGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACTAATGCTGATTATGTATGGACGCCTATGACTACAgatcgg                                         >  1:5192355/3‑95 (MQ=255)                                                 aaTTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACTAATGCTGATTATGTATGGACGCCTCTGACTACAGTTACg                                         >  1:4818575/1‑100 (MQ=255)                                                 aaTTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACTAATGCTGATTATGTATGGACGCCTATGACTACAGTTACg                                         >  1:3531668/1‑100 (MQ=255)                                                 aaTTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACTAATGCTGATTATGTATGGACGCCTATGACTACAGTTACg                                         >  1:1657665/1‑100 (MQ=255)                                                 aaTTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACTAATGCTGATTATGTATGGACGCCTATGACTACAGTTACg                                         >  1:3127661/1‑100 (MQ=255)                                                 aaTTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACTAATGCTGATTATGTATGGACGCCTATGACTACAGTTACg                                         >  1:853055/1‑100 (MQ=255)                                                 aaTTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACTAATGCTGATTATGTATGGACGCCTATGACTACAGTTACg                                         >  1:899336/1‑100 (MQ=255)                                                 aaTTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACTAATGCTGATTATGTATGGACGCCTATGACTACAGTTACg                                         >  1:4341455/1‑100 (MQ=255)                                                 aaTTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACTAATGCTGATTATGTATGGACGCCTATGACTACAGTTACg                                         >  1:3262936/1‑100 (MQ=255)                                                 aaTTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACTAATGCTGATTATGTATGGACGCCTATGACTACAGTTACg                                         >  1:3295935/1‑100 (MQ=255)                                                 aaTTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACTAATGCTGATTATGCATGGACGCCTATGACTACAGTTACg                                         >  1:3583100/1‑100 (MQ=255)                                                             cATCTGCATTATAAATTTTTTCATAATGTCCGCACCATTCTTAATGACTAATGCTGATTATGTATGGACGCCTATGACTACAGTTACGTTATTTATTTTg                             >  1:3094368/1‑100 (MQ=255)                                                              ctCTGCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACTAATGCTGATTATGTATGGACGCCTATGACTACAGTTACGTTATTTATTTTGa                            >  1:50456/2‑100 (MQ=255)                                                                cTGCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACTAATGCTGATTATGTATGGACGCCTATGACTACAGTTACGTTATTTATTTTTAGt                          >  1:5665857/1‑100 (MQ=255)                                                                cTGCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACTAATGCTGATTATGTATGGACGCCTATGACTACAGTTACGTTATTTATTTTGAGt                          >  1:2024450/1‑100 (MQ=255)                                                                  gCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACTAATGCTGATTATGTATGGACGCCTATGACTACAGTTACGTTATTTATTTTGAGttt                        >  1:20206/1‑100 (MQ=255)                                                                  gCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACTAATGCTGATTATGTATGGACGCCTATGACTACAGTTACGTTATTTATTTTGAGttt                        >  1:2473773/1‑100 (MQ=255)                                                                  gCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACTAATGCTGATTATGTATGGACGCCTATGACTACAGTTACGTTATTTATTTTGAGttt                        >  1:1300647/1‑100 (MQ=255)                                                                  gCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACTAATGCTGATTATGTATGGACGCCTATGACTACAGTTACGTTATTTATTTTGAGttt                        >  1:5784416/1‑100 (MQ=255)                                                                  gCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACTAATGCTGATTATGTATGGACGCCTATGACTACAGTTACGTTATTTATTTTGAGttt                        >  1:4659076/1‑100 (MQ=255)                                                                  gCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACTAATGCTGATTATGTATGGACGCCTATGACTACAGTTACGTTATTTATTTTGAGttt                        >  1:1695517/1‑100 (MQ=255)                                                                  gCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACTAATGCTGATTATGTATGGACGCCTATGACTACAGTTACGTTATTTATTTTGAGttt                        >  1:4155122/1‑100 (MQ=255)                                                                  gCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACTAATGCTGATTATGTATGGACGCCTATGACTACAGTTACGTTATTTATTTTGAGttt                        >  1:3257705/1‑100 (MQ=255)                                                                  gCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACTAATGCTGATTATGTATGGACGCCTATGACTACAGTTACGTTATTTATTTTGAGttt                        >  1:1668863/1‑100 (MQ=255)                                                                  gCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACTAATGCTGATTATGTATGGACGCCTATGACTACAGTTACGTTATTTATTTTGAGttg                        <  1:1592173/100‑2 (MQ=255)                                                                  gCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACTAATGCTGATTATGCATGGACGCCTATGACTACTGTTACGTTATTTATTTTGCGttt                        >  1:2864364/1‑100 (MQ=255)                                                                    aTTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACTAATGCTGATTATGTATGGACGCCTATGACTACAGTTACGTTATTTATTTTGAGTTTgc                      <  1:4121675/100‑2 (MQ=255)                                                                      tataAATTTTTTCATAATGGCCGCACCATTCCTAATGACTAATGCTGATTATGTATGGACGCCTATGACTACAGTTACGTTATTTATTTTGAGTTTGatt                    >  1:845953/1‑100 (MQ=255)                                                                        taAATTTTTTCATAATGGCCGCACCATTCTTAATGACTAATGCTGATTATGTATGGACGCCTATGACTACAGTTACGTTATTTATTTTGAGTTTGattat                  >  1:3249667/1‑100 (MQ=255)                                                                         aaaTTTTTTCATAATGGCCGCACCATTCTTAATGACTAATGCTGTTTATGTATGGACGCCTATGACTACTGTTACGTTATTTATTTTGAGTTTGcttatt                 >  1:919256/1‑100 (MQ=255)                                                                                   cccctGGCCGCACCATTCTTAATGACTAATGCTGATTATGTATGGACGCCTATGACTACAGTTACGTTATTTATTTTGAGTTTGATTATTTTCTTGatat       >  1:4317384/5‑100 (MQ=255)                                                                                   aTAATGGCCGCACCATTCTTAATGACTAATGCTGATTATGTATGGACGCCTATGACTACAGTTACGTTATTTATTTTGAGTTTGATTATTTTCTTGatat       >  1:586216/1‑100 (MQ=255)                                                                                    tAATGGCCGCACCATTCTTAATGACTACTGCTGATTATGTATGGACGCCTATGACTACAGTTACGTTATTTATTTTGAGTTTGATTATTTTCTTGATAtt      >  1:4543044/1‑100 (MQ=255)                                                                                    tAATGGCCGCACCATTCTTAATGACTAATGCTGATTATGTATGGACGCCTATGACTACAGTTACGTTATTTATTTTGAGTTTGATTATTTTCTTGATAtt      >  1:1583627/1‑100 (MQ=255)                                                                                     aaTGGCCGCACCATTCTTAATGACTAATGCTGATTATGTATGGACGCCTATGACTACAGTTACGTTATTTATTTTGAGTTTGATTATTTTCTTGATAttt     >  1:5314746/1‑100 (MQ=255)                                                                                     aaTGGCCGCACCATTCTTAATGACTAATGCTGATTATGTATGGACGCCTATGACTACAGTTACGTTATTTATTTTGAGTTTGATTATTTTCTTGATAttt     >  1:6603024/1‑100 (MQ=255)                                                                                     aaTGGCCGCACCATTCTTAATGACTAATGCTGATTATGTATGGACGCCTATGACTACAGTTACGTTATTTATTTTGAGTTTGATTATTTTCTTGATAttt     >  1:1806448/1‑100 (MQ=255)                                                                                     aaTGGCCGCACCATTCTTAATGACTAATGCTGATTATGTATGGACGCCTATGACTACAGTTACGTTATTTATTTTGAGTTTGATTATTTTCTTGATAttt     >  1:2524062/1‑100 (MQ=255)                                                                                     aaTGGCCGCACCATTCTTAATGACTAATGCTGATTATGTATGGACGCCTATGACTACAGTTACGTTATTTATTTTGAGTTTGATTATTTTCTTGATAttt     >  1:3310844/1‑100 (MQ=255)                                                                                      ttGGCCGCACCATTCTTAATGACTAATGCTGATTATGTATGGACGCCTATGACTACAGTTACGTTATTTATTTTGAGTTTGATTATTTTCTTGATATTta    >  1:6025043/2‑100 (MQ=255)                                                                                      aTGGCCGCACCATTCTTAATGACTAATGCTGATTATGTATGGACGCCTATGACTACAGTTACGTTATTTATTTTGAGTTTGATTATTTTCTTGATATTta    >  1:5609888/1‑100 (MQ=255)                                                                                      aTGGCCGCACCATTCTTAATGACTAATGCTGATTATGTATGGACGCCTATGACTACAGTTACGTTATTTATTTTGAGTTTGATTATTTTCTTGATATTta    >  1:4739590/1‑100 (MQ=255)                                                                                      aTGGCCGCACCATTCTTAATGACTAATGCTGATTATGTATGGACGCCTATGACTACAGTTACGTTATTTATTTTGAGTTTGATTATTTTCTTGATATTta    >  1:1102169/1‑100 (MQ=255)                                                                                        ggCCGCACCATTCTTAATGACTAATTCTGATTTTGTATGGACGCCTATGACTACAGTTACGTTATTTATTTTGAGTTTGATTATTTTCTTGATATTtata  >  1:2805839/1‑100 (MQ=255)                                                                                        ggCCGCACCATTCTTAATGACTAATGCTGATTATGTATGGACGCCTATGACTACAGTTACGTTATTTATTTTGAGTTTGATTATTTTCTTGatatagatc  >  1:3205050/1‑95 (MQ=255)                                                                                        ggCCGCACCATTCTTAATGACTAATGCTGATTATGTATGGACGCCTATGACTACAGTTACGTTATTTATTTTGAGTTTGATTATTTTCTTGATATTtata  >  1:255113/1‑100 (MQ=255)                                                                                        ggCCGCACCATTCTTAATGACTAATGCTGATTATGTATGGACGCCTATGACTACAGTTACGTTATTTATTTTGAGTTTGATTATTTTCTTGATATTtata  >  1:3215246/1‑100 (MQ=255)                                                                                           |                                                                                                 TATTTATTTATGAAGGGTGTGTATTGTTATTTATCTAATTTATAGTCTAATTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCTGCACCATTCTTAATGACTAATGCTGATTATGTATGGACGCCTATGACTACAGTTACGTTATTTATTTTGAGTTTGATTATTTTCTTGATATTTATA  >  NC_007793/2085532‑2085718

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑