Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,085,565	T→C	intergenic (‑93/‑52)	SAUSA300_RS10515 ← / → SAUSA300_RS10520	hypothetical protein/membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,085,565	0	T	C	100.0%	118.0 / NA	45	intergenic (‑93/‑52)	SAUSA300_RS10515/SAUSA300_RS10520	hypothetical protein/membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (39/6);  total (39/6)

ACCAATATTTTTTATTTTCTACACTTCTGTGTGTTACTTTTGTTAAAATATATAGGATTTAAATTATTTATTTATGAAGGGTGTGTATTGTTATTTATCTAATTTATAGTCTAATTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCTGCACCATTCTTAATGACTAATGCTGATTATGTATGGAC  >  NC_007793/2085468‑2085660                                                                                                  |                                                                                                aCCAATATTTTTTATTTTCTACACTTCTGTGTTTTACTTTTGTTAAAATATATAGGATTTAAATTATTTATTTATGAAGGGTGTGTATTGTTATTTACCt                                                                                               <  1:1859248/100‑1 (MQ=255)  ccAATATTTTTTATTTTCTACACTTCTGTGTTTTACTTTTGTTAAAATATATAGGATTTAAATTATTTATTTATGAAGGGTGTGTATTGTTATTTACCTa                                                                                              >  1:4460145/1‑100 (MQ=255)         tttttATTTTCTACACTTCTGTGTTTTGCTTTTGTTAAAATATATAGGATTTAAATTATTTATTTATGAAGGGTGTGTATTGTTATTTACCTAATTtagg                                                                                       <  1:474212/100‑3 (MQ=255)                  tCTACACTTCTGTGTTTTACTTTTGTTAAAATATATAGGATTTAAATTATTTATTTATGAAGGGTGTGTATTGTTATTTACCTAATTTATAGTCTAATTg                                                                              >  1:624076/1‑100 (MQ=255)                     acacTTCTGTGTTTTACTTTTGTTAAAATATATAGGATTTAAATTATTTATTTATGAAGGGTGTGTATTGTTATTTACCTAATTTATAGTCTAATTGTCt                                                                           <  1:5328944/100‑1 (MQ=255)                     acacTTCTGTGTTTTACTTTTGTTAAAATATATAGGATTTAAATTATTTATTTATGAAGGGTGTGTATTGTTATTTACCTAATTTATAGTCTAATTGTCt                                                                           >  1:5078324/1‑100 (MQ=255)                     acacTTCTGTGTTTTACTTTTGTTAAAATATATAGGATTTAAATTATTTATTTATGAAGGGTGTGTATTGTTATTTACCTAATTTATAGTCTAATTGTCt                                                                           >  1:6040196/1‑100 (MQ=255)                          tCTGTGTTTTACTTTTGTTAAAATATATAGGATTTAAATTATTTATTTATGAAGGGTGTGTATTGTTATTTACCTAATTTATAGTCTAATTGTCTATTTc                                                                      >  1:644462/1‑100 (MQ=255)                             gtgtttTACTTTTGTTAAAATATATAGGATTTAAATTATTTATTTATGAAGGGTGTGTATTGTTATTTACCTAATTTATAGTCTAATTGTCTATTTCATc                                                                   >  1:1265840/1‑100 (MQ=255)                                  ttACTTTTGTTAAAATATATAGGATTTAAATTATTTATTTATGAAGGGTGTGTATTGTTATTTACCTAATTTATAGTCTAATTGTCTATTTCATCTGCAt                                                              >  1:6600549/1‑100 (MQ=255)                                   tACTTTTGTTAAAATATATAGGATTTAAATTATTTATTTATGAAGGGTGTGTATTGTTATTTTCCTAATTTATAGTCTAATTGTCTATTTCATCTGCAtt                                                             <  1:6077943/100‑1 (MQ=255)                                      ttttGTTAAAATATATAGGATTTAAATTATTTATTTATGAAGGGTGTGTATTGTTATTTACCTAATTTATAGTCTAATTGTCTATTTCATCTGCATtata                                                          <  1:2215473/100‑1 (MQ=255)                                                  atataGGATTTAAATTATTTATTTATGAAGGGTGTGTATTGTTATTTACCTAATTTATAGTCTAATTGTCTATTTCATCTGCATTATAAATTTTTTCATa                                              >  1:3159945/1‑100 (MQ=255)                                                  atataGGATTTAAATTATTTATTTATGAAGGGTGTGTATTGTTATTTACCTAATTTATAGTCTAATTGTCTATTTCATCTGCATTATAAATTTTTTCATa                                              >  1:869391/1‑100 (MQ=255)                                                  atataGGATTTAAATTATTTATTTATGAAGGGTGTGTATTGTTATTTACCTAATTTATAGTCTAATTGTCTATTTCATCTGCATTATAAATTTTTTCATa                                              >  1:5079437/1‑100 (MQ=255)                                                                 aatttatttatGAAGGGTGTGTATTGTTATTTACCTAATTTATAGTCTAATTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCCGCACCATTc                               >  1:5163377/2‑100 (MQ=255)                                                                          tcttaGGGTGTGTATTGTTATTTACCTAATTTATAGTCTAATTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACt                      >  1:6371741/5‑100 (MQ=255)                                                                          gtGAAGGGTGTGTATTGTTATTTACCTAATTTATAGTCTAATTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACt                      >  1:6441308/2‑100 (MQ=255)                                                                          atGAAGGGTGTGTATTGTTATTTACCTAATTTATAGTCTAATTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACt                      >  1:5582369/1‑100 (MQ=255)                                                                          atGAAGGGTGTGTATTGTTATTTACCTAATTTATAGTCTAATTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACt                      >  1:1765905/1‑100 (MQ=255)                                                                          atGAAGGGTGTGTATTGTTATTTACCTAATTTATAGTCTAATTGTCTATTTCATCTGCATCATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACt                      >  1:5940493/1‑100 (MQ=255)                                                                           tGAAGGGTGTGTATTGTTATTTACCTAATTTATAGTCTAATTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACTa                     >  1:5666306/1‑100 (MQ=255)                                                                           tGAAGGGTGTGTATTGTTATTTACCTAATTTATAGTCTAATTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACTa                     >  1:368058/1‑100 (MQ=255)                                                                           tGAAGGGTGTGTATTGTTATTTACCTAATTTATAGTCTAATTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACTa                     >  1:1524875/1‑100 (MQ=255)                                                                            tcaGGGTGTGTATTGTTATTTACCTAATTTATAGTCTAATTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACTaa                    >  1:4360632/3‑100 (MQ=255)                                                                            gAAGGGTGTGTATTGTTATTTACCTAATTTATAGTCTAATTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACTaa                    >  1:558133/1‑100 (MQ=255)                                                                            gAAGGGTGTGTATTGTTATTTACCTAATTTATAGTCTAATTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACTaa                    >  1:4756706/1‑100 (MQ=255)                                                                            ccaGGGTGTGTATTGTTATTTACCTAATTTATAGTCTAATTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACTaa                    >  1:4163040/3‑100 (MQ=255)                                                                             gaGGGTGTGTATTGTTATTTACCTAATTTATAGTCTAATTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACTAAt                   >  1:4207691/2‑100 (MQ=255)                                                                             gaGGGTGTGTATTGTTATTTACCTAATTTATAGTCTAATTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACTAAt                   >  1:5463485/2‑100 (MQ=255)                                                                               gggTGTGTATTGTTATTTACCTAATTTATAGTCTAATTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCCGCACGATTCTTAATGACTaagtt                 <  1:4073154/100‑4 (MQ=255)                                                                                ggTGTGTATTGTTATTTACCTAATTTATAGTCTAATTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACTAATGCt                >  1:965503/1‑100 (MQ=255)                                                                                 ctgtgtATTGTTATTTACCTAATTTATAGTCTAATTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACTAATGCTg               >  1:2141846/2‑100 (MQ=255)                                                                                  tgtgtATTGTTATTTACCTAATTTATAGTCTAATTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCCGCACCATTCTTGATGACTAATGCTGa              >  1:4982191/1‑100 (MQ=255)                                                                                   gtgtATTGTTATTTACCTAATTTATAGTCTAATTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCCGCACCATTCTTACTGACTAATGCTGAt             >  1:4974925/1‑100 (MQ=255)                                                                                   gtgtATTGTTATTTACCTAATTTATAGTCTAATTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACTAATGCTGAt             >  1:4709487/1‑100 (MQ=255)                                                                                   gtgtATTGTTATTTACCTAATTTATAGTCTAATTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACTAATGCTGAt             >  1:6364453/1‑100 (MQ=255)                                                                                   gtgtATTGTTATTTACCTAATTTATAGTCTAATTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACTAATGCTGAt             >  1:3633564/1‑100 (MQ=255)                                                                                   gtgtATTGTTATTTACCTAATTTATAGTCTAATTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACTAATGCTGAt             >  1:35642/1‑100 (MQ=255)                                                                                   gtgtATTGTTATTTACCTAATTTATAGTCTAATTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACTAATGCTGAt             >  1:3090003/1‑100 (MQ=255)                                                                                   gtgtATTGTTATTTACCTAATTTATAGTCTAATTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACTAATGCTGAt             >  1:160310/1‑100 (MQ=255)                                                                                   gtgtATTGTTATTTACCTAATTTATAGTCTAATTGTCTATCTCATCTGCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACTAATGCTGAt             >  1:4081710/1‑100 (MQ=255)                                                                                       aTTGTTATTTACCTAATTTATAGTCTAATTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACTAATGCTGATtatg         >  1:5573341/1‑100 (MQ=255)                                                                                       aTTGTTATTTACCTAATTTATAGTCTAATTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACTAATGCTGATtatg         >  1:5444619/1‑100 (MQ=255)                                                                                         tGTTATTTACCTAATTTATAGTCTAATTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACTAATGCTGATtatgta       >  1:4066915/1‑100 (MQ=255)                                                                                            tatttacCTAATTTATAGTCTAATTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACTAATGCTGATTATGTATgg    <  1:646319/100‑1 (MQ=255)                                                                                              cttacCTAATTTATAGTCTAATTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCCGCACCATTCTTAATGACTAATGCTGATTATGTATGGAc  >  1:5919566/2‑100 (MQ=255)                                                                                                  |                                                                                                ACCAATATTTTTTATTTTCTACACTTCTGTGTGTTACTTTTGTTAAAATATATAGGATTTAAATTATTTATTTATGAAGGGTGTGTATTGTTATTTATCTAATTTATAGTCTAATTGTCTATTTCATCTGCATTATAAATTTTTTCATAATGGCTGCACCATTCTTAATGACTAATGCTGATTATGTATGGAC  >  NC_007793/2085468‑2085660

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑