Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,085,398	A→G	I25I (ATT→ATC)	SAUSA300_RS10515 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,085,398	0	A	G	99.0%	261.1 / ‑5.6	96	I25I (ATT→ATC)	SAUSA300_RS10515	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (92/3);  total (93/3)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.65e-01

GTCAACTCATTAACTCATTTTCTAACTCTAAACACCTCATTTTTTAATAATTCAGCATCGGATTTCTGTTTTATCATAGGGGCTATATTTTTCTTA‑ATCGGAATTTTTGTTGCAATATACGGTATGAAGCGAGCAACATATTGGTTAAACTTATTGATTTTATTTACCAATATTTTT  >  NC_007793/2085303‑2085479                                                                                                |                                                                                   accAACTCATTAACTCATTTTCTAACTCTAAACACCTCATTTTTTAATAATTCAGCATCGGATTTCTGTTTTATCATAGGGGCTATATTTTTCTTG‑ATCg                                                                               >  1:3248453/3‑100 (MQ=255)   cAACTCATTAACTCATTTTCTAACTCTAAACACCTCATTTTTTAATAATTCAGCATCGGATTTCTGTTTTATCATAGGGGCTATATTTTTCCTG‑ATCGGa                                                                             >  1:2423267/1‑100 (MQ=255)        cattaactcattTTCTAACTCTAAACACCTCATTTTTTAATAATTCAGCATCGGATTTCTGTTTTATCATAGGGGCTATATTTTTCTTG‑ATCGGAAtttt                                                                        >  1:3490473/1‑100 (MQ=255)        cattaactcattTTCTAACTCTAAACACCTCATTTTTTAATAATTCAGCATCGGATTTCTGTTTTATCATAGGGGCTATATTTTTCTTG‑ATCGGAAtttt                                                                        >  1:2113856/1‑100 (MQ=255)        cattaactcattTTCTAACTCTAAACACCTCATTTTTTAATAATTCAGCATCGGATTTCTGTTTTATCATAGGGGCTATATTTTTCTTG‑ATCGGAAtttt                                                                        >  1:6239426/1‑100 (MQ=255)           taactcattTTCTAACTCTAAACACCTCATTTTTTAATAATTCAGCATCGGATTTCTGTTTTATCATAGGGGCTATATTTTTCTTG‑ATCGGAATTTttgt                                                                     >  1:60393/1‑100 (MQ=255)           taactcattTTCTAACTCTAAACACCTCATTTTTTAATAATTCAGCATCGGATTTCTGTTTTATCATAGGGGCTATATTTTTCTAG‑ATCGGaagagcaca                                                                     >  1:254728/1‑93 (MQ=255)            gactcattTTCTAACTCTAAACACCTCATTTTTTAATAATTCAGCATCGGATTTCTGTTTTATCATAGGGGCTATATTTTTCTTG‑ATCGGAATTTttgtt                                                                    >  1:3540037/2‑100 (MQ=255)            aactcattTTCTAACTCTAAACACCTCATTTTTTAATAATTCAGCATCGGATTTCTGTTTTATCATAGGGGCTATATTTTTCTTG‑ATCGGAATTTttgtt                                                                    >  1:298754/1‑100 (MQ=255)            aactcattTTCTAACTCTAAACACCTCATTTTTTAATAATTCAGCATCGGATTTCTGTTTTATCATAGGGGCTATATTTTTCTTG‑ATCGGAATTTttgtt                                                                    >  1:4481755/1‑100 (MQ=255)            aactcattTTCTAACTCTAAACACCTCATTTTTTAATAATTCAGCATCGGATTTCTGTTTTATCATAGGGGCTATATTTTTCTTG‑ATCGGAATTTtggtt                                                                    >  1:4577360/1‑100 (MQ=255)            aactcattTTCTAACTCTAAACACCTCATTTTTTAATAATTCAGCATCGGATTTCTGTTTTATCATAGGGGCTATATTCTTCTAG‑ATCGGaagagcacac                                                                    >  1:3155680/1‑92 (MQ=255)            aactcattTTCTAACTCTAAACACCTCATTTTTTAATAATTCAGCATCGGATTTCTGTTTTACCATAGGGGCTATATTTTTCTTG‑ATCGGAATTTttgtt                                                                    >  1:5867963/1‑100 (MQ=255)            aactcattTTCTAACTCTAAACACCTCATTTTTTAATAATTCACCTCCGGTATTCCGTTTTATCATAGGGGCTATATTTTTCTTG‑ATCGGAATTTttgct                                                                    >  1:5913965/1‑98 (MQ=255)            aactcattTTCTAACTCCAAACACCTCATTTTTTAATAATTCAGCATCGGATTTCTGTTTTATCATAGGGGCTATATTTTTCTTG‑ATCGGAATTTttgtt                                                                    >  1:6530929/1‑100 (MQ=255)             actcattTTCTAACTCTAAACACCTCATTTTTTAATAATTCAGCATCGGATTTCTGTTTTATCATAGGGGCTATATTTTTCTCAGATCGGaagagcacac                                                                    >  1:3520635/1‑92 (MQ=255)               tcattTTCTAACTCTAAACACCTCATTTTTTAATAATTCAGCATCGGATTTCTGTTTTATCATAGGGGCTATATTTTTCTTG‑ATCGGAATTTTTGTTGCa                                                                 <  1:2660535/100‑1 (MQ=255)                cattTTCTAACTCTAAACACCTCATTTTTTAATAATTCAGCATCGGATTTCTGTTTTATCATATGGGCTATATTTTTCTTG‑ATCGGAATTTttgtaacga                                                                >  1:4664167/1‑95 (MQ=255)                cattTTCTAACTCTAAACACCTCATTTTTTAATAATTCAGCATCGGATTTCTGTTTTATCATAGGGGCTATATTTTTCTTG‑ATCGGAATTTTTGTTGCaa                                                                >  1:789987/1‑100 (MQ=255)                cattTTCTAACTCTAAACACCTCATTTTTTAATAATTCAGCATCGGATTTCTGTTTTATCATAGGGCCTATATTTTTCTTG‑ATCGGAATTTTTGTTGCaa                                                                >  1:3899434/1‑100 (MQ=255)                     tCTAACTCTAAACACCTCATTTTTTAATAATTCAGCATCGGATTTCTGTTTTATCATAGGGGCTATATTTTTCTTG‑ATCGGAATTTTTGTTGCAATATAc                                                           >  1:2960104/1‑100 (MQ=255)                      cTAACTCTAAACACCTCATTTTTTAATAATTCAGCATCGGATTTCTGTTTTATCATAGGGGCTATATTTTTCTTG‑ATCGGAATTTTTGTCGCAagatcgg                                                          >  1:5149036/1‑97 (MQ=255)                        aaCTCTAAACACCTCATTTTTTAATAATTCAGCATCGGATTTCTGTTTTATCATAGGGGCTATATTTTTCTTG‑ATCGGAATTTTTGTTGCaacagatcgg                                                        >  1:606477/1‑92 (MQ=255)                        aaCTCTAAACACCTCATTTTTTAATAATTCAGCATCGGATTTCTGTTTTATCATAGGGGCTATATTTTTCTTG‑ATCGGAATTTTTGTTGCaaaagatcgg                                                        >  1:924511/1‑92 (MQ=255)                        aaCTCTAAACACCTCATTTTTTAATAATTCAGCATCGGATTTCTGTTTTATCATAGGGGCTATATTTTTCTTG‑ATCGGAATTTTTGTTGCAatacagatc                                                        >  1:5347099/1‑94 (MQ=255)                        aaCTCTAAACACCTCATTTTTTAATAATTCAGCATCGGATTTCTGTTTTATCATAGGGGCTATATTTTTCTTG‑ATCGGAATTTTTGTTGCAATATACgga                                                        >  1:1961364/1‑99 (MQ=255)                        aaCTCTAAACACCTCATTTTTTAATAATTCAGCATCGGATTTCTGTTTTATCATAGGGGCTATATTTTTCTTG‑ATCGGAATTTTTGTTGCAATATACgct                                                        >  1:6176051/1‑98 (MQ=255)                        aaCTCTAAACACCTCATTTTTTAATAATTCAGCATCGGATTTCTGTTTTATCATAGGGGCTATATTTTTCTTG‑ATCGGAATTTTTGTTGCAATATACGGt                                                        >  1:3352822/1‑100 (MQ=255)                        aaCTCTAAACACCTCATTTTTTAATAATTCAGCATCGGATTTCTGTTTTATCATAGGGGCTATATTTTTCTTG‑ATCGGAATTTTTGTTGCAATATACGGt                                                        >  1:1036284/1‑100 (MQ=255)                        aaCTCTAAACACCTCATTTTTTAATAATTCAGCATCGGATTTCTGTTTTATCATAGGGGCTATATTTTTCTTG‑ATCGGAATTTTTGTTGCAATATACGGt                                                        >  1:1811367/1‑100 (MQ=255)                        aaCTCTAAACACCTCATTTTTTAATAATTCAGCATCGGATTTCTGTTTTATCATAGGGGCTATATTTTTCTTG‑ATCGGAATTTTTGTTGCAATATACGGt                                                        >  1:6101711/1‑100 (MQ=255)                        aaCTCTAAACACCTCATTTTTTAATAATTCAGCATCGGATTTCTGTTTTATCATAGGGGCTATATTTTTCTTG‑ATCGGAATTTTTGTTGCAATATACGGt                                                        >  1:1480079/1‑100 (MQ=255)                        aaCTCTAAACACCTCATTTTTTAATAATTCAGCATCGGATTTCTGTTTTATCATAGGGGCTATATTTTTCTTG‑ATCGGAATTTTTGTTGCAATATACGGt                                                        >  1:1783838/1‑100 (MQ=255)                        aaCTCTAAACACCTCATTTTTTAATAATTCAGCATCGGATTTCTGTTTTATCATAGGGGCTATATTTTTCTTG‑ATCGGAATTTTTGTTGCAATACACGGt                                                        >  1:979180/1‑100 (MQ=255)                        aaCTCTAAACACCTCATTTTTTAATAATTCAGCATCGGATTTCTGTTTTATCATAGGGGCTATATTTTTCTTG‑ATCGGAATTTTTGTTGCAACATACgag                                                        >  1:2921502/1‑98 (MQ=255)                        aaCTCTAAACACCTCATTTTTTAATAATTCAGCATCGGATTTCTGTTTTATCATAGGGGCTATATTTTTCTTG‑ATCGGAATTTTTGTTGCAACATACgag                                                        >  1:5376091/1‑98 (MQ=255)                        aaCTCTAAACACCTCATTTTTTAATAATTCAGCATCGGATTTCTGTTTTATCATAGGGGCTATATTTTTCTTG‑ATCGGAATTTTTGTTACAATATACGGt                                                        >  1:5525992/1‑100 (MQ=255)                        aaCTCTAAACACCTCATTTTTTAATAATTCAGCATCGGATTTCTGCTTTATCATAGGGGCTATATTTTTCTTG‑ATCGGAATTTTTGTTGCAATATACgct                                                        >  1:5614293/1‑98 (MQ=255)                        aaCTCTAAACACCTCATTTTTTAATAATTCAGCATCGGATCTCTGTTTTATCATAGGGGCTATATTTTTCTTG‑ATCGGAATTTTTGTTGCAATATACGGt                                                        >  1:689843/1‑100 (MQ=255)                            ctAAACACCTCATTTTTTAATAATTCAGCATCGGATTTCTGTTTTATCATATGGGCTATATTTTTCTTG‑ATCAGAATTTTTGCTGCAagatcggaagagc                                                    >  1:4877168/1‑91 (MQ=255)                            ctAAACACCTCATTTTTTAATAATTCAGCATCGGATTTCTGTTTTATCATAGGGGCTATATTTTTCTTG‑ATCGGAATTTTTGTTGCAATATACggcagat                                                    >  1:2079123/1‑95 (MQ=255)                            ctAAACACCTCATTTTTTAATAATTCAGCATCGGATTTCTGTTTTATCATAGGGGCTATATTTTTCTTG‑ATCGGAATTTTTGTTGCAATATACGGTAAGa                                                    >  1:1909271/1‑100 (MQ=255)                              acacacCTCATTTTTTAATAATTCAGCATCGGCTTTCTGTTTTATCATAGGGGCTATCTTTTTCTTG‑ATCGGAATTTTTGTTGCATTATACGGTagatcg                                                  >  1:1106246/3‑95 (MQ=255)                              aaaCACCTCATTTTTTAATACTTCAGCATCGGATTTCTGTTTTATCATAGGGGCTATATTTTTCTTG‑ATCGGAATTTTTGTTGCAATATACggccagatc                                                  >  1:2264801/1‑93 (MQ=255)                              aaaCACCTCATTTTTTAATAATTCAGCATCGGATTTCTGTTTTATCATAGGGGCTATATTTTTCTTG‑ATCGGAATTTTTGTTGCGAtataagatcggaag                                                  >  1:6506459/1‑90 (MQ=255)                              aaaCACCTCATTTTTTAATAATTCAGCATCGGATTTCTGTTTTATCATAGGGGCTATATTTTTCTTG‑ATCGGAATTTTTGTTGCAGTATACGGtcagatc                                                  >  1:4931197/1‑94 (MQ=255)                              aaaCACCTCATTTTTTAATAATTCAGCATCGGATTTCTGTTTTATCATAGGGGCTATATTTTTCTTG‑ATCGGAATTTTTGTTGCAATGTACggagatcgg                                                  >  1:1435102/1‑93 (MQ=255)                              aaaCACCTCATTTTTTAATAATTCAGCATCGGATTTCTGTTTTATCATAGGGGCTATATTTTTCTTG‑ATCGGAATTTTTGTTGCAATCTACGGGAtagat                                                  >  1:6041202/1‑96 (MQ=255)                              aaaCACCTCATTTTTTAATAATTCAGCATCGGATTTCTGTTTTATCATAGGGGCTATATTTTTCTTG‑ATCGGAATTTTTGTTGCAATATAccagatcgga                                                  >  1:556246/1‑91 (MQ=255)                              aaaCACCTCATTTTTTAATAATTCAGCATCGGATTTCTGTTTTATCATAGGGGCTATATTTTTCTTG‑ATCGGAATTTTTGTTGCAATATACgggagatcg                                                  >  1:6596740/1‑93 (MQ=255)                              aaaCACCTCATTTTTTAATAATTCAGCATCGGATTTCTGTTTTATCATAGGGGCTATATTTTTCTTG‑ATCGGAATTTTTGTTGCAATATACggctgagat                                                  >  1:3066762/1‑93 (MQ=255)                              aaaCACCTCATTTTTTAATAATTCAGCATCGGATTTCTGTTTTATCATAGGGGCTATATTTTTCTTG‑ATCGGAATTTTTGTTGCAATATACggcagatcg                                                  >  1:1433471/1‑93 (MQ=255)                              aaaCACCTCATTTTTTAATAATTCAGCATCGGATTTCTGTTTTATCATAGGGGCTATATTTTTCTTG‑ATCGGAATTTTTGTTGCAATATACggcagatcg                                                  >  1:1885499/1‑93 (MQ=255)                              aaaCACCTCATTTTTTAATAATTCAGCATCGGATTTCTGTTTTATCATAGGGGCTATATTTTTCTTG‑ATCGGAATTTTTGTTGCAATATACggcagatcg                                                  >  1:3992076/1‑93 (MQ=255)                              aaaCACCTCATTTTTTAATAATTCAGCATCGGATTTCTGTTTTATCATAGGGGCTATATTTTTCTTG‑ATCGGAATTTTTGTTGCAATATACggactagat                                                  >  1:6194616/1‑93 (MQ=255)                              aaaCACCTCATTTTTTAATAATTCAGCATCGGATTTCTGTTTTATCATAGGGGCTATATTTTTCTTG‑ATCGGAATTTTTGTTGCAATATACggaaagatc                                                  >  1:2996665/1‑93 (MQ=255)                              aaaCACCTCATTTTTTAATAATTCAGCATCGGATTTCTGTTTTATCATAGGGGCTATATTTTTCTTG‑ATCGGAATTTTTGTTGCAATATACGGTagatcg                                                  >  1:3009217/1‑95 (MQ=255)                              aaaCACCTCATTTTTTAATAATTCAGCATCGGATTTCTGTTTTATCATAGGGGCTATATTTTTCTTG‑ATCGGAATTTTTGTTGCAATATACGGTagagat                                                  >  1:2649593/1‑95 (MQ=255)                              aaaCACCTCATTTTTTAATAATTCAGCATCGGATTTCTGTTTTATCATAGGGGCTATATTTTTCTTG‑ATCGGAATTTTTGTTGCAATATACGGTacagat                                                  >  1:3607563/1‑95 (MQ=255)                              aaaCACCTCATTTTTTAATAATTCAGCATCGGATTTCTGTTTTATCATAGGGGCTATATTTTTCTTG‑ATCGGAATTTTTGTTGCAATATACGGTaaagat                                                  >  1:2871992/1‑95 (MQ=255)                              aaaCACCTCATTTTTTAATAATTCAGCATCGGATTTCTGTTTTATCATAGGGGCTATATTTTTCTTG‑ATCGGAATTTTTGTTGCAATATACGGTAtagat                                                  >  1:226569/1‑96 (MQ=255)                              aaaCACCTCATTTTTTAATAATTCAGCATCGGATTTCTGTTTTATCATAGGGGCTATATTTTTCTTG‑ATCGGAATTTTTGTTGCAATATACGGTATGAAg                                                  >  1:3016129/1‑100 (MQ=255)                              aaaCACCTCATTTTTTAATAATTCAGCATCGGATTTCTGTTTTATCATAGGGGCTATATTTTTCTTG‑ATCGGAATTTTTGTTGCAATATACGGTATGAAg                                                  >  1:2545550/1‑100 (MQ=255)                              aaaCACCTCATTTTTTAATAATTCAGCATCGGATTTCTGTTTTATCATAGGGGCTATATTTTTCTTG‑ATCGGAATTTTTGTTGCAATATACGGTAAGatc                                                  >  1:5009638/1‑98 (MQ=255)                              aaaCACCTCATTTTTTAATAATTCAGCATCGGATTTCTGTTTTATCATAGGGGCTATATTTTTCTTG‑ATCGGAATTTTTGTTGCAATATACGGGAtagat                                                  >  1:3834329/1‑96 (MQ=255)                              aaaCACCTCATTTTTTAATAATTCAGCATCGGATTTCTGTTTTATCATAGGGGCTATATTTTTCTTG‑ATCGGAATTTTTGTTGCAATAAACggcagatcg                                                  >  1:6623307/1‑93 (MQ=255)                              aaaCACCTCATTTTTTAATAATTCAGCATCGGATTTCTGTTTTATCATAGGGGCTATATTTTTCTTG‑ATCGGAA‑TTTTGTTGCAATATACGGTagatcgg                                                 >  1:6216894/1‑94 (MQ=255)                              aaaCACCTCATTTTTTAATAATTCAGCATCGGATTTCTGTTTTATCATAGGGGCTATATTTTTCTGG‑ATCGGAATTTTTGTTGCAATATACggcaagatc                                                  >  1:4306685/1‑93 (MQ=255)                              aaaCACCTCATTTTTTAATAATTCAGCAACGGATTTCTGTTTTATCATATTGGCTATCTTTTTCTTG‑TTCGGACTTTTTCTTGCAATATACGGTATGAAg                                                  >  1:6302152/1‑100 (MQ=255)                               cacacCTCATTTTTTAATAATTCAGCATCGGATTTCTGTTTTATCATAGGGGCTATATTTTTCTTG‑ATCGGAATTTTTGTTGCAATACACGGTagatcgg                                                 >  1:6049054/2‑94 (MQ=255)                                acacCTCATTTTTTAATAATTCAGCATCGGATTTCTGTTTTATCATAGGGGCTATATTTTTCTTG‑ATCGGAATTTTTGTTGCAATATACGGTATGAAGCg                                                <  1:6327130/100‑1 (MQ=255)                                 tcccTCATTTTTTAATAATTCAGCATCGGGTTTCTGTTTTAGCATAGGGGCTATATTTTTCTTG‑ATCGGAATTTTTGTTGCAATATACGGTATGAAgatc                                               >  1:1153377/3‑97 (MQ=255)                                  acCTCATTTTTTAATAATTCAGCATCGGATTTCTGTTTTATCATAGGGGCTATATTTTTCTTG‑ATCGGAATTTTTGTTGCAATATCCGGtgcagatcgga                                              >  1:3823166/1‑90 (MQ=255)                                  acCTCATTTTTTAATAATTCAGCATCGGATTTCTGTTTTATCATAGGGGCTATATTTTTCTTG‑ATCGGAATTTTTGTTGCAATACACGGTagatcggaag                                              >  1:5458390/1‑91 (MQ=255)                                  acCTCATTTTTTAATAATTCAGCATCGGATTTCTGTTTTACCATAGGGGCTATATTTTTCTTG‑ATCGGAATTTTTGTTGCAATATACGGtgtagatcgga                                              >  1:6000110/1‑90 (MQ=255)                                   ccTCATTTTTTAATAATTCAGCATCGGATTTCTGTTTTATCATCGGGGCTATATTCTTCTTG‑ATCGGAATTTTTGTTGCAATATACGGGAtagatcggaa                                             >  1:3774573/1‑91 (MQ=255)                                   ccTCATTTTTTAATAATTCAGCATCGGATTTCTGTTTTATCATAGGGGCTATATTTTTCTTG‑ATCGGAATTTTTGTTGCAATATACGGTATGagatcgga                                             >  1:456384/1‑93 (MQ=255)                                   ccTCATTTTTTAATAATTCAGCATCGGATTTCTGTTTTATCATAGGGGCTATATTTTTCTTG‑ATCGGAATTTTTGTTGCAATATACGGTAAGatcggaag                                             >  1:3124590/1‑93 (MQ=255)                                     tCATTTTTTAATAATTCAGCATCGGATTTCTGTTTTATCATAGGGGCTATATTTTTCTTG‑ATCGGAATTTTTGTTGCAATATACGGTAAGatcggaagag                                           >  1:5187683/1‑91 (MQ=255)                                             taataaTTCAGCATCGGATTTCTGTTTTATCATAGGGGCTATATTTTTCTTG‑ATCGGAATTTTTGTTGCAATATACGGTATGAAGCGAGCAACATATTgg                                   <  1:3099266/100‑1 (MQ=255)                                                    tCAGCATCGGATTTCTGTTTTATCATAGGGGCTATATTTTTCTTG‑ATCGGAATTTTTGTTGCAATATACGGTATGAAGCGAGCAACATATTGGTTAAACt                            >  1:3286221/1‑100 (MQ=255)                                                     cAGCATCGGATTTCTGTTTTATCATAGGGGCTATATTTTTCTTG‑ATCGGAATTTTTGTTGCAATATACGGTATGAAGCGAGCAACATATTGGTTAAACtt                           >  1:4493289/1‑100 (MQ=255)                                                           cGGATTTCTGTTTTATCATAGGGGCTATATTTTTCTTG‑ATCGGAATTTTTGTTGCAATATACGGTATGAAGCGAGCAACATATTGGTTAAACTTATTGAt                     >  1:4087877/1‑100 (MQ=255)                                                              ctttCTGTTTTATCATAGGGGCTATATTTTTCTTG‑ATCGGAATTTTTGTTGCAATATACGGTATGAAGCGAGCAACATATTGGTTAAACTTATTGAtttt                  >  1:3786506/2‑100 (MQ=255)                                                                        tATCATAGGGGCTATATTTTTCTTG‑ATCGGAATTTTTGTTGCAATATACGGTATGAAGCGAGCAACATATTGGTTAAACTTATTGATTTTATTTACCAat        >  1:2154055/1‑100 (MQ=255)                                                                          tCATAGGGGCTATATTTTTCTTG‑ATCGGAATTTTTGTTGCAATATACGGTATGAAGCGAGCAACATATTGGTTAAACTTATTGATTTTATTTACCAatat      >  1:6515642/1‑100 (MQ=255)                                                                           cATAGGGGCTATATTTTTCTTG‑ATCGGAATTTTTGTTGCACTATACGGTATGAAGCGAGCAACATATTGGTTAAACTTATTGATTTTATTTACCAATAtt     >  1:1696048/1‑100 (MQ=255)                                                                           cATAGGGGCTATATTTTTCTTG‑ATCGGAATTTTTGTTGCAATATACGGTATGAAGCGAGCAACATATTGGTTAAACTTATTGATTTTATTTACCAATAtt     >  1:2373219/1‑100 (MQ=255)                                                                           cATAGGGGCTATATTTTTCTTG‑ATCGGAATTTTTGTTGCAATATACGGTATGAAGCGAGCAACATATTGGTTAAACTTATTGATTTTATTTACCAATAtt     >  1:2759519/1‑100 (MQ=255)                                                                           cATAGGGGCTATATTTTTCTTG‑ATCGGAATTTTTGTTGCAATATACGGTATGAAGCGAGCAACATATTGGTTAAACTTATTGATTTTATTTACCAATAtt     >  1:3372209/1‑100 (MQ=255)                                                                           agcaGGGGCTATATTTTTCTTG‑ATCGGAATTTTTGTTGCAATATACGGTATGAAGCGAGCAACATATTGGTTAAACTTATTGATTTTATTTACCAATAtt     >  1:1865907/4‑100 (MQ=255)                                                                            aTAGGGGCTATATTTTTCTTG‑ATCGGAATTTTTGTTGCAATATACGGTATGAAGCGAGCAACATATTGGTTAAACTTATTGATTTTATTTACCAATAttt    >  1:5912571/1‑100 (MQ=255)                                                                             tAGGGGCTATATTTTTCTTG‑ATCGGAATTTTTGTTGCAATATACGGTATGAAGCGAGCAACATATTGGTTAAACTTATTGATTTTATTTACCAATAtttt   >  1:2695916/1‑100 (MQ=255)                                                                              aGGGGCTATATTTTTCTTG‑ATCGGAATTTTTGTTGCAATATACGGTATGAAGCGAGCAACATATTGGTTAAACTTATTGATTTTATTTACCAATAttttt  >  1:5715089/1‑100 (MQ=255)                                                                              aGGGGCTATATTTTTCTTG‑ATCGGAATTTTTGTTGCAATATACGGTATGAAGCGAGCAACATATTGGTTAAACTTATTGATTTTATTTACCAATAttttt  >  1:6440126/1‑100 (MQ=255)                                                                              aGGGGCTATATTTTTCTTG‑ATCGGAATTTTTGTTGCAATATACGGTATGAAGCGAGCAACATATTGGTTAAACTTATTGATTTTATTTACCAATAttttt  >  1:2739849/1‑100 (MQ=255)                                                                                                |                                                                                   GTCAACTCATTAACTCATTTTCTAACTCTAAACACCTCATTTTTTAATAATTCAGCATCGGATTTCTGTTTTATCATAGGGGCTATATTTTTCTTA‑ATCGGAATTTTTGTTGCAATATACGGTATGAAGCGAGCAACATATTGGTTAAACTTATTGATTTTATTTACCAATATTTTT  >  NC_007793/2085303‑2085479

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑