Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,063,322	A→G	Y238H (TAT→CAT)	SAUSA300_RS10385 ←	sodium‑dependent dicarboxylate transporter SdcS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,063,322	0	A	G	100.0%	84.8 / NA	33	Y238H (TAT→CAT)	SAUSA300_RS10385	sodium‑dependent dicarboxylate transporter SdcS
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (2/31);  total (2/31)

TAAACGCAACATATCTTAAATAGAGCCAAGTAATACCTAACAAAACAATGACCGTTGGAATCCCTACAATCATCCATTTAGCAAAACTAATTTCATATCCAAAATGTTGCATGTATTGTCCTTTTAAAATAATTAATGGCGGGGTTCCGATTAATGTACCCAAGCCACCAATCGTACCTGCATAGCCA  >  NC_007793/2063226‑2063413                                                                                                 |                                                                                            tAAACGCAACATATCTTAAATAGAGCCAAGTAATACCTAACAAAACAATGACCGTTGGAATCCCTACAATCGTCCATTTAGCAAAACTAATTTCATGTcc                                                                                          <  1:980162/100‑1 (MQ=255)    aCGCAACATATCTTAAATAGAGCCAAGTAATACCTAACAAAACAATGACCGTTGGAATCCCTACAATCATCCATTTAGCAAAACTAATTTCATGTCCaag                                                                                       <  1:6574933/100‑2 (MQ=255)      gCAACATATCTTAAATAGAGCCAAGTAATACCTAACAAAACAATGACCGTTGGAATCCCTACAATCATCCATTTAGCAAAACTAATTTCATGTCCacccc                                                                                     <  1:4717151/100‑5 (MQ=255)        aaCATATCTTAAATAGAGCCAAGTAATACCTAACAAAACAATGACCGTTGGAATCCCTACAATCATCCATTTAGCAAAACTAATTTCATGTCCAAAATGt                                                                                   <  1:6317618/100‑1 (MQ=255)           atatCTTAAATAGAGCCAAGTAATACCTAACAAAACAATGACCGTTGGAATCCCTACAATCATCCATTTAGCAAAACTAATTTCATGTCCAAAATGTTGc                                                                                <  1:3033746/100‑1 (MQ=255)             atCTTAAATAGAGCCAAGTAATACCTAACAAAACAATGACCGTTGGAATCCCTACAATCATCCATTTAGCAAAACTAATTTCATGTCCAAAATGTTGcct                                                                              <  1:907353/100‑3 (MQ=255)              tCTTAAATAGAGCCAAGTAATACCTAACAAAACAATGACCGTTGGAATCCCTACAATCATCCATGTAGCAAAACTAATTTCATGTCCAAAATGTTGCATg                                                                             <  1:2358107/100‑1 (MQ=255)               cTTAAATAGAGCCAAGTAATACCTAACAAAACAATGACCGTTGGAATCCCTACAATCATCCATTTAGCAAAACTAATTTCATGTCCAAAATGTTGCATGt                                                                            <  1:6135360/100‑1 (MQ=255)               cTTAAATAGAGCCAAGTAATACCTAACAAAACAATGACCGTTGGAATCCCTACAATCATCCATTTAGCAAAACTAATTTCATGTCCAAAATGTTGCATGt                                                                            <  1:4352808/100‑1 (MQ=255)                ttAAATAGAGCCAAGTAATACCTAACAAAACAATGACCGTTGGAATCCCTACAATCATCCATTTAGCAAAACTAATTTCATGTCCAAAATGTTGCATgca                                                                           <  1:3015189/100‑3 (MQ=255)                                              caaTGACCGTTGGAATCCCTACAATCATCCATTTAGCAAAACTAATTTCATGTCCAAAATGTTGCATGTATTGTCCTTTTAAAATAATTAATGGCgggtg                                             <  1:5436927/100‑3 (MQ=255)                                              caaTGACCGTTGGAATCCCTACAATCATCCATTTAGCAAAACTAATTTCATGTCCAAAATGTTGCATGTATTGTCCTTTTAAAATAATTAATGGCGGGGt                                             <  1:6244192/100‑1 (MQ=255)                                              caaTGACCGTTGGAATCCCTACAATCATCCATTTAGCAAAACTAATTTCATGTCCAAAATGTTGCATGTATTGTCCTTTTAAAATAATTAATGGCGGGGt                                             <  1:5604273/100‑1 (MQ=255)                                               aaTGACCGTTGGAATCCCTACAATCATCCATTTAGCAAAACTAATTTCATGTCCAAAATGTTGCATGTATTGTCCTTTTAAAATAATTAATGGCGGGGtt                                            <  1:3422745/100‑1 (MQ=255)                                                aTGACCGTTGGAATCCCTACAATCATCCATTTAGCAAAACTAATTTCATGTCCAAAATGTTGCATGTATTGTCCTTTTAAAATAATTAATGGCGGGGGTc                                           >  1:4075947/1‑100 (MQ=255)                                                              cccTACAATCATCCATTTAGCAAAACTAATTTCATGTCCAAAACGTTGCATGTATTGTCCTGTTAAAATAATTAATGGCGGGGTTCCGATTAATGTAccc                             <  1:6116717/100‑1 (MQ=255)                                                               ccTACAATCATCCATTTAGCAAAACTAATTTCATGTCCAAAATGTTGCATGTATTGTCCTTTTAAAATAATTAATGGCGGGGTTCCGATTAATGTACCCa                            <  1:5700156/100‑1 (MQ=255)                                                                 tACAATCATCCATTTAGCAAAACTAATTTCATGTCCAAAATGTTGCATGTATTGTCCTTTTAAAATAATTAATGGCGGGGTTCCGATTAATGTACCCagg                          <  1:4387898/100‑3 (MQ=255)                                                                         tcCATTTAGCAAAACTAATTTCATGTCCAAAATGTTGCATGTATTGTCCTTTTAAAATAATTAATGGCGGGGTTCCGATTAATGTACCCAAGCCACCaag                  <  1:4221152/100‑2 (MQ=255)                                                                              ctAGCAAAACTAATTTCATGTCCAAAATGTTGCATGTATTGTCCTTTTAAAATAATTAATGGCGGGGTTCCGATTAATGTACCCAAGCCACCAAACCTAc             >  1:5879728/2‑100 (MQ=255)                                                                               tAGCAAAACTAATTTCATGTCCAAAATGTTGCATGTATTGTCCTTTTAAAATAATTAATGGCGGGGTTCCGATTAATGTACCCAAGCCACCAATCGTAca            <  1:3144550/100‑2 (MQ=255)                                                                                 gCAAAACTAATTTCATGTCCAAAATGTTGCATGTATTGTCCTTTTAAAATAATTAATGGCGGGGTTCCGATTAATGTACCCAAGCCACCAATCGTACCTg          <  1:4680079/100‑1 (MQ=255)                                                                                 gCAAAACTAATTTCATGTCCAAAATGTTGCATGTATTGTCCTTTTAAAATAATTAATGGCGGGGTTCCGATTAATGTACCCAAGCCACCAATCGTACCTg          <  1:3342594/100‑1 (MQ=255)                                                                                 gCAAAACTAATTTCATGTCCAAAATGTTGCATGTATTGTCCTTTTAAAATAATTAATGGCGGGGTTCCGATTAATGTACCCAAGCCACCAATCGTACCTg          <  1:6652670/100‑1 (MQ=255)                                                                                  cAAAACTAATTTCATGTCCAAAATGTTGCATGTATTGTCCTTTTAAAATAATTAATGGCGGGGTTCCGATTAATGTACCCAAGCCACCAATCGTACCTGc         <  1:3680746/100‑1 (MQ=255)                                                                                  cAAAACTAATTTCATGTCCAAAATGTTGCATGTATTGTCCTTTTAAAATAATTAATGGCGGGGTTCCGATTAATGTACCCAAGCCACCAATCGTACCTGc         <  1:3659590/100‑1 (MQ=255)                                                                                    aaaCTAATTTCATGTCCAAAATGTTGCATGTATTGTCCTTTTAAAATAATTAATGGCGGGGTTCCGTTTAATGTACCCAAGCCACCAATCGTACCTGCAt       <  1:268566/100‑1 (MQ=255)                                                                                    aaaCTAATTTCATGTCCAAAATGTTGCATGTATTGTCCTTTTAAAATAATTAATGGCGGGGTTCCGATTAATGTACCCAAGCCACCAATCGTACCTGCAt       <  1:2178664/100‑1 (MQ=255)                                                                                     aaCTAATTTCATGTCCAAAATGTTGCATGTATTGTCCTTTTAAAATAATTAATGGCGGGGTTCCGATTAATGTACCCAAGCCACCAATCGGACCTGCATa      <  1:3650519/100‑1 (MQ=255)                                                                                        tAATTTCATGTCCAAAATGTTGCATGTATTGTCCTTTTAAAATAATTAATGGCGGGGTTCCGATTAATGTACCCAAGCCACCAATCGTACCTGCATAggc   <  1:798838/100‑3 (MQ=255)                                                                                        tAATTTCATGTCCAAAATGTTGCATGTATTGTCCTTTTAAAATAATTAATGGCGGGGTTCCGATTAATGTACCCAAGCCACCAATCGTACCTGCATAGcc   <  1:5394528/100‑1 (MQ=255)                                                                                         aaTTTCATGTCCAAAATGTTGCATGTATTGTCCTTTTAAAATAATTAATGGCGGGGTTCCGATTAATGTACCCAAGCCACCAATCGTACCTGCAtgatca  <  1:3056225/100‑6 (MQ=255)                                                                                         aaTTTCATGTCCAAAATGTTGCATGTATTGTCCTTTTAAAATAATTAATGGCGGGGTTCCGATTAATGTACCCAAGCCACCAATCGTACCTGCATAGCCa  <  1:1683787/100‑1 (MQ=255)                                                                                                 |                                                                                            TAAACGCAACATATCTTAAATAGAGCCAAGTAATACCTAACAAAACAATGACCGTTGGAATCCCTACAATCATCCATTTAGCAAAACTAATTTCATATCCAAAATGTTGCATGTATTGTCCTTTTAAAATAATTAATGGCGGGGTTCCGATTAATGTACCCAAGCCACCAATCGTACCTGCATAGCCA  >  NC_007793/2063226‑2063413

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑