Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,057,596	T→C	I20V (ATT→GTT)	SAUSA300_RS10355 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,057,596	0	T	C	100.0%	52.4 / NA	22	I20V (ATT→GTT)	SAUSA300_RS10355	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (0/22);  total (0/22)

ATACTTCTAAAAAACTAACTGATGCAGCAATATAACGATAACCTTTCAACGTTAAAATTGTTCGCATCGTTAAAAACGTTACATAACAAATGTTAATGATAAATATAGTTAGTACCATTAACCATGGATTTTCTGTTACGAATGACATAAGGCGCCCCACTCTCTTTCAATTACACTTTAAATACCA  >  NC_007793/2057506‑2057692                                                                                           |                                                                                                 gatcTTCTAAAAAACTAACTGATGCAGCAATATAACGATAACCTTTCAACGTTAAAATTGTTCGCATCGTTAAAAACGTTACATAACAAACGTTAATGAt                                                                                         <  1:5596589/97‑1 (MQ=255)  tACTTCTAAAAAACTAACTGATGCAGCAATATAACGATAACCTTTCAACGTTAAAATTGTTCGCATCGTTAAAAACGTTACATAACAAACGTTAATGATa                                                                                        <  1:1539180/100‑1 (MQ=255)  tACTTCTAAAAAACTAACTGATGCAGCAATATAACGATAACCTTTCAACGTTAAAACTGTTCGCATCGTTAAAAACGTTACATAACAAACGTTAATgctg                                                                                        <  1:337055/100‑4 (MQ=255)           aaaaCTAACTGATGCAGCAATATAACGATAGCCTTTCAACGTTAAAATTGTTCGCATCGTTAAAAACGTTACATAACAGACGTTAATGATAAATAtagtt                                                                               <  1:1211844/100‑1 (MQ=255)           aaaaCTAACTGATGCAGCAATATAACGATAACCTTTCAACGTTAAAATTGTTCGCATCGTTAAAAACGTTACATAACAAACGTTAATGATAAATAtagtt                                                                               <  1:2477471/100‑1 (MQ=255)           aaaaCTAACTGATGCAGCAATATAACGATAACCTTTCAACGTTAAAATTGTTCGCATCGTTAAAAACGTTACATAACAAACGTTAATGATAAATAtagtt                                                                               <  1:2873509/100‑1 (MQ=255)           aaaaCTAACTGATGCAGCAATATAACGATAACCTTTCAACGTTAAAATTGTTCGCATCGTTAAAAACGTTACATAACAAACGTTAATGATAAATAtagtt                                                                               <  1:3833925/100‑1 (MQ=255)                    tGATGCAGCAATATAACGATAACCTTTCAACGTTAAAATTGTTCGCATCGTTAAAAACGTTACATAACAAACGTTAATGATAAATATAGTTAGTACCAtt                                                                      <  1:3236239/100‑1 (MQ=255)                            caATATAACGATAATCTTTCAACGTTAAAATTGTTCGCATCGTTAAAAACGTTACATAACAAACGTTAATGATAAATATAGTTAGTACCATTAACCATgg                                                              <  1:4807007/100‑1 (MQ=255)                            caATATAACGATAACCTTTCAACGTTAAAATTGTTCGCATCGTTAAAAACGTTACATAACAAACGTTAATGATAAATGTAGTTAGTACCATTAACCATgg                                                              <  1:3087651/100‑1 (MQ=255)                            caATATAACGATAACCTTTCAACGTTAAAATTGTTCGCATCGTTAAAAACGTTACATAACAAACGTTAATGATAAATATAGTTAGTACCATTAACCATgg                                                              <  1:1600935/100‑1 (MQ=255)                               tataACGATAACCTTTCAACGTTAAAATTGTTCGCATCGTTAAAAACGTTACATAACAAACGTTAATGATAAATATAGTTAGTACCATTAACCATGGAtt                                                           <  1:4516537/100‑1 (MQ=255)                                                     tAAAATTGTTCGCATCGTTAAAAACGTTACATAACAAACGTTAATGATAAATATAGTTAGTACCATTAACCATGGATTTTCTGTTACGAATGACATAAgg                                     <  1:3804486/100‑1 (MQ=255)                                                        aaTTGTTCGCATCGTTAAAAACGTTACATAACAAACGTTAATGATAAATATAGTTAGTACCATTAACCATGGATTTTCTGTTACGAATGACATAAGgcag                                  <  1:1590894/100‑3 (MQ=255)                                                         actGTTCGCATCGTTAAAAACGTTACATAACAAACGTTAATGATAAATATAGTTAGTACCATTAACCATGGATTTTCTGTTACGAATGACATAAGGGGcc                                 <  1:6033120/98‑1 (MQ=255)                                                         aatGTTCGCATCGTTAAAAACGTTACATAACAAACGTTAATGATAAATATAGTTAGTACCATTAACCATGGATTTTCTGTTACGAATGACATAAGgagca                                 <  1:58433/98‑2 (MQ=255)                                                               cGCATCGTTAAAAATGTTACATAACAAACGTTAATGATAAATATAGTTAGTACCATTAACCATGGATTTTCTGTTACGAATGACATAAGGCGCCCCAcaa                           <  1:4963476/100‑3 (MQ=255)                                                               cGCATCGTTAAAAACGTTACATAACAAACGTTAATGATAAATATAGTTAGTACCATTAACCATGGATTTTCTGTTACGAATGACATAAGGCGCCCCActg                           <  1:1290103/100‑2 (MQ=255)                                                                gCATCGTTAAAAACGTTACATAACAAACGTTAATGATAAATATAGTTAGTACCATTAACCATGGATTTTCTGTTACGAATGACATAAGGCGCCCCActct                          <  1:5591422/100‑1 (MQ=255)                                                                     gTTAAAAACGTTACATAACAAACGTTAATGATAAATATAGTTAGTACCATTAACCATGGATTTTCTGTTACGAATGACATAAGGCGCCCCACTCTCTTTc                     <  1:396794/100‑1 (MQ=255)                                                                      ttAAAAACGTTACATAACAAACGTTAATGATAAATATAGTTAGTACCATTAACCATGGATTTTCTGTTACGAATGACATAAGGCGCCCCACTCTCTTTCa                    <  1:4056626/100‑1 (MQ=255)                                                                                        aaaCGTTAATGATAAATATAGTTAGTACCATTAACCATGGATTTTCTGTTACGAATGACATAAGGCGCCCCACTCTCTTTCAATTACACTTTAAATACCa  <  1:4198147/100‑1 (MQ=255)                                                                                           |                                                                                                 ATACTTCTAAAAAACTAACTGATGCAGCAATATAACGATAACCTTTCAACGTTAAAATTGTTCGCATCGTTAAAAACGTTACATAACAAATGTTAATGATAAATATAGTTAGTACCATTAACCATGGATTTTCTGTTACGAATGACATAAGGCGCCCCACTCTCTTTCAATTACACTTTAAATACCA  >  NC_007793/2057506‑2057692

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑