Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,034,247	G→T	intergenic (‑177/‑115)	SAUSA300_RS10245 ← / → SAUSA300_RS10250	UDP‑N‑acetylmuramate‑‑alanine ligase/non‑heme ferritin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,034,247	0	G	T	100.0%	17.6 / NA	8	intergenic (‑177/‑115)	SAUSA300_RS10245/SAUSA300_RS10250	UDP‑N‑acetylmuramate‑‑alanine ligase/non‑heme ferritin
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (0/8);  total (0/8)

GTGTTTCTAAATTATAATCATTCTCATTTAGATAATTAAAAATGATATTTATTCTCAATTAAATATCGCTAAATGATACATTTTCTCAATTAGAGTGCTAAAATAAGGCATTAAATTATAATTATTATTATTTACAATTGAGAATGAGTTTGTTATACTAAGTTTAATTCAT  >  NC_007793/2034155‑2034326                                                                                             |                                                                                gtgtTTCTAAATTATAATCATTCTCATTTAGATAATTAAAAATGATATTTATTCTCAATTAAATATCGCTAAATGATACATTTTCTCAATTATAGTGCTa                                                                          <  1:6054054/100‑1 (MQ=255)          aaTTATAATCATTCTCATTTAGATAATTAAAAATGATATTTATTCTCAATTAAATATCGCTAAATGATACATTTTCTCAATTATAGTGCTAAAATAAGGc                                                                 <  1:6283545/100‑1 (MQ=255)          aaTTATAATCATTCTCATTTACATAATTAAAAATGATATTTATTCTCAATTAAATATCGCTAAATGATACATTTTCTCAATTATAGTGCTAAAATAAGGc                                                                 <  1:4372347/100‑1 (MQ=255)              ataATCATTCTCATTTAGATAATTAAAAATGATATTTATTCTCAATTAAATATCGCTAAATGATACATTTTCTCAATTATAGTGCTAAAATAAGGCATTa                                                             <  1:2488370/100‑1 (MQ=255)              ataATCATTCTCATTTAGATAATTAAAAATGATATTTATTCTCAATTAAATATCGCTAAATGATACATTTTCTCAATTATAGTGCTAAAATAAGGCATTa                                                             <  1:3643094/100‑1 (MQ=255)               taATCATTCTCATTTAGATAATTAAAAATGATATTTATTCTCAATTAAATATCGCTAAATGATACATTTTCTCAATTATAGTGCTAAAATAAGGCATTaa                                                            <  1:3271769/100‑1 (MQ=255)               taATCATTCTCATTTAGATAATTAAAAATGATATTTATTCTCAATTAAATATCGCTAAATGATACATTTTCTCAATTATAGTGCTAAAATAAGGCATTaa                                                            <  1:5421041/100‑1 (MQ=255)                                                                         aTGATACATTTTCTCAATTATAGTGCTAAAATAAGGCATTAAATTATAATTATTATTATTTACAATTGAGAATGAGTTTGTTATACTAAGTTTAATTCAt  <  1:4150363/100‑1 (MQ=255)                                                                                             |                                                                                GTGTTTCTAAATTATAATCATTCTCATTTAGATAATTAAAAATGATATTTATTCTCAATTAAATATCGCTAAATGATACATTTTCTCAATTAGAGTGCTAAAATAAGGCATTAAATTATAATTATTATTATTTACAATTGAGAATGAGTTTGTTATACTAAGTTTAATTCAT  >  NC_007793/2034155‑2034326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑