Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	356,294	T→C	S171G (AGT→GGT)	SAUSA300_RS01630 ←	branched‑chain amino acid transporter II carrier protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	356,294	0	T	C	100.0%	209.2 / NA	76	S171G (AGT→GGT)	SAUSA300_RS01630	branched‑chain amino acid transporter II carrier protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (2/74);  total (2/74)

GAAAAAGCTAACGCAGCAACAAGATCCATTGTAAAATAGCCTTCCAAACTTCCTGAAATGAAAGGATGTGTTATATATTTATCCTTAGGTGCACTTAGTGCAGATTCAGGGTTGAAAATGACAGCAATACTTAATAGAGCGACCATTAATAGTAATAACGGTGTTAATAATTTACCTAAAT  >  NC_007793/356200‑356380                                                                                               |                                                                                       gAAAAAGCTAACGCAGCAACAAGATCCATTGTAAAATAGCCTTCCAAACTTCCTGAAATGAAAGGATGTGTTATATATTTATCCTTAGGTGCACCTAGTg                                                                                   <  1:1232430/100‑1 (MQ=255)  aaaaaGCTAACGCAGCAACAAGATCCATTGTAAAATAGCCTTCCAAACTTCCTGAAATGAAAGGATGTGTTATATATTTATCCTTAGGTGCACCTAGTGc                                                                                  <  1:1457749/100‑1 (MQ=255)  aaaaaGCTAACGCAGCAACAAGATCCATTGTAAAATAGCCTTCCAAACTTCCTGAAATGAAAGGATGTGTTATATATTTATCCTTAGGTGCACCTAGTGc                                                                                  <  1:5247759/100‑1 (MQ=255)  aaaaaGCTAACGCAGCAACAAGATCCATTGTAAAATAGCCTTCCAAACTTCCTGAAATGAAAGGATGTGTTATATATTTATCCTTAGGTGCACCTAGTGc                                                                                  <  1:5517918/100‑1 (MQ=255)    aaaGCTAACGCAGCAACAAGATCCATTGTAAAATAGCCTTCCAAACTTCCTGAAATGAAAGGATGTGTTATATATTTATCCTTAGGTGCACCTAGTGCAg                                                                                <  1:1137993/100‑1 (MQ=255)               agcaACAAGATCCATTGTAAAATAGCCTTCCAAACTTCCTGAAATGAAAGGATGTGTTATATATTTATCCTGAGGTGCACCTAGTGCAGATTCAGGGTTg                                                                     <  1:3150283/100‑1 (MQ=255)                gcaACAAGATCCATTGTAAAATAGCCTTCCAAACTTCCTGAAATGAAAGGATGTGTTATATATTTATCCTTAGGTGCACCTAGTGCAGATTCAGGGTTgt                                                                    <  1:3999237/100‑2 (MQ=255)                       gATCCATTGTAAAATAGCCTTTCAAACTGCCTGAAATGAAAGGATGCGTTATATATTTATCCTTAGGTGCACCTAGTGCAGATTCAGGGTTGAAAATGAc                                                             <  1:4445547/100‑1 (MQ=255)                           taTTGTAAAATAGCCTTCCAAACTTCCTGAAATGAAAGGATGTGTTATATATTTATCCTTAGGTGCACCTAGTGCAGATTCAGGGTTGAAAATGACAGCa                                                         <  1:6440315/99‑1 (MQ=255)                           cATTGTAAAATAGCCTTCCAAACTTCCTGAAATGAAAGGATGTGTTATATATTTATCCTTAGGTGCACCTAGTGCAGATTCAGGGTTGAAAATGACAGCa                                                         <  1:4570493/100‑1 (MQ=255)                                    gtgctcTTCCGATCTTCCTGGAATGAAAGGATGTGTTATATATTTATCCTTAGGTGCACCTAGTGCAGATTCAGGGTTGAAAATGACAGCAATACTTAAt                                                <  1:5237363/95‑1 (MQ=255)                                    gtgctcTTCCGATCTTCCTGCAATGAAAGGATGTGTTATATATTTATCCTTAGGTGCACCTAGTGCAGATTCAGGGTTGAAAATGACAGCAATACTTAAt                                                <  1:1076028/95‑1 (MQ=255)                                    gtgctcTTCCGATCTTCCTGAAATGAAAGGATGTGTTATATATTTATCCTTAGGTGCACCTAGTGCAGATTCAGGGTTGAAAATGACAGCAATACTTAAt                                                <  1:1586990/95‑1 (MQ=255)                                    aTAGCCTTCCAAACTTCCTGAAATGAAAGGATGTGTTATATATTTATCCTTAGGTGCACCTAGTGCAGATTCAGGGTTGAAAATGACAGCAATACTTAAt                                                <  1:3343551/100‑1 (MQ=255)                                    aTAGCCTTCCAAACTTCCTGAAATGAAAGGATGTGTTATATATTTATCCTTAGGTGCACCTAGTGCAGATTCAGGGTTGAAAATGACAGCAATACTTAAt                                                <  1:6343802/100‑1 (MQ=255)                                    aTAGCCTTCCAAACTTCCTGAAATGAAAGGATGTGTTATATATTTATCCTTAGGTGCACCTAGTGCAGATTCAGGGTTGAAAATGACAGCAATACTTAAt                                                <  1:774733/100‑1 (MQ=255)                                    aTAGCCTTCCAAACTTCCTGAAATGAAAGGATGTGTTATATATTTATCCTTAGGTGCACCTAGTGCAGATTCAGGGTTGAAAATGACAGCAATACTTAAt                                                <  1:2236124/100‑1 (MQ=255)                                            cgatcttcccTGAAATGAAAGGATGTGTTATATATTTATCCTTAGGTGCACCTAGTGCAGATTCAGGGTTGAAAATGACAGCAATACTTAATAGAGCgtc                                        <  1:3918080/92‑3 (MQ=255)                                                tcTTCCTGAAATGAAAGGATGTGTTATATATTTATCCTTAGGTGCACCTAGTGCAGATTCAGGGTTGAAAATGACAGCAATACTTAATAGAGCGACCAtt                                    <  1:4039853/99‑1 (MQ=255)                                                   tacttaaatgaaaGGATGTGTTATATATTTATCCTTAGGTGCACCTAGTGCAGATTCAGGGTTGAAAATGACAGCAATACTTAATAGAGCGACCATTAAt                                 >  1:22791/6‑100 (MQ=255)                                                   tCCTGTAATGAAAGGATGTGTTATAAATTTAACCTTAGGTACACCTAGTGCAGATTCAGGGTTGAAAATGACAGCAATACTTAATAGAGCGACCATTAAt                                 <  1:4638479/100‑1 (MQ=255)                                                   tCCTGGCATGAAAGGATGTGTTATATATTAATCCTTAGGTGCACCTAGTGCAGATTCAGGGTTGAAAATGACAGCAATACTTAATAGAGCGACCATTAAt                                 <  1:4587720/100‑1 (MQ=255)                                                   tCCTGAAATGAAAGGATGTGTTATATATTTATCCTTAGGTGCACCTAGTGCAGATTCAGGGTTGAAAATGACAGCAATGCTTAATAGAGCGACCATTAAt                                 <  1:1793979/100‑1 (MQ=255)                                                   tCCTGAAATGAAAGAATGTGTTATATATTTATCCTTAGGTGCACCTAGTGCAGATTCAGGGTTGAAAATGACAGCAATACTTAATAGATCggaagagcac                                 >  1:6250591/1‑91 (MQ=255)                                                   tCCTCAAATGAAAGGATGTGTTATATATTTATCCTTAGGTGCACCTAGTGCAGATTCAGGGTTGAAAATGACAGCAATACTTAATAGAGCGACCATTAAt                                 <  1:125713/100‑1 (MQ=255)                                                   accTGAAATGAAAGGATGTGTTATATATTTATCCTTAGGTGCACCTAGTGCAGATTCAGGGTTGAAAATGACAGCAATACTTAATAGAGCGACCATTAAt                                 <  1:5713800/99‑1 (MQ=255)                                                      tgtaatgaaaGGATGTGTTATATATTTATCCTTAGGTGCACCTAGTGCAGATTCAGGGTTGAAAATGACAGCAATACTTAATAGAGCGACCATTAATAgc                              <  1:2245679/97‑2 (MQ=255)                                                      tgaaatgaaaGGATGTGTTATATATTTATCCTTAGGTGCACCTAGTGCAGATTCAGGGTTGAAAATGACAGCAATACTTAATAGAGCGACCATTAATAgg                              <  1:2686285/100‑2 (MQ=255)                                                        caatgaaaGGATGTGTTATATATTTATCCTTAGGTGCACCTAGTGCAGATTCAGGGTTGAAAATGACAGCAATACTTAATAGAGCGACCATTAATAGtat                            <  1:1198437/99‑2 (MQ=255)                                                        aaatgaaaGGATGTGTTATATATTTATCCTTAGGTGCACCTAGTGCAGATTCAGGGTTGAAAATGACAGCAATACTTAATAGAGCGACCATTAATAGtat                            <  1:1731366/100‑2 (MQ=255)                                                         cgatcttggATGGGTTATATATTTATCCTTAGGTGCACCTAGTGCAGATTCAGGGTTGAAAATGACAGCAATACTTAATAGAGCGACCATTAATAGtaat                           <  1:433845/93‑1 (MQ=255)                                                         aatgaaaGGATGTGTTATATATTTATCCTTAGGTGCACCTAGTGCAGATTCAGGGTTGAAAATGACAGCAATACTTAATAGAGCGACCATTAATAGtaat                           <  1:1073102/100‑1 (MQ=255)                                                          atgaaaGGATGTGTTATATATTTATCCTTAGGTGCACCTAGTGCAGATTCAGGGTTGAAAATGACAGCAATACTTAATAGAGCGACCATTAATAGtaata                          <  1:3366758/100‑1 (MQ=255)                                                           tgaaaGGATGTGTTATATATTTATCCTTAGGTGCACCTAGTGCAGATTCAGGGTTGAAAATGACAGCAATACTTAATAGAGCGACCATTAATAgggctag                         <  1:83006/100‑7 (MQ=255)                                                           tgaaaGGATGTGTTATATATTTATCCTTAGGTGCACCTAGTGCAGATTCAGGGTTGAAAATGACAGCAATACTTAATAGAGCGACCATTAATAGtaatag                         <  1:3282244/100‑2 (MQ=255)                                                                ggATGTGTTATATATTTATCCTTAGGTGCACCTAGTGCAGATTCAGGGTTGAAAATGACAGCAATACTTAATAGAGCGACCATTAATAGTAATAACGgtg                    <  1:506146/100‑1 (MQ=255)                                                                ggATGTGTTATATATTTATCCTTAGGTGCACCTAGTGCAGATTCAGGGTTGAAAATGACAGCAATACTTAATAGAGCGACCATTAATAGTAATAACGgtg                    <  1:3104896/100‑1 (MQ=255)                                                                 gATGTGTTATATATTTATCCTTAGGTGCACCTAGTGCAGATTCAGGGTTGAAAATGACAGCAATACTTAATAGAGCGACCATTAATAGTAATAACgggcc                   <  1:6584507/100‑4 (MQ=255)                                                                 gATGTGTTATATATTTATCCTTAGGTGCACCTAGTGCAGATTCAGGGTTGAAAATGACAGCAATACTTAATAGAGCGACCATTAATAGTAATAACGgtgt                   <  1:2722638/100‑1 (MQ=255)                                                                 gATGTGTTATATATTTATCCTTAGGTGCACCTAGTGCAGATTCAGGGTTGAAAATGACAGCAATACTTAATAGAGCGACCATTAATAGTAATAACGgtgt                   <  1:6121315/100‑1 (MQ=255)                                                                 gATGTGTTATATATTTATCCTTAGGTGCACCTAGTGCAGATTCAGGGTTGAAAATGACAGCAATACTTAATAGAGCGACCATTAATAGTAATAACGgtgt                   <  1:5147721/100‑1 (MQ=255)                                                                 gATGTGTTATATATTTATCCTTAGGTGCACCTAGTGCAGATTCAGGGTTGAAAATGACAGCAATACTTAATAGAGCGACCATTAATAGTAATAACGgtgt                   <  1:512960/100‑1 (MQ=255)                                                                 gATGTGTTATATATTTATCCTTAGGTGCACCTAGTGCAGATTCAGGGTTGAAAATGACAGCAATACTTAATAGAGCGACCATTAATAGTAATAACGgtgc                   <  1:1150475/100‑2 (MQ=255)                                                                 gATGTGTTATATATTTATCCTTAGGTGCACCTAGTGCAGATTCAGGGTTGAAAATGACAGCAATACTTAATAGAGCGACCATTAATAGTAATAACGgggt                   <  1:2782731/100‑1 (MQ=255)                                                                 gATGTGTTATATATTTATCCTTAGGTGCACCTAGTGCAGATTCAGGGTTGAAAATGACAGCAATACTTAATAGAGCGACCATTAATAGTAATAACGgggt                   <  1:1780471/100‑1 (MQ=255)                                                                 gATGTGTTATATAGTTATCCTTAGGTGCACCTAGTGCAGATTCAGGGTTGAAAATGACAGCAATACTTAATAGAGCGACCATTAATAGTAATAACGgtgt                   <  1:3545082/100‑1 (MQ=255)                                                                  aTGTGTTATATATTTATCCTTAGGTGCACCTAGTGCAGATTCAGGGTTGAAAATGACAGCAATACTTAATAGAGCGTCCATTAATAGTAATAACGGTGtt                  <  1:5441673/100‑1 (MQ=255)                                                                  aTGTGTTATATATTTATCCTTAGGTGCACCTAGTGCAGATTCAGGGTTGAAAATGACAGCAATACTTAATAGAGCGGCCATTAATAGTAATAACGGTGtt                  <  1:1769691/100‑1 (MQ=255)                                                                  aTGTGTTATATATTTATCCTTAGGTGCACCTAGTGCAGATTCAGGGTTGAAAATGACAGCAATACTTAATAGAGCGACCATTAATAGTAATAACGGTGtt                  <  1:77468/100‑1 (MQ=255)                                                                  aTGTGTTATATATTTATCCTTAGGTGCACCTAGTGCAGATTCAGGGTTGAAAATGACAGCAATACTTAATAGAGCGACCATTAATAGTAATAACGGTGtt                  <  1:6286793/100‑1 (MQ=255)                                                                  aTGTGTTATATATTTATCCTTAGGTGCACCTAGTGCAGATTCAGGGTTGAAAATGACAGCAATACTTAATAGAGCGACCATTAATAGTAATAACGGTGtt                  <  1:1671759/100‑1 (MQ=255)                                                                  aTGTGTTATATATTTATCCTTAGGTGCACCTAGTGCAGATTCAGGGTTGAAAATGACAGCAATACTTAATAGAGCGACCATTAATAGTAATAACGGTGtt                  <  1:529709/100‑1 (MQ=255)                                                                  aTGTGTTATATATTTATCCTTAGGTGCACCTAGTGCAGATTCAGGGTTGAAAATGACAGCAATACTTAATAGAGCGACCATTAATAGTAATAACGGTGtt                  <  1:1110652/100‑1 (MQ=255)                                                                  aTGTGTTATATATTTATCCTTAGGTGCACCTAGTGCAGATTCAGGGTTGAAAATGACAGCAATACTTAATAGAGCGACCATTAATAGTAATAACGGTGtt                  <  1:5074910/100‑1 (MQ=255)                                                                  aTGTGTTATATATTTATCCTTAGGTGCACCTAGTGCAGATTCAGGGTTGAAAATGACAGCAATACTTAATAGAGCGACCATTAATAGTAATAACGGTGtt                  <  1:3986307/100‑1 (MQ=255)                                                                  aTGTGTTATATATTTATCCTTAGGTGCACCTAGTGCAGATTCAGGGTTGAAAATGACAGCAATACTTAATAGAGCGACCATTAATAGTAATAACGGTGtt                  <  1:306390/100‑1 (MQ=255)                                                                  aTGTGTTATATATTTATCCTTAGGTGCACCTAGTGCAGATTCAGGGTTGAAAATGACAGCAATACTTAATAGAGCGACCATTAATAGTAATAACGGTGtt                  <  1:4250678/100‑1 (MQ=255)                                                                  aTGTGTTATATATTTATCCTTAGGTGCACCTAGTGCAGATTCAGGGTTGAAAATGACAGCAATACTTAATAGAGCGACCATTAATAGTAATAACGGTGtt                  <  1:4463780/100‑1 (MQ=255)                                                                  aTGTGTTATATATTTATCCTTAGGTGCACCTAGTGCAGATTCAGGGTTGAAAATGACAGCAATACTTAATAGAGCGACCATTAATAGTAATAACGGTGtt                  <  1:2450769/100‑1 (MQ=255)                                                                   tgtgTTATATATTTATCCTTAGGTGCACCTAGTGCAGATTCAGGGTTGAAAATGACAGCAATACTTAATAGAGCGACCATTAATAGTAATAACGgggtag                 <  1:1557246/100‑3 (MQ=255)                                                                   tgtgTTATATATTTATCCTTAGGTGCACCTAGTGCAGATTCAGGGTTGAAAATGACAGCAATACTTAATAGAGCGACCATTAATAGTAATAACGGTGttg                 <  1:2164399/100‑2 (MQ=255)                                                                   tgtgTTATATATTTATCCTTAGGTGCACCTAGTGCAGATTCAGGGTTGAAAATGACAGCAATACTTAATAGAGCGACCATTAATAGTAATAACGGTGttg                 <  1:1467963/100‑2 (MQ=255)                                                                   tgtgTTATATATTTATCCTTAGGTGCACCTAGTGCAGATTCAGGGTTGAAAATGACAGCAATACTTAATAGAGCGACCATTAATAGTAATAACGGTGTta                 <  1:6066900/100‑1 (MQ=255)                                                                   tgtgTTATATATTTATCCTTAGGTGCACCTAGTGCAGATTCAGGGTTGAAAATGACAGCAATACTTAATAGAGCGACCATTAATAGTAATAACGGTGTta                 <  1:6414289/100‑1 (MQ=255)                                                                   tgtgTTATATATTTATCCTTAGGTGCACCTAGTGCAGATTCAGGGTTGAAAATGACAGCAAGACTTAATAGAGCGACCATTAATAGTAATAACGGTGTta                 <  1:5862409/100‑1 (MQ=255)                                                                    gtgtTATATATTTATCCTTAGGTGCACCTAGTGCAGATTCAGGGTTGAAAGTGACAGCAATACTTAATAGAGCGACCATTAATAGTAATAACGGTGTtag                <  1:6139406/100‑2 (MQ=255)                                                                     tgtTATATATTTATCCTTAGGTGCACCTAGTGCAGATTCAGGGTTGAAAATGACAGCAATACTTAATAGAGCGACCATTAATAGTAATAACGGTGTtaat               <  1:2406622/100‑1 (MQ=255)                                                                     tgtTATATATTTATCCTTAGGTGCACCTAGTGCAGATTCAGGGTTGAAAATGACAGCAATACTTAATAGAGCGACCATTAATAGTAATAACGGTGTtaat               <  1:3016691/100‑1 (MQ=255)                                                                            tatTTATCCTTAGGTGCACCTAGTGCAGATTCAGGGTTGAAAATGACAGCAATACTTAATAGAGCGACCATTAATAGTAATAACGGTGTTAATAATTTAc        <  1:1976699/100‑1 (MQ=255)                                                                              tttATCCTTAGGTGCACCTAGTGCAGATTCAGGGTTGAAAATGACAGCAATACTTAATAGAGCGACCATTAATAGTAATAACGGTGTTAATAATTTACCt      <  1:2394805/100‑1 (MQ=255)                                                                               ttATCCTTAGGTGCACCTAGTGCAGATTCAGGGTTGAAAATGACAGCAATACTTAATAGAGCGACCATTAATAGTAATAACGGTGTTAATAATTTACCTa     <  1:5744807/100‑1 (MQ=255)                                                                               ttATCCTTAGGTGCACCTAGTGCAGATTCAGGGTTGAAAATGACAGCAATACTTAATAGAGCGACCATTAATAGTAATAACGGTGTTAATAATTTACCTa     <  1:4378987/100‑1 (MQ=255)                                                                               ttATCCTTAGGCGCACCTAGTGCAGATTCAGGGTTGAAAATGACAGCAATACTTAATAGAGCGACCATTAATAGTAATAACGGTGTTAATAATTTACCTa     <  1:4734798/100‑1 (MQ=255)                                                                                  tCCTTAGGTGCACCTAGTGCAGATTCAGGGTTGAAAATGACAGCAATACTTAATAGAGCGACCATTAATAGTAATAACGGTGTTAATAATTTACCTaggt  <  1:4498208/100‑4 (MQ=255)                                                                                  tCCTTAGGTGCACCTAGTGCAGATTCAGGGTTGAAAATGACAGCAATACTTAATAGAGCGACCATTAATAGTAATAACGGTGTTAATAATTTACCTaagc  <  1:3376235/100‑3 (MQ=255)                                                                                  tCCTTAGGTGCACCGAGTGCAGATTCAGGGTTGAAAATGACAGTAATACTTAATAGAGCGACCATTAATAGTAATAACGGTGTTAATAATTTACCTagga  <  1:4864555/100‑4 (MQ=255)                                                                                               |                                                                                       GAAAAAGCTAACGCAGCAACAAGATCCATTGTAAAATAGCCTTCCAAACTTCCTGAAATGAAAGGATGTGTTATATATTTATCCTTAGGTGCACTTAGTGCAGATTCAGGGTTGAAAATGACAGCAATACTTAATAGAGCGACCATTAATAGTAATAACGGTGTTAATAATTTACCTAAAT  >  NC_007793/356200‑356380

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑