Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,015,166	C→T	L287L (TTG→TTA)	SAUSA300_RS10120 ←	teichoic acid ABC transporter ATP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,015,166	0	C	T	100.0%	138.5 / NA	52	L287L (TTG→TTA)	SAUSA300_RS10120	teichoic acid ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (0/52);  total (0/52)

CACGATTCGTAAAATCTGTTATCGAAATAATTCCTATATTATTGAAAATGAGCAACATCATCAATGCTAAGCCTAAAATAGTACCTGAGGCCAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATTATAGCGCTCAACACGTTTGAAATTATAGGTCATCTCTGGTATTCTTGTACGA  >  NC_007793/2015075‑2015262                                                                                            |                                                                                                 cACGATTCGTAAAATCTGTTATCGAAATAATTCCTATATTATTGAAAATGAGCAACATCATCAATGCTAAGCCTAAAATAGTACCTGAGGCTAAAGTCCa                                                                                          <  1:5945101/100‑1 (MQ=255) cACGATTCGTAAAATCTGTTATCGAAATAATTCCTATATTATTGAAAATGAGCAACATCATCAATGCTAAGCCTAAAATAGTACCTGAGGCTAAAGTCCa                                                                                          <  1:1640440/100‑1 (MQ=255) cACGATTCGTAAAATCTGTTATCGAAATAATTCCTATATTATTGAAAATGAGCAACATCATCAATGCTAAGCCTAAAATAGTACCTGAGGCTAAAGTCCa                                                                                          <  1:5344974/100‑1 (MQ=255) cACGATTCGTAAAATCTGTTATCGAAATAATTCCTATATTATTGAAAATGAGCAACATCATCAATGCTAAGCCTAAAATAGTACCTGAGGCTAAAGTCCa                                                                                          <  1:1158596/100‑1 (MQ=255)           aaaaTCTGTTATCGAAATAATTCCTATATTATTGAAAATGAGCAACATCATCAATGCTAAGCCTAAAATAGTACCTGAGGCTAAAGTCCAAAAACGCACt                                                                                <  1:6552163/100‑1 (MQ=255)           aaaaTCTGTTATCGAAATAATTCCTATATTATTGAAAATGAGCAACATCATCAATGCTAAGCCTAAAATAGTACCTGAGGCTAAAGTCCAAAAACGCACt                                                                                <  1:5171679/100‑1 (MQ=255)           aaaaTCTGTTATCGAAATAATTCCTATATTATTGAAAATGAGCAACATCATCAATGCTAAGCCTAAAATAGTACCTGAGGCTAAAGTCCAAAAACGCACt                                                                                <  1:5019686/100‑1 (MQ=255)             aaTCTGTTATCGAAATAATTCCTATATTATTGAAAATGAGCAACATCATCAATGCTAAGCCTAAAGTAGTACCTGAGGCTAAAGTCCAAAAACGCACTaa                                                                              <  1:3074563/100‑1 (MQ=255)             aaTCTGTTATCGAAATAATTCCTATATTATTGAAAATGAGCAACATCATCAATGCTAAGCCTAAAATAGTACCTGAGGCTAAAGTCCAAAAACGCACTaa                                                                              <  1:1257974/100‑1 (MQ=255)                   ttATCGAAATAATTCCTATATTATTGAAAATGAGCAACATCATCAATGCTAAGCCTAAAATAGTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAACtt                                                                        <  1:6289824/100‑1 (MQ=255)                            taatTCCTATATTATTGAAAATGAGCAACATCATCAGTGCTAAGCCTAAAATAGTACCAGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGtac                                                               <  1:5120275/100‑3 (MQ=255)                                     tattatTGAAAATGAGCAACATCATCAATGCTAAGCCTAAAATAGTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGAtt                                                      <  1:22228/100‑1 (MQ=255)                                     tattatTGAAAAAGAGCAACATCATCAATGCTAAGCCTAAAATAGTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGAtt                                                      <  1:422255/100‑1 (MQ=255)                                       ttattGAAAATGAGCAACATCATCAATGCTAAGCCTAAAATAGTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGTTtat                                                    <  1:4277863/100‑1 (MQ=255)                                                      aaCATCATCAATGCTAAGCCTAAAATAGTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATTATAGCGCTCAACACGtt                                     <  1:1632996/100‑1 (MQ=255)                                                          tcatcaATGCTAAGTCTAAAATAGTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATTATAGCGCTCAACACGTTTGaa                                 <  1:4910856/100‑1 (MQ=255)                                                              caATGCTAAGCCTAAAATAGTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATTATAGCGCTCAACACGTTTGAAATta                             <  1:6156225/100‑1 (MQ=255)                                                               aaTGCTAAGCCTAAAATAGTATCTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATTATAGCGCTCAACACGTTTGAAATtat                            <  1:1856489/100‑1 (MQ=255)                                                               aaTGCTAAGCCTAAAATAGTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATTATAGCGCTCAACACGTTTGAAATtat                            <  1:976926/100‑1 (MQ=255)                                                               aaTGCTAAGCCTAAAATAGTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATTATAGCGCTCAACACGTTTGAAATtat                            <  1:5370460/100‑1 (MQ=255)                                                               aaTGCTAAGCCTAAAATAGTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATTATAGCGCTCAACACGTTTGAAATtat                            <  1:2451140/100‑1 (MQ=255)                                                               aaTGCTAAGCCTAAAATAGTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATTATAGCGCTCAACACGTTTGAAATtat                            <  1:603624/100‑1 (MQ=255)                                                                    tAAGCCTAAAATAGTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAGCTTAGGCGGTTTTGCATGATTATAGCGCTCAACACGTTTGAAATTATAGGTc                       <  1:2771484/100‑1 (MQ=255)                                                                    tAAGCCTAAAATAGTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATTATAGCGCTCAACACGTTTGAAATTATAGGTc                       <  1:2636013/100‑1 (MQ=255)                                                                    tAAGCCTAAAATAGTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATTATAGCGCTCAACACGTTTGAAATTATAGGTc                       <  1:6533816/100‑1 (MQ=255)                                                                    tAAGCCTAAAATAGTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATTATAGCGCTCAACACGTTTGAAATTATAGGTc                       <  1:4548426/100‑1 (MQ=255)                                                                    tAAGCCTAAAATAGTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATTATAGCGCTCAACACGTTTGAAATTATAGGTc                       <  1:2996991/100‑1 (MQ=255)                                                                    tAAGCCTAAAATAGTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATTATAGCGCTCAACACGTTTGAAATTATAGGTc                       <  1:4768628/100‑1 (MQ=255)                                                                       gCCTAAAATAGTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATTATAGCGCTCAACACGTTTGAAATTATAGGTCAtc                    <  1:897289/100‑1 (MQ=255)                                                                           aaaaTAGTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGAGTATAGCGCTCAACACGTTTGAAATTATAGGTCAtctcag                <  1:5611038/100‑3 (MQ=255)                                                                             gaTAGTTCCTGAGACTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTAGCATGATTATAGCGCTCAACACGTTTGAAATTATAAGTCATCACTGGt              <  1:3085153/99‑1 (MQ=255)                                                                             aaTAGTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATTATAGCGCTCAACACGTTTGAAATTATAGGTCATCTCTGGt              <  1:1603149/100‑1 (MQ=255)                                                                             aaTAGTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATTATAGCGCTCAACACGTTTGAAATTATAGGTCATCTCTGGt              <  1:5767297/100‑1 (MQ=255)                                                                             aaTAGTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATTATAGCGCTCAACACGTTTGAAATTATAGGTCATCTCTGGt              <  1:16977/100‑1 (MQ=255)                                                                             aaTAGTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATTATAGCGCTCAACACGTTTGAAATTATAGGTCATCTCTGGt              <  1:2359914/100‑1 (MQ=255)                                                                             aaTAGTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATTATAGCGCTCAACACGTTTGAAATTATAGGTCATCTCTGGt              <  1:3054811/100‑1 (MQ=255)                                                                             aaTAGTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATTATAGCGCTCAACACGTTTGAAATTATAGGTCATCTCTGGt              <  1:3210495/100‑1 (MQ=255)                                                                             aaTAGTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAACGTAGGCGGTTTTGCATGATTATAGCGCTCAACACGTTTGAAATTATAGGTCATCTCTGGt              <  1:837779/100‑1 (MQ=255)                                                                              aTAGTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATTATAGCGCTCAACACGTTTGAAATTATAGGTCATCTCTGGTa             <  1:2802912/100‑1 (MQ=255)                                                                              aTAGTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATTATAGCGCTCAACACGTTTGAAATTATAGGTCATCTCTGGTa             <  1:6201936/100‑1 (MQ=255)                                                                              aTAGTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATTATAGCGCTCAACACGTTTGAAATTATAGGTCATCTCTGGTa             <  1:5496605/100‑1 (MQ=255)                                                                               tccgatcTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATTATAGCGCTCAACACGTTTGAAATTATAGGTCATCTCTggggt            <  1:2948923/94‑4 (MQ=255)                                                                               tAGTACCTGAGGCTAAAGTCCAAAAACGCCCTAAAAACTTAGGCGGTTTTGCATGATTATAGCGCCCAACACGTTTGAAATTATAGGTCATCTCTGGTAt            <  1:5642864/100‑1 (MQ=255)                                                                               tAGTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATTATAGCGCTCAACACGTTTGAAATTATAGGTCATCTCTGGTAt            <  1:1359271/100‑1 (MQ=255)                                                                               tAGTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATTATAGCGCTCAACACGTTTGAAATTATAGGTCATCTCTGGTAt            <  1:4741652/100‑1 (MQ=255)                                                                                aGTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATTATAGCGCTCAACACGTTTGAAATTATAGGTCATCTCTGGTagg           <  1:3251804/100‑3 (MQ=255)                                                                                aGTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATTATAGCGCTCAACACGTTTGAAATTATAGGTCATCTCTGGTAtg           <  1:3433192/100‑2 (MQ=255)                                                                                aGTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATTATAGCGCTCAACACGTTTGAAATTATAGGTCATCTCTGGTAtg           <  1:1986489/100‑2 (MQ=255)                                                                                 gTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATTATAGCGCTCAACACGTTTGAAATTATAGGTCATCTCTGGtgctg          <  1:660198/100‑5 (MQ=255)                                                                                 gTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATTATAGCGCTCAACACGTTTGAAATTATAGGTCATCTCTGGTacaa          <  1:320089/100‑4 (MQ=255)                                                                                        aGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATTATAGCGCTCAACACGTTTGAAATTATAGGTCATCTCTGGTATTCTTGTACg   <  1:3851890/100‑1 (MQ=255)                                                                                         ggCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATTATAGCGCTCAACACGTTTGAAATTATAGGTCATCTCTGGTATTCTTGTAcat  <  1:3976095/100‑3 (MQ=255)                                                                                            |                                                                                                 CACGATTCGTAAAATCTGTTATCGAAATAATTCCTATATTATTGAAAATGAGCAACATCATCAATGCTAAGCCTAAAATAGTACCTGAGGCCAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATTATAGCGCTCAACACGTTTGAAATTATAGGTCATCTCTGGTATTCTTGTACGA  >  NC_007793/2015075‑2015262

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑